Effect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a batch bioreactor

Sinha, Jai B. P.; Bae, Jun Tae; Park, Jong Pil; Song, Chi Hyun; Yun, Jong Won

doi:10.1016/s0141-0229(01)00418-5

Cited by 55 publications

(37 citation statements)

References 15 publications

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“…This continued up to 5 days after that viscosity of the broth decreased due to the breaking stage of the pellet. These similar results were observed in fermentation broth rheology in exobiopolymer production and also for citric acid production (Sinha et al 2001). Figure 4 showed the relationship between shear stress and shear rate fitting to Bingham plastic model.…”

Section: Rheological Model Applicable To the Mycelial Broth Of Cladossupporting

confidence: 80%

“…Similar changes in morphology were reported for biopolymer production using Paecilomyces japonica in batch bioreactor. Rapid pellet formation, breakup of pellet after 7th day, and loss in rigidity were the evidence for the change in morphology (Sinha et al 2001). The hairness in the pellet increased during the initial stage, aggregated on 5th day and decreased for the remaining 2 days.…”

Section: Resultsmentioning

confidence: 93%

“…Consequently, the pellet size decreased, and filamentous mycelia were noticed in the broth. In a report, it was mentioned that the exo-biopolymer production that started slowly from the beginning of the fermentation, continually increased even after breakdown of the pellet into fragments (Sinha et al 2001). In another report, it was mentioned that the cells of P. sinclairii formed compact pellets under mild agitation during inoculum preparation but were observed to form filamentous pellet during the entire culture period and also during the exponential phase showed branched longer hyphal length, whereas in our investigation, after the breakdown of the pellet to fragments, the percentage decolorization gradually decreased and remained in constant level.…”

Section: Resultsmentioning

confidence: 99%

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Effect of transport phenomena of Cladosporium cladosporioides on decolorization and chemical oxygen demand of distillery spent wash

Rajarathinam¹

2014

Int. J. Environ. Sci. Technol.

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The focus of this report is directed towards the influence of transport properties of fermentation broth in degradation and decolorization of distillery spent wash using Cladosporium cladosporioides. Batch experiment was conducted in a bioreactor for 7 days with 250 rpm at 35°C under optimum bioprocess conditions maintained using control system. Results of the morphological analysis revealed that C. cladosporioides has been observed to form mainly pellets during fermentation under mild agitation. The organism changed from filamentous form to pellet and then breaks, ending with filamentous form. All the models Power law, Bingham plastic, and Herschel-Bulkley fitted the general trend well with high R 2 values ranging from 0.9 to 0.98, indicating shear rate thinning nature of the broth. A maximum decolorization of 72.3 % was achieved with chemical oxygen demand reduction of 74.8 % due to the low viscous and pellet nature of the organism, leading to the transport properties of the fluid. Hence, transport phenomena channalyze the metabolic path way easily and leads to a novel approach for the industries to utilize the spent wash with minimum cost.

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Section: Rheological Model Applicable To the Mycelial Broth Of Cladossupporting

confidence: 80%

Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of transport phenomena of Cladosporium cladosporioides on decolorization and chemical oxygen demand of distillery spent wash

Rajarathinam¹

2014

Int. J. Environ. Sci. Technol.

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“…The rheological behavior, for instance, is closely related to the morphology and biomass concentration, and it is exactly the broth's rheology that determines the transportation phenomena in bioreactors, which is the key to improve the bioprocess (21). On the other hand, the growth kinetics and the fungal morphology in broth fermentation are highly dependent on the culture conditions, such as carbon sources, C/N relation, initial pH and temperature, agitation intensity and aeration rate (20).…”

Section: Introductionmentioning

confidence: 99%

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Vieira¹,

Liebl²,

Tavares³

et al. 2008

Braz. J. Microbiol.

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Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence of agitation were determinant in the production of antibacterial metabolites. The characterization of the antibacterial substance by GC-MS indicated a major compound, isodrimenediol, produced by the fungus Polyporus tricholoma with activity against Staphylococcus aureus.

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“…Therefore for industrial production of these enzymes and under similar cultural conditions, involving these moulds, solid state fermentation is recommended. Substrate composition and optimization is a vital aspect of biopolymer secretion by fungi (Riley et al, 2000;Sinha et al, 2001). The use of the agro-waste in large scale fermentation will not only turn the waste into a valuable resource but also help reduce the environmental pollution due to the cellulosic biomass.…”

Section: Fungal Growth On Plant Extractmentioning

confidence: 99%

Comparative Responses of Food-Borne Aspergillus niger JX 442527 and Penicilliumminioluteum JX 442528 to Selected Plant Extracts

S.O¹,

Ganiyu²,

Ajose³

et al. 2013

GJBS

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The methanolic leaf extracts of Carica papaya, Chromolaena odorata, Alcohorneae laxiflora and Elaeis guineensis were investigated for antifungal activity against Penicillium minioluteum JX 442528 and Aspergillus niger JX 442527 in the mycology laboratory of Babcock University, Ilishan remo.. Also, common agro-wastes (wheat bran, rice bran and corn cobs) served as treatments as components of bioreaction for Penicillium minioluteum and Aspergillus niger both in submerged and solid state fermentations. Fungal growth inhibition was recorded with extracts from Carica and Chromolaena spp Extract of Elaeis guineensis extract has the lowest antifungal effect on Penicillium minioluteum. The solid state fermentation has higher enzyme activity compared to the submerged fermentation. Highest production for the 4 enzymes assayed for was observed on wheat bran media, using the SPSS statistical package. For industrial enzyme production, these cultural characteristics could therefore be critical.

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Effect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a batch bioreactor

Cited by 55 publications

References 15 publications

Effect of transport phenomena of Cladosporium cladosporioides on decolorization and chemical oxygen demand of distillery spent wash

Effect of transport phenomena of Cladosporium cladosporioides on decolorization and chemical oxygen demand of distillery spent wash

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Comparative Responses of Food-Borne Aspergillus niger JX 442527 and Penicilliumminioluteum JX 442528 to Selected Plant Extracts

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