Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.)

Karami, Omid; Deljou, Ali; Esna-Ashari, M.; Ostadahmadi, P

doi:10.1016/j.scienta.2006.07.029

Cited by 60 publications

(35 citation statements)

References 20 publications

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“…The presence of the hydrolytic enzyme "invertase" in plant cell wall and vacuole that breaks down sucrose into glucose and fructose for plant metabolism may be responsible for the superiority of sucrose over other carbon sources (Arnd and Guo-Qing, 1999). The superiority of sucrose over the other sugars employed as carbon source in this study is consistent with earlier reports for other plants such as Phoenix dactylifera (Veramendi and Navarro, 1996;Othmani et al, 2009) used as a sole carbon source and Dianthus caryophyllus (Karami et al, 2006). Baskaran and Jayabalan (2005) suggested that among the three carbon sources, sucrose proved to be better than fructose or glucose for shoot regeneration of Eclipta alba.…”

Section: Discussionsupporting

confidence: 91%

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Okafor¹,

Okezie²

2016

Afr. J. Biotechnol.

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In this study, seven carbohydrates, namely, glucose, fructose, galactose, sucrose, maltose, raffinose and starch were screened for the most suitable carbon source for the in vitro culture of oil palm (Elaeis guineensis Jacq. var. tenera) zygotic embryos on the basal media of Murashige and Skoog (MS) (1962) and Eeuwen (Y3) (1976). The embryos were excised aseptically and cultured on both media containing the different carbohydrates. Results obtained showed that while both media supported in vitro plantlet regeneration from the embryo explants, Y3 medium was significantly superior to MS medium (P=0.05) in length of root and shoot of plantlets produced. Sucrose was also significantly (P=0.05) superior to glucose and maltose while starch, raffinose, fructose and galactose had the least value in all growth parameters studied. In addition, more uniform plantlets were produced in Y3 media in which sucrose served as carbon source relative to the other six carbon sources tested. The protocol reported here has potential for speeding up germination process within a short period of time for oil palm.

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Section: Discussionsupporting

confidence: 91%

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Okafor¹,

Okezie²

2016

Afr. J. Biotechnol.

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“…A previous study demonstrated that cell identity regulators control both development and stress response pathways in response to environmental stress (Iyer-Pascuzzi et al, 2011), indicating that stress responses are diverse depending on the cell types of tissues. Because SE formation from the shoot apex can be induced by applying osmotic stress together with 2,4-D treatment in various species besides Arabidopsis, such as carnation, carrot, kidney beans and cucumber (Cabrera-Ponce et al, 2015;Ikeda-Iwai et al, 2003;Karami et al, 2006;Lou and Kako, 1995), cell identity regulators of a specific cell type in the shoot apex might regulate the molecular pathways for the response to this type of stress and for SE formation. As we observed that SEs arose from the regions of LP, we speculate that one possible cell type is LP cells and one possible cell identity regulator is DRN, which is reported to be strongly expressed in the LP of young seedlings, and whose expression was observed to expand all over the SAM region upon SE induction in our study.…”

Section: Stress and Fate Specification Of The Sementioning

confidence: 99%

Characterization of somatic embryogenesis initiated from the Arabidopsis shoot apex

Kadokura

Sugimoto

Tarr

et al. 2018

Developmental Biology

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Somatic embryogenesis is one of the best examples of the remarkable developmental plasticity of plants, in which committed somatic cells can dedifferentiate and acquire the ability to form an embryo and regenerate an entire plant. In Arabidopsis thaliana, the shoot apices of young seedlings have been reported as an alternative tissue source for somatic embryos (SEs) besides the widely studied zygotic embryos taken from siliques.Although SE induction from shoots demonstrates the plasticity of plants more clearly than the embryo-to-embryo induction system, the underlying developmental and molecular mechanisms involved are unknown. Here we characterized SE formation from shoot apex explants by establishing a system for time-lapse observation of explants during SE induction. We also established a method to distinguish SE-forming and non-SE-forming explants prior to anatomical SE formation, enabling us to identify distinct transcriptome profiles of these two explants at SE initiation. We show that embryonic fate commitment takes place at day 3 of SE induction and the SE arises directly, not through callus formation, from the base of leaf primordia just beside the shoot apical meristem (SAM), where auxin accumulates and shoot-root polarity is formed. The expression domain of a couple of key developmental genes for the SAM transiently expands at this stage. Our data demonstrate that SE-forming and 3 non-SE-forming explants share mostly the same transcripts except for a limited number of embryonic genes and root genes that might trigger the SE-initiation program. Thus, SE-forming explants possess a mixed identity (SAM, root and embryo) at the time of SE specification.

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“…Two categories of inductive conditions which allow differentiated cells to develop into competent dedifferentiated cells are now recognized, these are: plant growth regulators (PGRs) (internal and/or external cellular levels) and stress factors (osmotic shock, culture medium dehydration, water stress, heavy metal ions, alterations of culture medium pH, heat or cool shock treatments, hypoxia, antibiotics, ultraviolet radiation, and mechanical or chemical treatments) (Yu et al 2001;Ikeda-Iwai et al 2003;Aoshima, 2005;Fehér, 2005;Patnaik et al 2005;Karami et al 2006;Begun et al 2007;Potters et al 2007;Lincy et al 2009). There is ample evidence that the PGRs act as central signals to reprogram somatic cells towards embryogenic pathways (Pasternak et al 2002;Fehér et al 2003;Gaj, 2004;Fehér, 2005;Jiménez and Thomas, 2005;Thomas and Jiménez, 2005).…”

mentioning

confidence: 99%

Induction of somatic embryogenesis as an example of stress-related plant reactions

Zavattieri¹,

Frederico²,

Lima³

et al. 2010

Electro Journal of Biotech

169

110

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Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.)

Cited by 60 publications

References 20 publications

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Characterization of somatic embryogenesis initiated from the Arabidopsis shoot apex

Induction of somatic embryogenesis as an example of stress-related plant reactions

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