Effect of Streptozotocin on Plasma Insulin Levels of Rats and Mice: A Meta-analysis Study

Köksal, Burcu

doi:10.3889/oamjms.2015.093

Cited by 25 publications

(14 citation statements)

References 20 publications

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“…). However, this concentration was supraphysiological (Koksal, ) and it is unknown whether the results will be applicable to lower, more physiological doses of insulin. Second, we did not consider any effects of the change in extracellular osmotic pressure induced by the insulin application on cell membrane tension in patch clamp experiments.…”

Section: Discussionmentioning

confidence: 99%

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Hotta

Katanosaka

Mizumura

et al. 2019

The Journal of Physiology

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Key points Insulin is known to activate the sympathetic nervous system centrally. A mechanical stimulus to tissues activates the sympathetic nervous system via thin fibre afferents. Evidence suggests that insulin modulates putative mechanosensitive channels in the dorsal root ganglion neurons of these afferents. In the present study, we report the novel finding that insulin augments the mechanical responsiveness of thin fibre afferents not only at dorsal root ganglion, but also at muscle tissue levels. Our data suggest that sympathoexcitation is mediated via the insulin‐induced mechanical sensitization peripherally. The present study proposes a novel physiological role of insulin in the regulation of mechanical sensitivity in somatosensory thin fibre afferents. Abstract Insulin activates the sympathetic nervous system, although the mechanism underlying insulin‐induced sympathoexcitation remains to be determined. A mechanical stimulus to tissues such as skin and/or skeletal muscle, no matter whether the stimulation is noxious or not, activates the sympathetic nervous system via thin fibre afferents. Evidence suggests that insulin modulates putative mechanosensitive channels in the dorsal root ganglion (DRG) neurons of these afferents. Accordingly, we investigated whether insulin augments whole‐cell current responses to mechanical stimuli in small DRG neurons of normal healthy mice. We performed whole‐cell patch clamp recordings using cultured DRG neurons and observed mechanically‐activated (MA) currents induced by mechanical stimuli applied to the cell surface. Local application of vehicle solution did not change MA currents or mechanical threshold in cultured DRG neurons. Insulin (500 mU mL−1) significantly augmented the amplitude of MA currents (P < 0.05) and decreased the mechanical threshold (P < 0.05). Importantly, pretreatment with the insulin receptor antagonist, GSK1838705, significantly suppressed the insulin‐induced potentiation of the mechanical response. We further examined the impact of insulin on thin fibre muscle afferent activity in response to mechanical stimuli in normal healthy rats in vitro. Using a muscle–nerve preparation, we recorded single group IV fibre activity to a ramp‐shaped mechanical stimulation. Insulin significantly decreased mechanical threshold (P < 0.05), although it did not significantly increase the response magnitude to the mechanical stimulus. In conclusion, these data suggest that insulin augments the mechanical responsiveness of small DRG neurons and potentially sensitizes group IV afferents to mechanical stimuli at the muscle tissue level, possibly contributing to insulin‐induced sympathoexcitation.

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Section: Discussionmentioning

confidence: 99%

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Hotta

Katanosaka

Mizumura

et al. 2019

The Journal of Physiology

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“…Figure 8 speaks to the adequacy of chloroform and methanol concentrates of Barleria cuspidata on serum insulin and glycosylated hemoglobin levels. Streptozotocin causes an irregular β-cell working by weakening glucose oxidation and diminishing glucose biosynthesis and discharge (Koksal, 2015). In the current examination on the organization of streptozotocin diminishes the serum insulin levels in diabetic rats, contrasted and normal rats speak to the β-cell damage.…”

Section: Figure 1: Effectof Cebc and Mebc Treatment On Blood Glucose Level In Normoglycemic Ratsmentioning

confidence: 84%

Antidiabetic and antihyperlipidemic activities of extracts of Barleria cuspidata Heyne ex Nees on streptozotocin induced diabetic rats

Reddy¹,

Sundararajan

2021

ijrps

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Traditionally, Barleria cuspidata Heyne ex Nees is utilized for antidiabetic action with the absence of logical investigation. Thus, the current examination was attempted to explore for its antidiabetic and antihyperlipidemic movement in streptozotocin instigated diabetic animal models. Blood glucose levels were estimated in normoglycemic rats at initial, 60th and 120th minutes intervals and in glucose feed hyperglycemic rats at initial, 30, 60, 90 and 120 minutes after a solitary portion of streptozotocin at 55 mg/kg body weight intraperitoneal were made diabetic in albino rats. Blood glucose levels were estimated at week by week spans after everyday administration of chloroform and methanol extracts of Barleria cuspidata at dosages of 250 and 500 mg/kg body weight. Other biochemical boundaries of serum triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, total protein, albumin, globulin, uric acid, creatinine, urea, transaminases, alkaline phosphatase, alanine aminotransaminase, insulin and glycosylated hemoglobin were likewise estimated toward the finish of the investigation. Chloroform and methanol extracts of Barleria cuspidata by an oral organization for 21 days altogether (P<0.001) decreases the elevated blood glucose extents in diabetic rats whereas in normoglycemic rats it doesn't adjust the blood glucose amounts altogether and in glucose feed hyperglycemic rats significantly decreases the raised blood glucose levels. Likewise, the chloroform and methanol extracts of Barleria cuspidata improved other biochemical boundaries related to diabetes. Moreover, the extracts of Barleria cuspidata favourable affect the histopathological changes of pancreas in streptozotocin initiated diabetic rats. Delayed consequences legitimize the traditional utilization of Barleria cuspidata for its anti-diabetic action.

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“…STZ-induced beta pancreatic Langerhans cell damage thus impaired insulin stimulation for muscle metabolism [13]. Plasma insulin level reduced significantly after 5 consecutive days in mouse [14].…”

Section: Discussionmentioning

confidence: 99%

Golden sea cucumber extract revives glucose transporter-4 and interleukin-6 protein level in diabetic mouse muscle

et al. 2019

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Background: Streptozotocin (STZ)-induced free radical oxidant activity resulted in muscle wasting due to protein carbonyl (PC), glucose transporter-4 (Glut-4), and interleukin-6 (IL-6) protein alteration. Antioxidant ingredient in the golden sea cucumber extract was found in promising level to inhibit free radical activity. Aim: This study was aimed to investigate the effect of golden sea cucumber extract on PC, IL-6, and Glut-4 level of STZ-induced diabetes mouse. Materials and Methods: This study was performed using mice, which were grouped into non-diabetes, diabetes, and diabetes-treated extract groups. The golden sea cucumber was extracted using 70% ethanol, which was administered by oral gavage twice a day for 5 consecutive days. Results: The extract reduced PC level and improved muscle Glut-4 and IL-6 protein level of diabetic mouse. Conclusion: The extract of golden sea cucumber revived muscle Glut-4 and IL-6 protein level in protection against muscle wasting.

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Effect of Streptozotocin on Plasma Insulin Levels of Rats and Mice: A Meta-analysis Study

Cited by 25 publications

References 20 publications

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Antidiabetic and antihyperlipidemic activities of extracts of Barleria cuspidata Heyne ex Nees on streptozotocin induced diabetic rats

Golden sea cucumber extract revives glucose transporter-4 and interleukin-6 protein level in diabetic mouse muscle

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