Effect of storage conditions on maintaining anammox cell viability during starvation and recovery

Ganesan, Sivarajah; Vadivelu, Vel Murugan

doi:10.1016/j.biortech.2019.122341

Cited by 34 publications

(3 citation statements)

References 34 publications

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“…The entire reaction only needs 120 h. These experimental results preliminarily demonstrated that N 2 H 4 can reduce the mortality of anammox bacteria and enhance the anammox recovery process. 13,26 N 2 H 4 was easily oxidized by anammox bacteria, which provided additional metabolized energy, helping to cut down the start-up time of the anammox process. 15 The key to maintain cell viability depends on the anammox metabolic reaction pathway for degrading substrates.…”

Section: Resultsmentioning

confidence: 99%

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Chen¹,

zhou²,

Wang³

et al. 2022

Environ. Sci.: Water Res. Technol.

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Section: Resultsmentioning

confidence: 99%

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Chen¹,

zhou²,

Wang³

et al. 2022

Environ. Sci.: Water Res. Technol.

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“…The presence of complete pathways for denitrification and nitrification indicates potential for these habitats to sustain levels of nitrite and ammonium that would enable the anammox reaction [ 22 ]. Additionally, wastewater treatment facilities represent systems that contain high concentrations of nitrate, nitrite, and ammonium, yet anammox bacteria are not typically naturally present [ 44 , 45 ]. Instead, they are added to these systems, typically within a bioreactor that provides sufficiently low reduction potential to support the growth of these bacteria [ 46 – 49 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiomes and Planctomycete diversity in large-scale aquaria habitats

et al. 2022

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In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria.

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“…Previous research on optimally storing anaerobic granular sludge experiments has focused on conditions such as low temperatures, short periods, and the addition of substrates or protectants. Few studies have been reported on the feasibility of the recovery of the bacterial population and comparative studies of different sludge start-ups after intermittent shutdowns, equipment maintenance, natural disasters, and other unexpected conditions that bring irresistible external resistance leading to prolonged reactor outages, causing long-term starvation of AAOB and affecting their activity [8,9] in engineering practice.…”

Section: Introductionmentioning

confidence: 99%

Rapid Start-Up Characteristics of Anammox under Different Inoculation Conditions

Tan

Xia

et al. 2023

IJERPH

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The long multiplication time and extremely demanding enrichment environment requirements of Anammox bacteria (AAOB) have led to difficult reactor start-ups and hindered its practical dissemination. Few feasibility studies have been reported on the recovery of AAOB activity initiation after inlet substrate disconnection caused by an unfavorable condition, and few factors, such as indicators of the recovery process, have been explored. Therefore, in this experiment, two modified expanded granular sludge bed reactors (EGSB) were inoculated with 1.5 L anaerobic granular sludge (AGS) + 1 L Anammox sludge (AMS) (R1) and 2.5 L anaerobic granular sludge (AGS) (R2), respectively. After a long-term (140 days) starvation shock at a high temperature (38 °C), the bacteria population activity recovery experiments were conducted. After 160 days, both reactors were successfully started up, and the total nitrogen removal rates exceeded 87%. Due to the experimental period, the total nitrogen removal rate of R2 was slightly higher than that of R1 in the final stage. However, it is undeniable that R2 had a relatively long activity delay during startup, while R1 had no significant activity delay during startup. The sludge obtained from R1 had a higher specific anammox activity (SAA). Analysis of the extracellular polymer substances (EPS) results showed that the extracellular polymer content in R1 was higher than that in R2 throughout the recovery process, indicating that R1 had higher sludge stability and denitrification performance. Scanning electron microscopy (SEM) analysis showed that more extracellular filamentous bacteria could be seen in the R1 reactor with better morphology of Anammox bacteria. In contrast, the R2 reactor had fewer extracellular hyphae and micropores as a percentage and higher filamentous bacteria content. The results of microbial 16SrDNA analysis showed that R1 used AAOB as inoculum to initiate Anammox, and the reactor was enriched with Anammox bacteria earlier and in much greater abundance than R2. The experimental results indicated that inoculating mixed anaerobic granular sludge and Anammox sludge to initiate an anammox reactor was more effective.

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Effect of storage conditions on maintaining anammox cell viability during starvation and recovery

Cited by 34 publications

References 34 publications

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Recovery of anaerobic ammonium oxidation via hydrazine following sulfate inhibition

Microbiomes and Planctomycete diversity in large-scale aquaria habitats

Rapid Start-Up Characteristics of Anammox under Different Inoculation Conditions

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