Effect of submerged cultivation conditions and inducers on biosynthesis of extracellular laccase by a Trametes versicolor 1666 strain

Шахова, Н. В.; Golenkina, Sofya; Степанова, Елена В.; Loginov, Dmitry S.; Psurtseva, Nadezhda V.; Федорова, Т. В.; Королева, О. В.

doi:10.1134/s0003683811090055

Cited by 7 publications

(10 citation statements)

References 25 publications

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“…On the other hand, 0.11 µmol/L did not lead to a noticeable increase in laccase activity in C. hirsutus, C. fulvocinerea, or C. maxima (Gorbatova et al, 2006). In another study, 0.21 µmol/L syringaldazine caused a decrease in laccase activity in T. versicolor 1666 strain cultivated under submerged conditions (Shakhova et al, 2011). In the present study, syringaldazine was also tested alone or in combination with Cu for enhancing laccase production.…”

Section: Discussionmentioning

confidence: 94%

The effect of various inducers and their combinations with copper on laccaseproduction of Trametes versicolor pellets in a repeated-batch process

Birhanlı¹,

Yeşilada²

2017

Turk J Biol

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Introduction Laccases(EC 1.10.3.2, p-diphenol:dioxygen oxidoreductases) are industrially important multicoppercontaining enzymes. Although laccases are produced by some higher plants, insects, bacteria, and fungi, these enzymes are mainly abundant in white rot fungi (Gochev and Krastanov, 2007;Shraddha et al., 2011). These fungi, including Trametes, Pleurotus, Coriolopsis, and other genera, produce laccase enzymes at varying rates (Tapia-Tussell et al., 2011). Trametes is one of the most effective laccase-producing genera (Jang et al., 2002;Rosales et al., 2007). Fungal laccases are generally extracellular and they catalyze the oxidation of a wide range of compounds, such as mono-, di-, and polyphenols, aminophenols, methoxyphenols, aromatic amines, ascorbate, and nonphenolic compounds (Thurston, 1994;Couto et al., 2002;Baldrian, 2006;Patel and Gupte, 2016). The nonnecessity of any extraction process for obtaining these enzymes and their low substrate specificities make laccases suitable for many industrial applications, such as pulp delignification and biobleaching, textile dye decolorization, wastewater treatment, fruit juice processing, and construction of biosensors and biofuel cells (Minussi et al., 2002;Giardina et al., 2010;Durán et al., 2014;Yeşilada et al., 2014). Due to their broad industrial and environmental uses, these enzymes have gained increasing attention. Laccase production can be stimulated by various inductive substances, including metal ions, aromatic or phenolic compounds, alcohol, and detergents (Collins and Dobson, 1997;Birhanli and Yesilada, 2006;Tychanowicz et al., 2006;Bettin et al., 2014). Furthermore, inducer concentration, fermentation types, growth media, and incubation conditions have an important effect on laccase production and they are specific to species and strains (Boran and Yesilada, 2011). Many researchers have investigated the effects of various fermentation processes, cultivation conditions, laccase-producing organisms, and inducers on laccase production (

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Section: Discussionmentioning

confidence: 94%

The effect of various inducers and their combinations with copper on laccaseproduction of Trametes versicolor pellets in a repeated-batch process

Birhanlı¹,

Yeşilada²

2017

Turk J Biol

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“…Combined phenolic and copper inducers were indeed found to be particularly effective. [22,24,28,29] Then, the effect of VA should be further investigated in parallel with the use of vanillin as an efficient natural mediator for the degradation of lignin and pesticides. [45,48,50] Supplementing such a natural mediator to our culture medium supernatant would reduce process toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Determining the threshold for the copper concentration required to increase laccase activity would conclusively shed light on this aspect and help determining the optimal copper concentration for laccase production. [27][28][29] Low peroxidase activity was also produced in the culture medium (Fig. 3B) with a maximum activity of 0.2 U/mL.…”

Section: Trametes Versicolor Cultures With Varying Copper Concentrationsmentioning

confidence: 98%

Copper-induced Production of Laccases for Lignin Depolymerisation and Micropollutant Degradation by Laccase-mediator Systems

