Effect of sucrose, exogenous product concentration, and other culture conditions on growth and steroidal alkaloid production by Solanum aviculare hairy roots

Yu, Shaoxiong; Kwok, Kian H.; Doran, Pauline M.

doi:10.1016/0141-0229(95)00057-7

Cited by 54 publications

(30 citation statements)

References 24 publications

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“…Sucrose concentration can affect hairy root growth from a metabolic substrate stance in its physical role as an osmotically active solute in the medium, as a modulator of gene expression (Lourenço et al, 2002), and as signal molecule (Weather et al, 2004). Sucrose can also affect the rate and yield of secondary metabolites (Yu et al, 1996). Sucrose requirements vary from crop to crop.…”

Section: Chemical Elicitorsmentioning

confidence: 99%

“…The carbon source used in large-scale plant tissue culture usually dominates raw material costs (Yu et al, 1996), with sucrose being the most common carbon source for growing media. Sucrose concentration can affect hairy root growth from a metabolic substrate stance in its physical role as an osmotically active solute in the medium, as a modulator of gene expression (Lourenço et al, 2002), and as signal molecule (Weather et al, 2004).…”

Section: Chemical Elicitorsmentioning

confidence: 99%

“…Sucrose can also affect the production rate and yield of secondary metabolites (Yu et al, 1996). In Artemisia annua, increasing sugar concentrations increased terpenoid metabolism (Weathers et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Optimization of culture medium is an important and indispensable step for plant tissue culture systems (Sung and Huang, 2000). The carbon source used in large-scale plant tissue culture usually dominates raw material costs (Yu et al, 1996), with sucrose being the most common carbon source.…”

Section: Introductionmentioning

confidence: 99%

“…Sucrose requirements vary from crop to crop. For optimal growth, Solanum aviculare required sucrose concentrations of 6% (Yu et al, 1996); Centurea calcitrapa required 4-6% (Lourenço et al, 2002); Datura candida × D.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Orellana¹,

Ferri²

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Section: Chemical Elicitorsmentioning

confidence: 99%

Section: Chemical Elicitorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Orellana¹,

Ferri²

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Investigation of liquid-solid hydrodynamic boundary layers and oxygen requirements in hairy root cultures

Williams

Doran

1999

Biotechnol. Bioeng.

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Rates of oxygen uptake, growth and alkaloid production by hairy roots in submerged culture were investigated using a recirculation reactor allowing operation at high liquid velocities for removal of hydrodynamic boundary layers. Measurements were performed at dissolved oxygen tensions of 31–450% air saturation. Critical oxygen concentrations for Atropa belladonna hairy roots were above air saturation, viz. 100–125% air saturation for oxygen uptake and 150% air saturation for growth, demonstrating that these roots cultivated in reactors with air sparging are oxygen‐limited. The critical oxygen tension for oxygen uptake by Solanum aviculare hairy roots was 75% air saturation. Both the specific oxygen uptake rate and specific growth rate of A. belladonna hairy roots were dependent on the mass (g dry weight) of roots present; even in the absence of boundary layers, growth did not remain exponential over the entire culture period. Cryo‐scanning electron microscopy showed that hairy roots grown submerged in liquid medium were covered with thick layers of hydrated mucilage and root hairs, representing a significant additional barrier to oxygen transfer. Roots protruding out of the liquid medium showed no evidence of mucilage accumulation. The specific oxygen demand of A. belladonna root tips was 3.3–11.5 times higher than for the remainder of the roots, the ratio increasing as the dissolved oxygen tension was reduced. Specific growth rates, biomass yields from sugar, and atropine levels were maximum at around 150% air saturation, but decreased significantly with oxygen concentrations above ca. 200%. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 729–740, 1999.

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Non-aqueous capillary electrophoresis for the simultaneous analysis of solasodine and solasonine

et al. 2000

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A capillary electrophoresis method for the simultaneous determination of the steroidal alkaloid solasodine and the derived glycoside solasonine in plant samples has been developed. Because of the low solubility of solasodine in water and in water:ethanol solutions, a non‐aqueous buffer (600 mM acetic acid, 10 mM sodium hydroxide in methanol) was utilized. The signal was measured at 200 nm and the detection limit of solasodine was 8 mg/L. The method is fast, simple and precise, and good separations were obtained with the crude plant extracts. The influence of sodium and chloride ions in the sample matrix on the performance of the method is discussed. Copyright © 2000 John Wiley & Sons, Ltd.

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Effect of sucrose, exogenous product concentration, and other culture conditions on growth and steroidal alkaloid production by Solanum aviculare hairy roots

Cited by 54 publications

References 24 publications

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Investigation of liquid-solid hydrodynamic boundary layers and oxygen requirements in hairy root cultures

Non-aqueous capillary electrophoresis for the simultaneous analysis of solasodine and solasonine

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