Effect of sterilization on the properties of CDHA-OCP-beta-TCP biomaterial

Abstract

The effect of the method of sterilization on the physical, chemical and mechanical properties of a new bone repairing material was studied. The material was obtained by thermal hydrolysis of beta-tricalcium phosphate/orthophosphoric acid cement and was composed of calcium deficient hydroxyapatite, octacalcium phosphate (OCP), and beta-tricalcium phosphate. Partial decomposition of the OCP was observed after sterilization for the three methods. Decomposition increased to the following sequence of sterilization methods: ethylene oxide; autoclaving; dry oven. On the other hand, mechanical strength decreased with regard to non sterilized material in the sterilization sequence: ethylene oxide; dry oven; autoclaving. The compressive strength was 8.5 ± 1.0; 9.0 ± 1.2; 8.2 ± 0.8 and 6.5 ± 1.0 MPa, whereas diametral tensile strength was 2.1 ± 0.3; 2.5 ± 0.1; 1.9 ± 0.9 and 1.6 ± 0.3 for the material sterilized by ethylene oxide, dry oven, and autoclaving, respectively. Several compositional and microstuctural changes were detected after dry heat and autoclave sterilization. Ethylene oxide sterilization had lesser effect on the chemical composition and strength than dry heat and autoclaving

Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration

“…HA nanocrystals were precipitated on the BC nanofibrils as agglomerates of crystallites Figure 4(a) ; regularly distributed pores were observed at the membrane surface. Typical HA crystals in globular and rod form were observed, similar to the HA crystals as has also been observed by other authors [ 31 , 32 ]. These crystals grew laterally around the nanofibres.…”

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

“…g-irradiation has been widely used in the sterilization of numerous biomaterials and milk [146][147][148][149][150]. Appropriate doses of irradiation are effective against several bacteria, viruses and fungi; however, it has been found to affect the biological properties and tissue integrity [151,152].…”

Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell‐based bone tissue engineering

“…Alginate scaffolds (diameter ¼ 10.19 6 0.13 mm, thickness ¼ 3.0 6 0.5 mm) were mineralized for 3 weeks, rinsed to eliminate residual salts, dried and sterilized using ethylene oxide gas which has been shown to have a less significant effect on the chemical composition and strength of other calcium-and phosphorous-based minerals than dry heat and autoclaving. 25 Before cell seeding, the scaffolds were soaked in DMEM supplemented with 0.3 g/L CaCO 3 overnight. Human mesenchymal stem cells (hMSCs) purchased from Cambrex (Baltimore, MD) were expanded on tissue culture polystyrene plates according to the protocol provided by the supplier.…”