Effect of strontium substituted ß‐TCP associated to mesenchymal stem cells from bone marrow and adipose tissue on spinal fusion in healthy and ovariectomized rat

Salamanna, Francesca; Giavaresi, G.; Contartese, Deyanira; Bigi, Adriana; Boanini, Elisa; Parrilli, Annapaola; Lolli, Roberta; Gasbarrini, A.; Bròdano, Giovanni Barbanti; Fini, Milena

doi:10.1002/jcp.28601

Cited by 29 publications

(22 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, their beneficial effect on bone cells has also been observed when coupled to β-TCP [20,21,22,23,24]. In particular, strontium-substituted β-TCP (SrTCP) added with mesenchymal stem cells has been shown to improve bone formation and fusion across the transverse processes, both in osteoporotic and non-osteoporotic animal models [25]. The possible substitution of these two cations into β-TCP structure has been previously investigated.…”

Section: Introductionmentioning

confidence: 99%

Strontium and Zinc Substitution in β-Tricalcium Phosphate: An X-ray Diffraction, Solid State NMR and ATR-FTIR Study

Boanini

Gazzano

Nervi

et al. 2019

JFB

Self Cite

View full text Add to dashboard Cite

β-tricalcium phosphate (β-TCP) is one of the most common bioceramics, widely applied in bone cements and implants. Herein we synthesized β-TCP by solid state reaction in the presence of increasing amounts of two biologically active ions, namely strontium and zinc, in order to clarify the structural modifications induced by ionic substitution. The results of X-ray diffraction analysis indicate that zinc can substitute for calcium into a β-TCP structure up to about 10 at% inducing a reduction of the cell parameters, whereas the substitution occurs up to about 80 at% in the case of strontium, which provokes a linear increase of the lattice constants, and a slight modification into a more symmetric structure. Rietveld refinements and solid-state 31P NMR spectra demonstrate that the octahedral Ca(5) is the site of β-TCP preferred by the small zinc ion. ATR-FTIR results indicate that zinc substitution provokes a disorder of β-TCP structure. At variance with the behavior of zinc, strontium completely avoids Ca(5) site even at high concentration, whereas it exhibits a clear preference for Ca(4) site. The infrared absorption bands of β-TCP show a general shift towards lower wavenumbers on increasing strontium content. Particularly significant is the shift of the infrared symmetric stretching band at 943 cm−1 due to P(1), that is the phosphate more involved in Ca(4) coordination, which further supports the occupancy preference of strontium.

show abstract

Section: Introductionmentioning

confidence: 99%

Strontium and Zinc Substitution in β-Tricalcium Phosphate: An X-ray Diffraction, Solid State NMR and ATR-FTIR Study

Boanini

Gazzano

Nervi

et al. 2019

JFB

Self Cite

View full text Add to dashboard Cite

show abstract

“…Results reported from preclinical studies are confirming that BMA clot could be able to perform the necessary physiological functions to achieve and facilitate cartilage and bone tissue regeneration in patients [218]. Recently, we also demonstrated the better efficiency of BM-MSC with respect to Ad-MSC in a preclinical study performed on a rat model of spinal fusion, using a strontium substituted β-tricalcium phosphate as a scaffold, associated with unexpanded and undifferentiated MSC [227].…”

Section: Challenges In Msc Translation Into Clinical Practice: Thementioning

confidence: 64%

Substantial Overview on Mesenchymal Stem Cell Biological and Physical Properties as an Opportunity in Translational Medicine

Abdelrazik

Giordano

Bròdano

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Mesenchymal stem cells (MSC) have piqued worldwide interest for their extensive potential to treat a large array of clinical indications, their unique and controversial immunogenic and immune modulatory properties allowing ample discussions and debates for their possible applications. Emerging data demonstrating that the interaction of biomaterials and physical cues with MSC can guide their differentiation into specific cell lineages also provide new interesting insights for further MSC manipulation in different clinical applications. Moreover, recent discoveries of some regulatory molecules and signaling pathways in MSC niche that may regulate cell fate to distinct lineage herald breakthroughs in regenerative medicine. Although the advancement and success in the MSC field had led to an enormous increase in the amount of ongoing clinical trials, we still lack defined clinical therapeutic protocols. This review will explore the exciting opportunities offered by human and animal MSC, describing relevant biological properties of these cells in the light of the novel emerging evidence mentioned above while addressing the limitations and challenges MSC are still facing.