Pillet

Dufresne

Crelier

2021

Chimia

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Contaminants deriving from human activities represent a constantly growing threat to our environment and have a direct impact on plant and animal health. To alleviate this ecological imbalance, biocatalysis offers a green and sustainable alternative to conventional chemical processes. Due to their broad specificity, laccases are enzymes possessing excellent potential for synthetic biotransformations in various fields as well as for the degradation of organic contaminants. Herein, we produced laccases in submerged cultures of P. ostreatus and T. versicolor in three different media. The fungi/medium combination leading to the highest enzymatic activity was malt extract (2%) + yeast extract (3%) + glucose (0.8%). Laccase production was further increased by supplementing this medium with different concentrations of Cu2+, which also provided a better understanding of the induction effect. Additionally, we disclose preliminary results on the interaction of laccases with mediators (ABTS and violuric acid - VA) for two main applications: lignin depolymerisation with guaiacylglycerol-β-guaiacyl ether (GBG) as lignin model and micropollutant degradation with Remazol Brilliant Blue (RBB) as enzymatic bioremediation model. Promising results were achieved using VA to increase depolymerization of GBG dimer and to enhance RBB decolorisation.

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“…To prepare nitrogen sources for production of biosurfactants being effective for oil absorption, a solution of NaNO3 with a concentration of higher than 1 gr/lit was utilized [13]. For consumption of 1 gr/lit of crude oil the minimum of optimum amountsKH2PO4 of wereconsideredto be 0.1 gr/lit [1,9] But in upper amounts bacteria enters the logarithmic phase of rowth.…”

Section: Nutritious Elements (Nitrogen Phosphorus)mentioning

confidence: 99%

“…For motivation of native bacteria producing biosurfactants growth, the opted nutrition chemicals have to e injected to the basic culture media. Through all project stages, monitoring of pH variation and turbidity in wavelenghth of 680 nm indices is a criteria for bacteria growth and biosurfactants production [8,9]. Application of biosurfactants in raising oil recovery is one of important methods of recovering a substantial amount of leaving oil.…”

Section: Introductionmentioning

confidence: 99%

Effect of Pseudomonas Bacteria Biosurfactants on Persian Gulf Crude Oil Water Contamination: Optimized Conditions of Effective Parameters

Roozbehani¹,

Mirdrikvand²,

Moqadam³

et al. 2013

AJOCT

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In this study, effective parameters on pseudomonas bacterial culturing are analyzed individually. Pseudomonas bacteria were applied for oil pollutants segregation from a polluted water sample of Persian Gulf. The bacteria were used for cleaning the sample so that the contents' chemical features could be determined.Salinity and pH parameters were put under observation in different conditions and the results are illustrated in diagrams. Concentration of biosurfactants and conditions of culture medium like oxygen amount and temperature were all determined and analyzed. The influencing parameters on laboratory scale bacterial culturing were varied individually in order to obtain the optimum conditions for oil pollutants cleaning.Taguchi experimental design method was then applied to optimize the bacterial culturing conditions. Salinity, pH amounts, and biosurfactants concentrations were chosen as the parameters that use different levels based on Taguchi method. Eventually, a pH of 8.5, salinity of %2.5 and biosurfactants concentration of %0.1 were determined as the optimum conditions in which bacteria were cultured and grown the most.

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Effect of submerged cultivation conditions and inducers on biosynthesis of extracellular laccase by a Trametes versicolor 1666 strain

Cited by 7 publications

References 25 publications

The effect of various inducers and their combinations with copper on laccaseproduction of Trametes versicolor pellets in a repeated-batch process

The effect of various inducers and their combinations with copper on laccaseproduction of Trametes versicolor pellets in a repeated-batch process

Copper-induced Production of Laccases for Lignin Depolymerisation and Micropollutant Degradation by Laccase-mediator Systems

Effect of Pseudomonas Bacteria Biosurfactants on Persian Gulf Crude Oil Water Contamination: Optimized Conditions of Effective Parameters

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