Effect of Sulfonylurea Agents on Reverse Cholesterol Transport in Vitro and Vivo

Terao, Yoshio; Ayaori, Makoto; Ogura, Mariko; Yakushiji, Emi; Uto‐Kondo, Harumi; Hisada, Tetsuya; Ozasa, Hideki; Takiguchi, Shunichi; Nakaya, Kazuhiro; Sasaki, Makoto; Komatsu, Tomohiro; Iizuka, Maki; Horii, Shunpei; Mochizuki, Seibu; Yoshimura, Michihiro; Ikewaki, Katsunori

doi:10.5551/jat.7641

Cited by 21 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Oxysterols and 9-cis-retinoic acid activate transcription by binding to LXR and retinoid X receptor, respectively, to form heterodimers which, in turn, bind to conserved LXRE containing DR4 in the ABCA1 (34) and ABCG1 (16,17,20) promoter regions. We recently reported that a PPAR␥ agonist, pioglitazone, induced LXR␣ expression, leading to ABCA1/G1 transactivation, and thereby promoting cholesterol efflux to both apoA-I and HDL from macrophages (12,35). In the present study, we found that 1) astaxanthin transcriptionally induced ABCA1/G1 expression in macrophages and that 2) astaxanthin did not affect PPAR␥ or LXR levels in RAW cells (Fig.…”

Section: Discussionsupporting

confidence: 65%

“…Determination of cholesterol efflux. Cholesterol efflux experiments were performed as previously described (11)(12)(13). In brief, RAW cells or THP-1 macrophages were radiolabeled with or HDL (50 g/mL), and incubated for 24 h. The percentage of cholesterol efflux was calculated by dividing the media-derived radioactivity by the sum of the radioactivity in the media and the cells.…”

Section: Methodsmentioning

confidence: 99%

“…HDL was isolated by sequential ultracentrifugation and was prepared according to the methods previously reported (11)(12)(13).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were harvested and protein extracts were prepared as previously described (11)(12)(13). They were then subjected to Western blot analyses (10% SDS-PAGE; 25 g protein per lane) using rat anti-ABCA1 antiserum (kindly donated by Dr. S. Yokoyama of Nagoya City University) ( 15 ), and rabbit anti ABCG1 (Novus Biologicals, Littleton, CO, USA)-, LXR ␣ (PP-PPZ0412-00, Perseus Proteomics, Tokyo, Japan)-, LXR ␤ (PP-K8917-00, Perseus Proteomics)-, PPAR ␥ (sc-7196, Santa Cruz, Santa Cruz, CA, USA)-and ␤ -actin (sc-47778, Santa Cruz)-specific antibodies.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Astaxanthin Enhances ATP-Binding Cassette Transporter A1/G1 Expressions and Cholesterol Efflux from Macrophages

Iizuka

Ayaori

Uto‐Kondo

et al. 2012

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

Summary ATP-binding cassette transporters (ABC) A1 and G1 are key molecules in cholesterol efflux from macrophages, which is an initial step of reverse cholesterol transport (RCT), a major anti-atherogenic property of high-density lipoprotein (HDL). Astaxanthin is one of the naturally occurring carotenoids responsible for the pink-red pigmentation in a variety of living organisms. Although astaxanthin is known to be a strong antioxidant, it remains unclear through what mechanism of action it affects cholesterol homeostasis in macrophages. We therefore investigated the effects of astaxanthin on cholesterol efflux and ABCA1/G1 expressions in macrophages. Astaxanthin enhanced both apolipoprotein (apo) A-I-and HDL-mediated cholesterol efflux from RAW264.7 cells. In supporting these enhanced cholesterol efflux mechanisms, astaxanthin promoted ABCA1/G1 expression in various macrophages. In contrast, peroxisome proliferator-activated receptor ␥ , liver X receptor (LXR) ␣ and LXR ␤ levels remained unchanged by astaxanthin. An experiment using actinomycin D demonstrated that astaxanthin transcriptionally induced ABCA1/G1 expression, and oxysterol depletion caused by overexpression of cholesterol sulfotransferase further revealed that these inductions in ABCA1/G1 were independent of LXR-mediated pathways. Finally, we performed luciferase assays using human ABCA1/G1 promoterreporter constructs to reveal that astaxanthin activated both promoters irrespective of the presence or absence of LXR-responsive elements, indicating LXR-independence of these activations. In conclusion, astaxanthin increased ABCA1/G1 expression, thereby enhancing apoA-I/HDL-mediated cholesterol efflux from the macrophages in an LXR-independent manner. In addition to the anti-oxidative properties, the potential cardioprotective properties of astaxanthin might therefore be associated with an enhanced anti-atherogenic function of HDL.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

“…HDL was isolated by sequential ultracentrifugation and was prepared according to the methods previously reported (11)(12)(13).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Astaxanthin Enhances ATP-Binding Cassette Transporter A1/G1 Expressions and Cholesterol Efflux from Macrophages

Iizuka

Ayaori

Uto‐Kondo

et al. 2012

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Macrophage in vivo RCT studies in C57/BL6 and C57/ BL6 ApoE Ϫ / Ϫ mice Macrophage in vivo RCT studies were performed as described by Zhang et al (30)(31)(32). In brief, 12 6-to 8-week-old male C57/ BL6 mice were treated with RUT (20 mg/kg/day) or CMC-Na for 14 days.…”

Section: Histology and Immunohistochemical Stainingmentioning

confidence: 99%

Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT

Yang

Liu

et al. 2014

Journal of Lipid Research

View full text Add to dashboard Cite

Excessive accumulation of cholesterol in macrophages results in a transformation of the macrophage into foam cells and eventually causes atherosclerosis ( 1, 2 ). The pathogenic process represents a chronic and complicated interaction involving multiple factors. Reverse cholesterol transport (RCT) is a process by which extrahepatic (peripheral) cholesterol is returned to the liver for excretion in the bile and ultimately the feces, thus reducing the risk of atherosclerosis ( 3, 4 ). Although there have been great efforts in discovering drugs against atherosclerosis ( 5 ), the output has been unsatisfactory. Removal of excess cholesterol from macrophage foam cells is considered to be one of the therapeutic strategies ( 6 ). The crucial cellular transporters and receptors that relate to cholesterol effl ux include,

show abstract

Pancreatic Gland Therapies

2023

Endocrinology

View full text Add to dashboard Cite

Effect of Sulfonylurea Agents on Reverse Cholesterol Transport in Vitro and Vivo

Cited by 21 publications

References 45 publications

Astaxanthin Enhances ATP-Binding Cassette Transporter A1/G1 Expressions and Cholesterol Efflux from Macrophages

Astaxanthin Enhances ATP-Binding Cassette Transporter A1/G1 Expressions and Cholesterol Efflux from Macrophages

Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT

Pancreatic Gland Therapies

Contact Info

Product

Resources

About