Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

Manuhara, Yosephine Sri Wulan; Kristanti, Alfinda Novi; Utami, Edy Setiti Wida; Yachya, Arif

doi:10.1016/j.apjtb.2015.09.009

Cited by 26 publications

(30 citation statements)

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“…The same result was obtained in hairy root culture of T. paniculatum that showed the increasing of initial inoculum density can cause increasing of saponin content (Manuhara et al, 2012). According to Van Gulik et al (1994), the high of initial inoculum density can eliminated lag phase of growth curve, so specific growth rate became maximum.…”

Section: Discussionsupporting

confidence: 61%

“…Inoculums density was also reported that would significant influence on cell growth, saponin and polysaccharide production in cell culture of Panax notoginseng (Zhang and Zhong, 1997). In this study, the highest saponin content in adventitious root of T. paniculatum was achieved at aeration rate 0.75 vvm but in hairy root culture of T. paniculatum, the highest of saponin content was achieved at aeration rate 0.25 vvm (Manuhara et al, 2012). It's maybe caused by supply of oxygen in culture medium can support cell to produce secondary metabolite, so saponin content in adventitious root of T. paniculatum was increased.…”

mentioning

confidence: 66%

“…In the cell suspension culture of Elaeis guineensis Jacq. decreased of pH medium from 5.6-4 was take place very fast during 9 days culture (Gorret et al, 2004), whereas hairy root culture of Talinum paniculatum Gaertn in balloon-type bubble bioreactor at the first week pH of culture was decreased from 6-4 (Manuhara et al, 2012). Vani (1996) also reported that decreasing of pH during culture of Catharanthus roseus hairy root in the circulated bioreactor was take place at 10-15 days culture.…”

Section: Discussionmentioning

confidence: 93%

“…When aeration rate increase, produce of bubble will be increased, so it can inhibit the growth of adventitious root. It also happened in hairy root culture of T. panicultum which got the shear stress in treatment of aeration rate 0.75 vvm (Manuhara et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

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Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

Manuhara¹,

Kristanti²,

Utami³

2015

Asian J. of Biological Sciences

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Optimization of culture conditions of Talinum paniculatum Gaertn. adventitious roots in the balloon type bubble bioreactor have been done in order to increase its production of adventitious roots and saponin content. Culture conditions were used in this research were combination of aeration rate (0.25, 0.5 and 0.75 vvm) and initial inoculum density (0.5, 1, 2 g/400 mL). Bioreactor with a volume of 1000 mL was filled with 400 mL of liquid MS medium supplemented with IBA 2 mg LG 1 were then given sterile air through a microfilter (0.2 µm) with different air flow rates. Into each bioreactor were added of different inoculum density of adventitious roots that had previously been induced from leaf explants of T. paniculatum on solid MS medium supplemented with IBA 2 mg LG 1 . Cultures were maintained for 14 days and sampling was done for every two days to determine the sugar content, conductivity and pH of the medium. The results showed that the combination of aeration rate of 0.5 vvm and inoculum density of 1 g/400 mL was the best treatment that can increase biomass of adventitious roots, whereas the combination of aeration rate of 0.75 vvm and inoculum density of 2 g/400 mL was the best treatment that can be increased of saponin content.

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Section: Discussionsupporting

confidence: 61%

mentioning

confidence: 66%

Section: Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

Manuhara¹,

Kristanti²,

Utami³

2015

Asian J. of Biological Sciences

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“…According to Manuhara et al (2015) and Park et al (2011), the advantage of the hairy roots culture is often demonstrated high capacity in the production of secondary metabolites that is almost equal to or greater than the parent plants. Lee et al (2010) succeeded in obtaining alizarin of 4.3 mg/g dry weight and purpurin of 4.9 mg/g dry weight of the hairy roots cultures of Rubia akane Nakai.…”

Section: Induction Of Hairy Roots By a Rhizogenesmentioning

confidence: 99%

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

et al. 2017

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Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD 600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD 600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction. preliminary study is needed to determine the appropriate method for the hairy roots induction of gandarusa. Therefore, this study was aimed to obtain scientific information on the effect of the Agrobacterium rhizogenes strain LB510 density toward gandarusa hairyroots induction. How to CiteA protocol for hairy root induction of Justicia gendarussa Burm.f. has not been established and the present study of the influence of some factor, expecially Agrobacterium rhizogenes density, on hairy root induction in Justicia gendarussa Burm. f. was an attempt to develop a technique for hairy root production in Justicia gendarussa Burm.f. METHODS Plant preparationPlants used as explants are leaves of gandarusa (Justicia gendarussa Burm.f.). This plant was obtained from the Institute of Materia Medical, Batu, Malang, Indonesia. Leaves were taken from the second-third nodal segment. The leaves were washed under running water for ten minute and then disinfected by anti-fungiside "topsin" with 1% (v/v), followed by several rinses in sterilized distilled water and sterilized by 10% Clorox "baycline" for ten minute, followed by three times rinses in sterilized distilled water.Bacterial strain and culture media LB510 strain of Agrobacterium rhizogenes was obtained from the Research Center of Biotechnology, Indonesian Institute of Sciences (LIPI), Bogor, Indonesia. The LB510 strain was grown at room temperature (±27°C) in Luria Bertani medium (LB) (per l) (10g trypton, 5g yeast extract and 10g NaCl) of solid and liquid. Media was sterilized by autoclave at 1.2 atm 121°C for 20 minutes.The bacteria was stroke on solid media. Isolates were stored in the refrigerator for 1 week. The bacteria has been rejuvenated to each 25 mL of YMB medium and liquid LB medium. Then left at room temperature for 24 hours. Agrobacteri...

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Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Beigmohamadi

Movafeghi

Jafari

et al. 2019

Biotechnology Progress

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Plumbago europaea L. is the main source of plumbagin which is a well‐known pharmacological active compound. In this investigation, genetically transformed roots of P. europaea were obtained by improving some factors affecting the efficiency of Agrobacterium rhizoigenes‐mediated transformation such as explant type, A. rhizoigenes strain, bacterial infection period, co‐cultivation period and acetosyringone concentration. The leaf, hypocotyl and stem explants from in vitro grown plantlets were infected with bacterial strains (A4, ATCC15834, MSU440 and A13). The highest transformation rate of 69.3% was achieved after 7–9 days by inoculating A. rhizogenes MSU440 strain onto the 3‐week‐old stem explants followed by a co‐cultivation period of 2 days on a medium containing 100 μM acetosyringone. To investigate the existence of the rolB gene, polymerase chain reaction was carried out using specific primers. Effects of growth media (MS, 1/2 MS, MS‐B5 and ½ MS‐B5), different sucrose concentrations and illumination on biomass production and plumbagin biosynthesis in P. europaea hairy root cultures were analyzed using stem explants after infection with MSU440 strain. ½ MS‐B5 liquid medium containing 30 g L−1 sucrose incubated in the dark resulted in the efficient biomass production of transformed hairy roots (12.5 g fresh weight, 1.8 g dry weight) with 3.2 mg g−1 DW plumbagin accumulation. This procedure provides a framework for large‐scale cultivation of hairy roots for plumbagin production. This is the first report describing the establishment of P. europaea hairy root culture with special emphasis on plumbagin production.

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Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

Cited by 26 publications

References 12 publications

Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

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