Effect of strontium substitution on functional activity of phosphate-based glass

Ryu, Jeong-Hyun; Mangal, Utkarsh; Lee, Myung-Jin; Seo, Ji‐Young; Jeong, Il Jun; Park, Jin‐Young; Na, Ji-Yeong; Lee, Kee‐Joon; Yu, Hyung Seog; Cha, Jae‐Kook; Kwon, Jae‐Sung; Choi, Sung‐Hwan

doi:10.1039/d3bm00610g

Cited by 6 publications

(1 citation statement)

References 45 publications

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“…Strontium-doped phosphate glass has the potential for active ion delivery and promotion of bone formation [ 11 , 12 ]. Previous research succeeded in making phosphate glasses with up to 5 mol% of strontium which showed low cytotoxicity [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Titanium-doped phosphate glasses containing zinc and strontium applied in bone regeneration

Tang,

Wandless,

Keskin-Erdogan

et al. 2024

J Mater Sci: Mater Med

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Phosphate bioactive glass has been studied for its advanced biodegradability and active ion release capability. Our previous research found that phosphate glass containing (P2O5)–(Na2O)–(TiO2)–(CaO)–(SrO) or (ZnO) showed good biocompatibility with MG63 and hMSCs. This study further investigated the application of 5 mol% zinc oxide or 17.5 mol% strontium oxide in titanium-doped phosphate glass for bone tissue engineering. Ti-Ca-Na-Phosphate glasses, incorporating 5% zinc oxide or 17.5% strontium oxide, were made with melting quenching technology. The pre-osteoblast cell line MC3T3-E1 was cultured for indirect contact tests with graded diluted phosphate glass extractions and for direct contact tests by seeding cells on glass disks. The cell viability and cytotoxicity were analysed in vitro over 7 days. In vivo studies utilized the tibial defect model with or without glass implants. The micro-CT analysis was performed after surgery and then at 2, 6, and 12 weeks. Extractions from both zinc and strontium phosphate glasses showed no negative impact on MC3T3-E1 cell viability. Notably, non-diluted Zn-Ti-Ca-Na-phosphate glass extracts significantly increased cell viability by 116.8% (P < 0.01). Furthermore, MC3T3-E1 cells cultured with phosphate glass disks exhibited no increase in LDH release compared with the control group. Micro-CT images revealed that, over 12 weeks, both zinc-doped and strontium-doped phosphate glasses demonstrated good bone incorporation and longevity compared to the no-implant control. Titanium-doped phosphate glasses containing 5 mol% zinc oxide, or 17.5 mol% strontium oxide have promising application potential for bone regeneration research. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Titanium-doped phosphate glasses containing zinc and strontium applied in bone regeneration

Tang,

Wandless,

Keskin-Erdogan

et al. 2024

J Mater Sci: Mater Med

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Integrating Phosphate Enhances Biomineralization Effect of Methacrylate Cement in Vital Pulp Treatment with Improved Human Dental Pulp Stem Cells Stimulation

Ryu,

Mangal,

Kwon

et al. 2024

Adv Healthcare Materials

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Vital pulp treatment (VPT) is crucial for preserving the health and function of the tooth in cases where the pulp tissue remains vital despite exposure. Various materials are introduced for this purpose. However, challenges such as low strength, high solubility, and tooth discoloration persist. Methylmethacrylate‐based cement (MC) offers excellent sealing ability, feasibility, and mechanical properties, making it a promising alternative for VPT. Phosphate‐based glass (PBG) has the potential to promote hard tissue regeneration by releasing key inducers, phosphorus (P) and calcium (Ca), for reparative odontogenesis. This study investigates PBG‐integrated MC (PIMC) by characterizing its properties, assessing human dental pulp stem cell activity related to initial inflammatory adaptation and odontogenic differentiation, and evaluating hard tissue formation using an in vivo dog pulpotomy model. Results indicate that a 5% PBG‐integrated MC (5PIMC) maintains the physicochemical properties of MC. Furthermore, 5PIMC demonstrates cytocompatibility, excellent expression of osteo/odontogenic markers, and resistance to inflammatory markers, significantly outperforming MC. Enhanced hard tissue formation is observed in the dental pulp of mongrel dog teeth treated with 5PIMC. These findings suggest that 5PIMC could be an optimal and suitable material for reparative odontogenesis through VPT.

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Zinc phosphate glass microspheres promoted mineralization and expression of BMP2 in MC3T3‐E1 cells

Tang,

Kang,

Verisqa

et al. 2024

J Biomedical Materials Res

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Degradable phosphate glasses have shown favorable properties for tissue engineering. By changing the composition of the glasses, the degradation rate, and ion release are controllable. Zinc oxide can function as a glass network modifier and has been shown to play a positive role in bone formation. Also, phosphate glasses can easily be processed into microspheres, which can be used as microcarriers. This study aims to develop zinc phosphate glasses microspheres and explore the optimized size and composition for applications in bone tissue engineering. Zinc–titanium–calcium–sodium phosphate glasses with 0, 1, 3, 5, or 10 mol % zinc oxide were prepared and processed into microspheres. The smaller microspheres ranged in size from 50 to 106 μm, while the larger ones ranged from 106 to 150 μm. The characteristics of glasses were examined. The osteoblastic cell line MC3T3‐E1 was cultured on the surface of microspheres and the cell viability was examined. To evaluate osteogenic differentiation, Alizarin Red S staining, quantitative reverse transcription polymerase chain reaction, and western blot analysis were performed after 14 days. Different sizes of zinc phosphate glass microspheres were successfully made. The glass microspheres with <10 mol % zinc oxide were able to support the adhesion and proliferation of MC3T3‐E1 cell lines. The relative gene expression of BMP2 was significantly upregulated in the smaller glass microspheres containing 3 mol % zinc oxide (26‐fold, p < .001) and both sizes of microspheres containing 5 mol % zinc oxide (smaller: 27‐fold, p < .001; larger: 35‐fold, p < .001). Additionally, cluster formation was observed in glass microspheres after 14 days, and the mineralization of MC3T3‐E1 cell lines was promoted. Based on these findings, the glass microspheres containing 3–5 mol % of zinc oxide can promote osteogenic differentiation for MC3T3‐E1 cells.

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Effect of strontium substitution on functional activity of phosphate-based glass

Abstract: The optimization of strontium-substituted phosphate-based glass (PSr) can augment the bioactive cellular response by enhancing osteogenic differentiation and resisting the pro-inflammatory cytokine and improve the regenerative healing response.

Cited by 6 publications

References 45 publications

Titanium-doped phosphate glasses containing zinc and strontium applied in bone regeneration

Titanium-doped phosphate glasses containing zinc and strontium applied in bone regeneration

Integrating Phosphate Enhances Biomineralization Effect of Methacrylate Cement in Vital Pulp Treatment with Improved Human Dental Pulp Stem Cells Stimulation

Zinc phosphate glass microspheres promoted mineralization and expression of BMP2 in MC3T3‐E1 cells

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