Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor

Lopez-Ramirez, N.; Volke-Sepúlveda, Tania; Gaime-Perraud, Isabelle; Saucedo‐Castañeda, Gerardo; Favela‐Torres, Ernesto

doi:10.1016/j.biortech.2018.06.015

Cited by 29 publications

(15 citation statements)

References 35 publications

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“…harzianum produced the highest activity (149.3 ± 14.3 IU/ gdm in 108 h), and this result is very similar to that described by López-Ramírez et al (2018) who reported up to 100 IU/ gdm produced by T. harziamum grown on pine sawdust but in conditions of aeration and agitation, Rezende et al (2002) detected 36 IU/mL of xilanolytic activity of T. harzianum using bagasse from sugarcane as a substrate; on the other hand, Rahnama et al (2013) reported 433 IU/gms using rice straw as a substrate. It is clear that the substrate is essential to induce xilanolytic activity since both rice straw and WH have a high content of hemicelluloses, whereas for β-dxylopyranosidase activity (Fig.…”

Section: Saccharifying Enzyme Production By the Filamentous Fungi Grosupporting

confidence: 88%

Use of water hyacinth as a substrate for the production of filamentous fungal hydrolytic enzymes in solid-state fermentation

et al. 2019

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The objective of the present work was to evaluate the water hyacinth (WH) as a substrate for the production of hydrolytic enzymes (cellulases and hemicellulases) of 100 strains of filamentous fungi under conditions of solid growth. Five fungal strains, identified as Trichoderma harzianum, Trichoderma atroviride, Penicillium griseofulvum, Penicillium commune and Aspergillus versicolor, were selected and studied for their ability to grow on water hyacinth as a substrate and carbon source only, evaluating hydrolytic enzymatic activities (α-l-arabinofuranosidase, cellulase, xylanase and β-d-xylopyranosidase) and extracellular protein per g of water hyacinth dry matter (gdm). The five strains selected were able to produce the four enzymes studied; however, T. harzianum strain PBCA produces the highest xylanase (149.3 ± 14.3 IU/gdm at 108 h), cellulase (16.4 ± 0.6 IU/gdm at 84 h) and β-d-xylopyranosidase (127.7 ± 14.8 IU/gdm at 48 h). In contrast, the fungus with the highest α-l-arabinofuranosidase activity was A. versicolor, with 129.8 ± 13.3 IU/gdm after 108 h. In conclusion, T. harzianum showed the best production of the hydrolytic enzymes studied, using as a matrix and carbon source, water hyacinth. In addition, catalytic activities of arabinofuranosidase and xylopyranosidase were reported for the first time in T. versicolor and T. harzianum.

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Section: Saccharifying Enzyme Production By the Filamentous Fungi Grosupporting

confidence: 88%

Use of water hyacinth as a substrate for the production of filamentous fungal hydrolytic enzymes in solid-state fermentation

et al. 2019

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“…The lower productions of degradation product may be due, in this process, to a reduced production of hydrolases at higher scale. Lopez-Ramirez et al [32], observed that the application of an agitation drastically diminished the cellulases production in comparison to their static conditions. The fact that productions of fonsecin and ustilaginoidin A started after two days of growth in lab scale and not in the PFB is the consequence of the RTD due to mixing and the adding of fermented materiel as inoculum in the first compartment leading to presence of these metabolites in the first compartments of the bioreactor.…”

Section: Scales Comparisonmentioning

confidence: 99%

Solid state fermentation pilot-scaled plug flow bioreactor, using partial least square regression to predict the residence time in a semicontinuous process

Carboué

Rébufa

Dupuy

et al. 2019

Biochemical Engineering Journal

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Semicontinuous production of fungal compounds was obtained with a Plug Flow Bioreactor (PFB) prototype. • Productions of same metabolites obtained over time at lab scale were used to build a Partial Least Square (PLS) model. • A residence time of solid particles was associated to each PFB compartment using the PLS model. • The PFB prototype was suitable to continuously produce fungal secondary metabolites.• PLS was an innovative approach to evaluate the scale-up of the metabolite production. A R T I C L E I N F O Keywords: Plug flow bioreactor Solid state fermentation Partial least square regression Residence time Aspergillus niger A B S T R A C T Plug flow bioreactor (PFB) used in solid state fermentation gives the possibility to have semicontinuous culture.However, it is complicated to follow a single particle during its residence inside the bioreactor and to therefore study precisely the culture process all along the device. In this study, semicontinuous production of fungal compounds was successfully obtained by cultivating Aspergillus niger with a PFB prototype. Kinetic productions of the same metabolites along the bioreactor were obtained at laboratory scale to predict the residence time of the particles inside the PFB from Partial Least Square (PLS). This original methodology allowed (1) the comparison between the production kinetics at the two production scales (pilot and laboratory) and (2) the observation of eventual changes following the scale-up of the process offering a good overall insight of the PFB performance.

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“…xylanase, β-xylosidase α-glucuronidase, α-arabinofuranosidase, and acetyl xylan esterase (Lopez-Ramirez et al, 2018). The synergistic cooperation between cellulolytic and hemicellulolytic enzymes helps in the sacchari cation of lignocellulosic materials (Brijwani et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Solid-state cultivation of Aspergillus niger–Trichoderma reesei from sugarcane bagasse with vinasse in bench packed-bed column bioreactor

Rocha

Campanhol

Bastos

2021

Preprint

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Solid-state cultivation (SSC) is microbial growth on solid supports under limited water conditions. Citric acid, one of the products obtained by SSC, is a microbial aerobic metabolic product with various industrial applications. Several wastes from agro-industries are used in SSC, such as sugarcane bagasse and vinasse. As xylanolytic enzymes of inoculum breakdown the lignocellulosic material (bagasse), mixed fungal cultures or co-cultures are used in these SSC. Thus, this study aims to evaluate the effect of inoculum (Aspergillus niger and Trichoderma reesei consortium) in the production of citric acid from sugarcane bagasse impregnated with vinasse using bench packed bed reactors (PBR). The results show the importance of T. reesei in inoculum with A. niger at a ratio of 50:50 and 25:75, suggesting the use of solid support due to the complementation of the hydrolytic enzymes. The highest concentration of approximately 1000 mg L− 1 of citric acid yield for 100 mm of bed height in 48 and 72 h, with the maximum yield from glucose to citric acid (2.2 mg citric acid mg glucose−1). kLa indicates that maintaining solid moisture and liquid film thickness is important to keep the oxygen transfer in SSC.

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Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor

Cited by 29 publications

References 35 publications

Use of water hyacinth as a substrate for the production of filamentous fungal hydrolytic enzymes in solid-state fermentation

Use of water hyacinth as a substrate for the production of filamentous fungal hydrolytic enzymes in solid-state fermentation

Solid state fermentation pilot-scaled plug flow bioreactor, using partial least square regression to predict the residence time in a semicontinuous process

Solid-state cultivation of Aspergillus niger–Trichoderma reesei from sugarcane bagasse with vinasse in bench packed-bed column bioreactor

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