Effect of sucrose on sensitivity to ethylene and enzyme activities and gene expression involved in ethylene biosynthesis in cut carnations

Pun, Umed Kumar; Yamada, Tetsuya; Azuma, Mirai; Tanase, Koji; Yoshioka, Satoshi; Shimizu-Yumoto, Hiroko; Satoh, Shigeru; Ichimura, Kazuo

doi:10.1016/j.postharvbio.2016.08.001

Cited by 28 publications

(11 citation statements)

References 47 publications

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“…As expected, in the present study, the sucrose concentrations (58 or 146 mM) suppressed ethylene production in the carnation by downregulating ethylene biosynthesis genes and also maintained the flower RFW until day 6 but did not extend the vase life of the cultivar tested. However, the vase life of the sucrose-treated flowers was shorter than that of the control, which was not consistent with the studies of Pun et al (2016) and Verlinden and Garcia (2004) because they claimed that these concentrations extended vase life of carnation. In fact, sucrose serves as a substrate for respiration and cell wall synthesis and it maintains water balance, all of which should prolong vase life.…”

Section: Discussioncontrasting

confidence: 61%

Synergistic Effect of Nano-Sliver with Sucrose on Extending Vase Life of the Carnation cv. Edun

Park

Naing

et al. 2017

Front. Plant Sci.

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We investigated the effects of sucrose and nano-silver (NAg) on extending the vase life of cut carnation flowers “Edun”. Sucrose (pulse treatment) suppressed ethylene production by downregulating the genes that code for its biosynthesis. Relative to the control, however, sucrose significantly promoted xylem blockage on cut stem surfaces and reduced relative fresh weight, antioxidant activity, and cysteine proteinase inhibitor gene (DcCPi) expression. Consequently, the sucrose-treated flowers had shorter vase lives than the control. In contrast, NAg suppressed ethylene production in the petal, prevented xylem blockage in the cut stem surface, and improved all the aforementioned parameters. Therefore, NAg increased flower longevity. The most effective treatment in terms of longevity extension and parameter improvement, however, was the combination of NAg and sucrose. These results suggest that sucrose can suppress ethylene production but does not necessarily extend the vase life of the flower cultivar. The role of NAg in increasing cut carnation longevity is mainly to inhibit xylem blockage rather than suppress ethylene production, and the combined effect of NAg and sucrose is most effective at prolonging cut carnation vase life, likely due to their synergetic effects on multiple modes of action.

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Section: Discussioncontrasting

confidence: 61%

Synergistic Effect of Nano-Sliver with Sucrose on Extending Vase Life of the Carnation cv. Edun

Park

Naing

et al. 2017

Front. Plant Sci.

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“…Previous report explained that a reduction in soluble carbohydrates reduction in soluble carbohydrates. as determined by TSS content can trigger a climacteric-like production of ethylene leading to the hastening of petal senescence and vice versa (Ichimura & Suto, 1999;Pun et al, 2016). This finding agreed with the current observed variation in cut carnation percentage fresh weight loss and petal longevity ( Figure 4A-B).…”

Section: Resultssupporting

confidence: 91%

Extension of Cut Carnation Vase Life Using Compost Tea, Putrescine and Plant Extracts

Vehniwal¹,

Ofoe²,

Asiedu³

et al. 2020

SAR

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Cut flower vase life can be extended by various natural products, but their efficacy when mixed with compost tea is not reported. A study was performed to determine cut carnation (Dianthus caryophyllus cv. White Sim) vase life in 1 L of formulations (1) C3.5: 3.5% compost tea + 15 mg putrescine; (2) R5L10: C3.5 + 5 mL rosemary (Salvia rosmarinus) + 10 mL lemon (Citrus limon) extracts; (3) R10L10: C3.5 + 10 mL rosemary + 10 mL lemon extracts; (4) R5L20: C3.5 + 5 mL rosemary + 20 mL lemon extracts; (5) R10L20: C3.5 + 10 mL rosemary + 20 mL lemon extracts; (6) Chrysal: Floral Chrysal Clear (positive control); and (7) Dw: distilled water alone (negative control). The 15 mg putrescine significantly (P=0.002) reduced compost tea turbidity by 34%. The neutral pH of C3.5 and Dw did not change. However, pH increased by 24% in Chrysal and reduced by 39% in R5L10, R10L10 and R5L20 and by 54% in R10L20 on day 11. TDS and EC were highest in Chrysal from days 1 to 11. Petal discoloration, wilt, shrinkage and neck bend were delayed by C3.5 followed by Chrysal and then R5L10, but were accelerated by the other treatments. In conclusion, the cut carnation cv. White Sim longevity as influenced by variations in the vase solutions was Chrysal (14 days) > C3.5 (12.6 days) > R5L10 (10.7 days) but ≤ 9 days in the other treatments. These results were confirmed by a 2-D principal component analysis biplot. Future study will investigate microbial interaction.

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“…Previous studies had demonstrated that sugar can influence ethylene metabolism and signaling. In carnation (Dianthus caryophyllus), sucrose delayed the senescence of cut flowers by delaying ethylene biosynthesis and regulating ethylene sensitivity (Verlinden and Garcia, 2004;Hoeberichts et al, 2007;Pun et al, 2016). In Arabidopsis, glucose enhanced the degradation of the transcription factor ETHYLENE INSENSITIVE 3 (EIN3), a key regulator in ethylene signaling (Zhou et al, 1998;Shi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Auxin Regulates Sucrose Transport to Repress Petal Abscission in Rose (Rosa hybrida)

et al. 2020

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Developmental transitions in plants require adequate carbon resources, and organ abscission often occurs due to competition for carbohydrates/assimilates. Physiological studies have indicated that organ abscission may be activated by sucrose deprivation; however, an underlying regulatory mechanism that links sucrose transport to organ shedding has yet to be identified. Here, we report that transport of sucrose and the phytohormone auxin to petals through the phloem of the abscission zone (AZ) decreases during petal abscission in rose (Rosa hybrida), and that auxin regulates sucrose transport into the petals. Expression of the sucrose transporter RhSUC2 decreased in the AZ during rose petal abscission. Similarly, silencing of RhSUC2 reduced the sucrose content in the petals and promotes petal abscission. We established that the auxin signaling protein RhARF7 binds to the promoter of RhSUC2, and that silencing of RhARF7 reduces petal sucrose contents and promotes petal abscission. Overexpression of RhSUC2 in the petal AZ restored accelerated petal abscission caused by RhARF7 silencing. Moreover, treatment of rose petals with auxin and sucrose delayed ethylene-induced abscission, while silencing of RhARF7 and RhSUC2 accelerated ethylene-induced petal abscission. Our results demonstrate that auxin modulates sucrose transport during petal abscission, and that this process is regulated by a RhARF7-RhSUC2 module in the AZ.

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Effect of sucrose on sensitivity to ethylene and enzyme activities and gene expression involved in ethylene biosynthesis in cut carnations

Cited by 28 publications

References 47 publications

Synergistic Effect of Nano-Sliver with Sucrose on Extending Vase Life of the Carnation cv. Edun

Synergistic Effect of Nano-Sliver with Sucrose on Extending Vase Life of the Carnation cv. Edun

Extension of Cut Carnation Vase Life Using Compost Tea, Putrescine and Plant Extracts

Auxin Regulates Sucrose Transport to Repress Petal Abscission in Rose (Rosa hybrida)

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