Effect of Submicellar Concentrations of Conjugated and Unconjugated Bile Salts on the Lipid Bilayer Membrane

Abstract

The interaction of submicellar concentrations of various physiologically important unconjugated [sodium deoxycholate (NaDC), sodium cholate (NaC)] and conjugated [sodium glycodeoxycholate (NaGDC), sodium glycocholate (NaGC), sodium taurodeoxycholate (NaTDC), sodium taurocholate (NaTC)] bile salts with dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) small unilamellar vesicles in solid gel (SG) and liquid crystalline (LC) phases was investigated using the excited-state prototropism of 1-naphthol. Steady-state and time-resolved fluorescence of the two excited-state prototropic forms of 1-naphthol indicate that submicellar bile salt concentration induces hydration of the lipid bilayer membrane into the core region. This hydration effect is a general phenomenon of the bile salts studied. The bilayer hydration efficiency of the bile salt follows the order NaDC > NaC > NaGDC > NaTDC > NaGC > NaTC for both DPPC and DMPC vesicles in their SG and LC phases.

Deoxycholic acid modulates cell death signaling through changes in mitochondrial membrane properties

“…Interestingly, this effect was much less effi cient in the Chol-rich liquid-ordered phase ( l o ), possibly as a consequence of a lower affi nity of the bile acids toward more ordered membranes (14)(15)(16). This was also recently confi rmed in giant plasma membrane vesicles (GPMVs) isolated from rat tumor mast cells ( 17 ).…”

Size-Stable Solid Lipid Nanoparticles Loaded with Gd-DOTA for Magnetic Resonance Imaging

“…TDC is a bile salt and anionic detergent that is typically used to disrupt the phospholipid bilayer of a cell membrane for protein extraction 86,87 and also used for fat solubilization. 88 It has been investigated as a detergent for decreasing the size of micelles during various syntheses.…”

Effect of a glucose-triazole-hydrogenated cardanol conjugate on lipid bilayer membrane organization and thermotropic phase transition

“…3). The lowering of T m is a result of changes in acyl chain packing of lipid bilayer membrane [15][16][17]. At higher mol% (>7 mol%), the vanishing of T m in fluorescence anisotropy plot (Fig.…”

“…Amphiphilic molecules are known to interact strongly with lipid bilayer membranes due to their general similarities with lipid molecules in respect of their aqueous phase aggregation properties [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17]. Such interactions result in significant changes in the structure and dynamics of lipid bilayer membranes [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

“…Solubilisation and perturbation of lipid bilayer membranes using synthetic and biocompatible amphiphilic compounds has been used for isolation and purification of different membrane components [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. It is well known that the bioactivity and mechanistic properties of these amphiphilic compounds strongly depend on the nature of hydrophilic and hydrophobic groups [3][4][5][6][7][8][9][10][11][12][13][14]17] solubilisation of lipid bilayer membrane and thermotropic phase behaviour generally depends upon the molecular properties and concentration of the employed compound [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”