show abstract

“…Several studies to date have shown that ASCs can induce spinal fusion in various preclinical models; however, the majority of these studies either use genetically modified cells, [20][21][22]24 cells of a higher passage number (≥P4), 20,21,23,25 immunocompromised recipients, [20][21][22][24][25][26] experimental carriers, [27][28][29][30] or some combination therein, thus limiting their clinical relevance. Our study circumvents many of these issues by utilizing low passage number rASCs and rBMCs from the same donor animals, a clinical-grade bone graft substitute, and immune intact syngeneic recipient animals.…”

Section: Discussionmentioning

confidence: 99%

“…More importantly, studies have suggested that ASCs are less influenced by aging and skeletal conditions than marrow-derived progenitors. [17][18][19] However, the use of ASCs in spinal fusion procedures is only beginning to be explored, [20][21][22][23][24][25][26][27][28][29][30][31][32] with limited research directly comparing their performance to BMCs. 21,30 In this study, we thus directly compare the efficacy of ASCs and BMCs in achieving successful spinal fusion when combined with a clinical-grade bone graft substitute in a rat model.…”

mentioning

confidence: 99%

Comparing the efficacy of adipose‐derived and bone marrow‐derived cells in a rat model of posterolateral lumbar fusion

Holmes

Ishida

Perdomo‐Pantoja

et al. 2021

Journal Orthopaedic Research

View full text Add to dashboard Cite

Although bone marrow-derived mesenchymal stem cells (BMCs) have been widely used in spinal fusion procedures, adipose-derived stem cells (ASCs) offer a number of advantages as an alternative clinical cell source. This study directly compares the efficacy of ASCs and BMCs from the same donor animals to achieve successful fusion when combined with a clinical-grade bone graft substitute in a rat lumbar fusion model. ASCs and BMCs were isolated from the same Lewis donor rats and grown to passage 2 (P2).Single-level bilateral posterolateral intertransverse process lumbar fusion surgery was performed on syngeneic rats divided into three experimental groups: clinical-grade bone graft substitute alone (CBGS); CBGS+ rat ASCs (rASC); and, CBGS+ rat BMCs (rBMC).Eight weeks postoperatively, fusion was evaluated via micro-CT, manual palpation and histology. In vitro analysis of the osteogenic capacity of rBMCs and rASCs was also performed. Results indicated that the average fusion volume in the rASC group was the largest and was significantly larger than the CBGS group. Although the rASC group displayed the highest fusion rates via micro-CT and manual palpation, this difference was not statistically significant. Cell-seeded grafts showed more histological bone formation than cell-free grafts. P2 rASCs and rBMCs displayed similar in vitro osteogenic differentiation capacities. Overall, this study showed that, when combined with a clinical-grade bone graft substitute in a rat model, rASCs cells yielded the largest fusion masses and comparable fusion results to rBMCs. These results add to growing evidence that ASCs provide an attractive alternative to BMCs for spinal fusion procedures.adipose stem cell, animal model, bone marrow cell, mesenchymal stem cell, spine fusion | INTRODUCTIONSpinal fusion is an increasingly common procedure used to treat a variety of conditions arising from trauma, degenerative diseases, deformity, infection, and tumors. While generally successful, the current "gold standard" of care, which involves the use of autograft bone, still fails in up to 35% of cases, resulting in significant patient morbidity, the need for additional procedures, and increased health care costs. 1 A wide variety of clinical bone graft substitutes, extension systems and orthobiologics have thus been developed to

show abstract

Effect of strontium substituted ß‐TCP associated to mesenchymal stem cells from bone marrow and adipose tissue on spinal fusion in healthy and ovariectomized rat

Cited by 29 publications

References 41 publications

Strontium and Zinc Substitution in β-Tricalcium Phosphate: An X-ray Diffraction, Solid State NMR and ATR-FTIR Study

Strontium and Zinc Substitution in β-Tricalcium Phosphate: An X-ray Diffraction, Solid State NMR and ATR-FTIR Study

Substantial Overview on Mesenchymal Stem Cell Biological and Physical Properties as an Opportunity in Translational Medicine

Comparing the efficacy of adipose‐derived and bone marrow‐derived cells in a rat model of posterolateral lumbar fusion

Contact Info

Product

Resources

About