Effect of sub-inhibitory concentrations of biocides on the architecture and viability of MRSA biofilms

Buzón-Durán, Laura; Alonso‐Calleja, Carlos; Riesco-Peláez, Félix; Capita, Rosa

doi:10.1016/j.fm.2017.01.003

Cited by 50 publications

(37 citation statements)

References 33 publications

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“…However, a regulatory impact on c-di-GMP metabolism in P. aeruginosa has only been described for some agents including tobramycin and SDS and both toxic agents promote a sessile mode of growth of P. aeruginosa when applied at sublethal concentrations ( Hoffman et al, 2005 ; Klebensberger et al, 2009 ). So far, the only studies in which sublethal doses of NaClO are linked to an enhanced bacterial biofilm formation deals with E. coli ( Capita et al, 2014 ) and Staphylococcus aureus MRSA ( Buzon-Duran et al, 2017 ). The authors propose that an increase in cell surface hydrophobicity caused by NaClO is responsible for the observed induction of biofilm formation in E. coli .…”

Section: Discussionmentioning

confidence: 99%

The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

et al. 2017

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The opportunistic human pathogen Pseudomonas aeruginosa is able to survive under a variety of often harmful environmental conditions due to a multitude of intrinsic and adaptive resistance mechanisms, including biofilm formation as one important survival strategy. Here, we investigated the adaptation of P. aeruginosa PAO1 to hypochlorite (HClO), a phagocyte-derived host defense compound and frequently used disinfectant. In static biofilm assays, we observed a significant enhancement in initial cell attachment in the presence of sublethal HClO concentrations. Subsequent LC-MS analyses revealed a strong increase in cyclic-di-GMP (c-di-GMP) levels suggesting a key role of this second messenger in HClO-induced biofilm development. Using DNA microarrays, we identified a 26-fold upregulation of ORF PA3177 coding for a putative diguanylate cyclase (DGC), which catalyzes the synthesis of the second messenger c-di-GMP – an important regulator of bacterial motility, sessility and persistence. This DGC PA3177 was further characterized in more detail demonstrating its impact on P. aeruginosa motility and biofilm formation. In addition, cell culture assays attested a role for PA3177 in the response of P. aeruginosa to human phagocytes. Using a subset of different mutants, we were able to show that both Pel and Psl exopolysaccharides are effectors in the PA3177-dependent c-di-GMP network.

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Section: Discussionmentioning

confidence: 99%

The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

et al. 2017

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“…flagella and curli fimbriae), nucleic acids and lipids [ 9 , 10 ], is thought to maintain the biofilm architecture and functions, holding biofilm cells together and protecting them from environmental stresses. On these lines, it has been reported that cells in biofilms are far less sensitive to sanitizing agents than their non-attached individual planktonic counterparts [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development

et al. 2018

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Salmonella is a major food-borne pathogen able to persist in food processing environments because of its ability to form biofilms. A Salmonella enterica serotype Agona isolate from poultry (S24) was grown at 37°C in biofilms for up to 144 hours (H144) in attachment to polystyrene surfaces. Biofilm structures were examined at different stages in their development (H3, H24, H48, H72, H96 and H144) using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent dyes for live cells (SYTO 9), dead cells (propidium iodide), proteins (fluorescein isothiocyanate isomer I), lipids (DiD’oil), α-polysaccharides (concanavalin A, tetramethylrhodamine conjugate), and β-polysaccharides (calcofluor white M2R). Strain S24 developed a robust biofilm at H72 (biovolume of 166,852.5 ± 13,681.8 μm3 in the observation field of 16,078.2 μm2). The largest biovolume of live cells was also detected at H72 (128,110.3 ± 4,969.1 μm3), decreasing thereafter, which was probably owing to the detachment of cells prior to a new phase of colonization. The percentage of dead cells with regard to total cells in the biofilms increased throughout the incubation, ranging from 2.3 ± 1.1% (H24) to 44.2 ± 11.0% (H144). Proteins showed the greatest biovolume among the extracellular components within the biofilms, with values ranging from 1,295.1 ± 1,294.9 μm3 (H3) to 19,186.2 ± 8,536.0 μm3 (H96). Maximum biovolume values of 15,171.9 ± 660.7 μm3 (H48), 7,055.3 ± 4,415.2 μm3 (H144), and 2,548.6 ± 1,597.5 μm3 (H72) were observed for β-polysaccharides, α-polysaccharides and lipids, respectively. A strong (P < 0.01) positive correlation was found between the total biovolume of biofilm and the biovolume of live cells, proteins and β-polysaccharides, which may serve as useful markers of biofilm formation. The present work provides new insights into the formation of S. Agona biofilms. Our findings may contribute to the designing of reliable strategies for preventing and removing these bacterial communities.

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“…MRSA has recently attracted lot of attention in the area of food safety, as such strains may have a great impact on public health (Doulgeraki et al, 2017; Rodríguez-Lázaro et al, 2017). MRSA strains can form biofilms by adhering to different types of surfaces (e.g., indwelling medical devices) (Sadekuzzaman et al, 2015; Buzón-Durán et al, 2017). Recent studies showed that biofilms formed by S. aureus and MRSA strains may represent a hidden pathway for contamination of food and human handlers in food processing plants, by colonizing equipment and materials (Rode et al, 2007; Gutiérrez et al, 2012; Doulgeraki et al, 2017; Vergara et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Biofilms Formed by Foodborne Methicillin-Resistant Staphylococcus aureus

et al. 2018

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The objective of this study was to evaluate the capacity of 49 methicillin resistant Staphylococcus aureus (MRSA) from foods of animal origin (42 from dairy products and 7 from meat and meat products) to form biofilms. Overall, a higher biofilm biomass was observed for those MRSA strains harboring SCCmec type IV, while 8 MRSA strains (5 from dairy products and 3 from meat and meat products) were classified as strong biofilm formers in standard Tryptic Soy Broth medium. When a prolonged incubation period (48 h) was applied for those 8 MRSA strains, an increased biofilm biomass accumulation was observed during the time course, whereas the number of viable cells within the biofilms decreased as the biomass increased. The capacity of biofilm production correlated pretty well between the experiments using polystyrene microtiter plates and stainless steel micro-well plates, and significant higher values were observed in stainless steel when glucose was added to TSB during the enrichment. Biofilms were further characterized by confocal laser scanning microscope (CLSM), confirming that proteins and α-polysaccharides were the predominant components inside the extracellular polymeric matrix of biofilms formed by MRSA strains. In conclusion, our results confirm that MRSA isolates from foods of animal origin have significant capacity for forming biofilms with a high protein content, which can play a key role for the successful dissemination of MRSA lineages via food. Knowledge of the capacity of MRSA strains to produce biofilms, as well as characterization of the main MRSA biofilms matrix components, can help both to counteract the mechanisms involved in biofilm formation and resistance and to define more rational control strategies by using tailor-made cleaning agents.

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Effect of sub-inhibitory concentrations of biocides on the architecture and viability of MRSA biofilms

Cited by 50 publications

References 33 publications

The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development

Characterization of Biofilms Formed by Foodborne Methicillin-Resistant Staphylococcus aureus

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