Effect of steeping temperature on antioxidant and inhibitory activities of green tea extracts against α-amylase, α-glucosidase and intestinal glucose uptake

Liu, Shuyuan; Ai, Zeyi; Qu, Fengfeng; Chen, Yuqiong; Zhi, Yu

doi:10.1016/j.foodchem.2017.04.151

Cited by 69 publications

(37 citation statements)

References 36 publications

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“…Moreover, in comparison with the positive control, all PPPs exhibited strong α-glucosidase inhibitive effects and moderate α-amylase inhibitive effects. However, compared with polysaccharides obtained from okra [51], Momordica charantia [37], and green tea [52], both PPP-W and PPP-P exhibited higher α-amylase inhibition activities. Furthermore, the α-amylase and α-glucosidase inhibitory activities of PPPs were higher than those of polysaccharides obtained from Hovenia dulcis [10], but lower than those of flavonoids obtained from Hovenia dulcis [11], and the α-glucosidase and α-amylase inhibitive effects were also positively correlated with the contents of polysaccharides and flavonoids.…”

Section: In Vitro Inhibitory Activities On α-Amylase and α-Glucosidasementioning

confidence: 78%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

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Section: In Vitro Inhibitory Activities On α-Amylase and α-Glucosidasementioning

confidence: 78%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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“…Günümüzde kullanılan hipoglisemik ilaçlar, serum glikoz seviyesini normale döndürmeyi başarmakta ancak gastrointestinal rahatsızlıklar neden olmaktadır. Bu nedenle, α-Amilaz ve α-Glukozidaz'ı inhibe eden ve yan etkisi olmayan etkili terapötik ajanların bulunması önemlidir [42].…”

Section: Bulgular Ve Tartışmaunclassified

Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

Yırtıcı¹

2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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Bu çalışmada Centaurea fenzlii Reichardt bitkisinin antioksidan özellikleri ve çeşitli enzimler üzerine inhibe edici etkisi araştırıldı. Bu amaçla çiçeklenme döneminde toplanan Centaurea fenzlii Reichardt'ın toprak üstü kısımları farklı polariteye sahip çözücülerde maserasyon yöntemi kullanılarak çözüldü ve ekstreleri elde edildi. Çalışmalara metanol ekstresi ile devam edildi. Metanol ekstresinin toplam fenolik ve flavonoid içerikleri sırasıyla, 16,72 mg GAE/g ka ve 173,16 mg KAE/g ka olarak belirlendi. Antioksidan kapasiteleri demir indirgeyici gücü (FRAP) için 0,256 mmol TE/g ka, bakır indirgeyici gücü (CUPRAC) için 0,878 mmol TE/g ka, ABTS için 0,354 mmol TE/g ka ve DPPH için 0,661 mmol TE/g ka olarak saptandı. Ayrıca, ekstrelerinin kolinesteraz, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı inhibe edici etkileri de belirlendi. Enzim inhibisyon etkisi sırasıyla, α-Glukozidaz için 0,331 mmol AKE/g ka, α-Amilaz için 0,354 mmol AKE/g ka, AChE için 0,367 mmol GAE/g ka, BChE için 0,878 mmol GAE/g ka ve Tirozinaz için mmol 0,256 KE/g ka olarak bulundu.

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“…Such properties have been reported for polyphenols from coffee, guava, tea, whortleberry, olive oil, propolis, chocolate, red wine, grape seed, and cocoa [ 14 ]. Remarkably, polyphenols can inhibit digestive enzymes like α -amylase and α -glucosidase, leading to a reduced release of glucose after a meal [ 15 , 16 ]. In addition, polyphenols can modulate glucose transportation by stimulating GLUT transporters, among which the insulin-sensitive GLUT4 is notable.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract

Shakoor

Abdelfattah

Albadi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Type 2 diabetes mellitus is increasing worldwide, and the United Arab Emirates is presenting one of the world’s highest prevalence rates. Dietary polyphenols exert an antidiabetic effect by modulating carbohydrates digestion and cellular glucose uptake. Due to their particularly high content in polyphenols, date seeds represent a potential antidiabetic agent. This study aims to determine if date seed polyphenols inhibit the activity of the enzymes (α-amylase and α-glucosidase), responsible for the digestion of carbohydrates and modulating the glucose uptake by human liver cells. In vitro activity of the intestinal α-glucosidase, pancreatic α-amylase, the glucose uptake by HepG2 cells, and the expression of GLUT4 and AMPK analyzed by western blotting (with and without date seeds extract). Our result showed that the maximum enzymes inhibition was obtained with 400 μg/mL and 900 μg/mL DSE for α-amylase and α-glucosidase, respectively. The HepG2 cell viability significantly decreased up to 80% at 4000 μg/mL DSE. The expression of GLUT4 was higher at 100 μg/mL DSE (with insulin and without insulin). However, the expressions of P-AMPK and AMPK were increased by DSE, mainly in a non-insulin-dependent manner. Therefore, DSE, by inhibiting carbohydrate digestion and stimulating glucose uptake by HepG2, can potentially demonstrate the therapeutic potential for diabetes management.

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Effect of steeping temperature on antioxidant and inhibitory activities of green tea extracts against α-amylase, α-glucosidase and intestinal glucose uptake

Cited by 69 publications

References 36 publications

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract

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