Effect of subinhibitory concentrations of cefamandole and cefuroxime on adherence ofStaphylococcus aureus andStaphylococcus epidermidis to polystyrene culture plates

Carsenti-Etesse, H.; Durant, J.; Bernard, E; Mondain, V.; Entenza, José M.; Dellamonica, P.

doi:10.1007/bf01989980

Cited by 9 publications

(3 citation statements)

References 20 publications

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“…Enhanced biofilm formation in the presence of sub-MICs of antibiotics is a common phenomenon among bacteria and is thought to result from a global response to cell stress (7). Other studies found an inhibitory effect or no effect on S. aureus biofilm formation by sub-MICs of other cell wall-active antibiotics (cefamandole, cefuroxime, nafcillin, teicoplanin, and vancomycin) and protein synthesis inhibitors (tetracycline and roxithromycin) (5, 6, 8, 9). Most of these studies, however, utilized a single strain of S. aureus and tested antibiotics at a single or limited number of antibiotic concentrations, usually ≤1/2× MIC.…”

Section: Introductionmentioning

confidence: 97%

Low Levels of β-Lactam Antibiotics Induce Extracellular DNA Release and Biofilm Formation in Staphylococcus aureus

Kaplan

Izano

Gopal

et al. 2012

mBio

248

231

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Subminimal inhibitory concentrations of antibiotics have been shown to induce bacterial biofilm formation. Few studies have investigated antibiotic-induced biofilm formation in Staphylococcus aureus, an important human pathogen. Our goal was to measure S. aureus biofilm formation in the presence of low levels of β-lactam antibiotics. Fifteen phylogenetically diverse methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains were employed. Methicillin, ampicillin, amoxicillin, and cloxacillin were added to cultures at concentrations ranging from 0× to 1× MIC. Biofilm formation was measured in 96-well microtiter plates using a crystal violet binding assay. Autoaggregation was measured using a visual test tube settling assay. Extracellular DNA was quantitated using agarose gel electrophoresis. All four antibiotics induced biofilm formation in some strains. The amount of biofilm induction was as high as 10-fold and was inversely proportional to the amount of biofilm produced by the strain in the absence of antibiotics. MRSA strains of lineages USA300, USA400, and USA500 exhibited the highest levels of methicillin-induced biofilm induction. Biofilm formation induced by low-level methicillin was inhibited by DNase. Low-level methicillin also induced DNase-sensitive autoaggregation and extracellular DNA release. The biofilm induction phenotype was absent in a strain deficient in autolysin (atl). Our findings demonstrate that subminimal inhibitory concentrations of β-lactam antibiotics significantly induce autolysin-dependent extracellular DNA release and biofilm formation in some strains of S. aureus.

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Section: Introductionmentioning

confidence: 97%

Low Levels of β-Lactam Antibiotics Induce Extracellular DNA Release and Biofilm Formation in Staphylococcus aureus

Kaplan

Izano

Gopal

et al. 2012

mBio

248

231

View full text Add to dashboard Cite

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“…Several strategies have been proposed to inhibit biofilm formation on medical devices, including the administration of sub-MICs of antibiotics [5,12,17], use of furanone compounds [4], anti-inflammatory drugs [3], bacterial extracts [14], development of new anti-adhesive medical surfaces [8,22] and coating medical devices with several different compounds, including antibiotics [16,21,28].…”

Section: Introductionmentioning

confidence: 99%

The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm

Cerca¹,

Martins²,

Pier³

et al. 2005

Research in Microbiology

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Many studies have demonstrated that subminimal inhibitory concentrations (sub-MICs) of antibiotics can inhibit initial microbial adherence to medical device surfaces. It has been suggested that, by inhibiting initial adhesion, biofilm formation might be prevented. However, since initial adherence and subsequent biofilm formation may be two distinct phenomena, conclusions regarding the effects of sub-MIC antibiotics on initial adhesion cannot be extrapolated to biofilm formation. In this study, we evaluated the adherence of several clinical isolates of coagulase-negative staphylococci (CoNS) to acrylic and the effect of sub-MICs of vancomycin, cefazolin, dicloxacillin and combinations of these antibiotics on adherence and biofilm formation. Most of the antibiotics used resulted in effective reduction of bacterial adherence to acrylic, in some cases reaching over 70% inhibition of adherence. When strains with a high biofilm-forming capacity were grown in sub-MICs of those antibiotics, there existed combinations of the drugs that significantly inhibited biofilm formation. However, most of the antibiotic combinations that inhibited adherence did not have a profound effect on biofilm formation. When comparing the results of the effect of sub-MIC amounts of antibiotics in inhibiting adherence with their effect on the inhibition of biofilm formation, significant differences were found, mainly when using combinations of antibiotics. In general, the effect on the inhibition of adherence was greater than the effect on inhibiting biofilm formation. These results demonstrate that assays evaluating the inhibition of initial adherence to medical surfaces cannot fully predict the effect on inhibition of biofilm formation.

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“…The practical importance of these observations may be limited, since the adhesion substrates used are unlikely to be colonized by staphylococci in vivo. When po lystyrene surfaces were used as a substratum, adhesion of staphylococci was reduced by ce phalosporins, vancomycin and teicoplanin [24],…”

Section: Introductionmentioning

confidence: 99%

Antibiotics Alter Interactions of <i>Staphylococcus aureus</i> with Collagenous Substrata

Butcher¹,

Krajewska-Pietrasik

et al. 1994

Chemotherapy

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The effects of sub-minimum inhibitory concentrations (sub-MICs) of three antibiotics affecting the biosynthesis of pepti-doglycan on the interactions of Staphylococcus aureus strains with collagenous substrata were evaluated. In a system measuring binding of ¹²⁵I-labeled collagen, growth of bacteria in the presence of one-quarter MIC of cloxacillin and vancomycin reduced the number of collagen binding sites on the surface of bacteria. Growth in the presence of cefpodoxime reduced the number of collagen binding sites in one strain and increased it in another. Cefpodoxime also increased the dissociation constant of collagen binding to bacteria, 2- to 3-fold, while the other two antibiotics did not affect the affinity of the interaction. In a system measuring adhesion of ¹²⁵I-labeled bacteria to collagen-coated surfaces or cartilage, bacteria grown in the presence of cloxacillin and vancomycin attached to varying degrees depending on the strain. In contrast, compared to untreated controls as well as to bacteria treated with the other two antibiotics, growth in the presence of cefpodoxime significantly reduced adhesion of the majority of strains tested. Sub-MICs of antibiotics appear to affect staphylococcal adhesion to collagenous substrata with cefpodoxime exhibiting the strongest effect. The critical factor in reducing bacterial adhesion seems not to be the number of bacterial binding sites for collagen, but the affinity of the interaction.

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Effect of subinhibitory concentrations of cefamandole and cefuroxime on adherence ofStaphylococcus aureus andStaphylococcus epidermidis to polystyrene culture plates

Cited by 9 publications

References 20 publications

Low Levels of β-Lactam Antibiotics Induce Extracellular DNA Release and Biofilm Formation in Staphylococcus aureus

Low Levels of β-Lactam Antibiotics Induce Extracellular DNA Release and Biofilm Formation in Staphylococcus aureus

The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm

Antibiotics Alter Interactions of <i>Staphylococcus aureus</i> with Collagenous Substrata

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