Effect of Staphylococcus aureus Thermostable DNase on Some Immunological Parameters

Abstract

Thermostable DNase was purified from Staphylococcus aureus isolated from local clinical source. The current study includes different immunological assays to evaluate the role of purified DNase. The phagocytic activity showed that the group of mice infected with P.aeruginosa recorded the highest values with significant differences (P <0.05) in comparison with the group that received mixed injection of 1 µg /ml of DNase and P. aeruginosa showed decrease in phagocytic activity followed by group that injected by 1 µg /ml DNase only with phagocytic activity 2%. DNase has a positive immunomodulatory effect on IL-10 and IL-4 level. The data showed that the highest value of IL-10 is in group that injected with 1 µg /ml DNase only (IV group) was 86.79±1.68. While the highest value of IL-4 was in group that injected with both DNase (1 µg /mouse) and P.aeruginosa gave the highest values (III group) was 84.02 ±1.19. In Arthus reactions(Immediate type hypersensitivity), the group that have been injected with both DNase and P. aeruginosa (group III ) gave the highest values (4.50±0.70) while in delayed type hypersensitivity the same group III showed higher values after 24 and 48 hrs (5.00±0.00) , (4.00±0.00) respectively.