Effect of sulfur and nitrogen nutrition on derepression of ferredoxin-sulfite reductase in leek seedlings

Takahashi, Shunji; Yip, Wai-Cheung; Matsugami, Toshiko R.; Tamura, Goro

doi:10.1007/bf02344550

Cited by 6 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it has been suggested that HAST (Takahashi et al 1997) and APSR (Leustek et al, 2000) may also be key regulators of pathway, activity, and we observed higher HAST expression in Houston roots. Sulfate limitation has been shown to increase SiR enzyme activity in Allium tuberosum leaf (Takahashi et al, 1996a), but not to affect SiR transcript levels in Arabidopsis (Bork et al, 1998). Similarly, we observed no effect of S treatment on SiR transcript levels, though there was evidence for higher SiR expression in CLK roots.…”

Section: Discussionsupporting

confidence: 51%

“…Cysteine synthetase catalyses the formation of cysteine from sulfide and O-acetyl serine, which is produced by serine acetyltransferase. Molecular characterization of this pathway in Allium has so far been restricted to cloning of cysteine synthase and serine acetyltransferase (Urano et al, 2000) and biochemical characterization of sulfite reductase (Takahashi et al, 1996a(Takahashi et al, , 1996b in A. tuberosum.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Sulfur Deprivation and Genotype Affect Gene Expression and Metabolism of Onion Roots

McCallum¹,

Pither-Joyce²,

Shaw³

2002

jashs

View full text Add to dashboard Cite

Genetic and environmental factors affect onion (Allium cepa L.) pungency but the molecular basis for this variation is not understood. To initiate molecular analysis of onion sulfur metabolism we isolated cDNAs from onion associated with sulfur assimilation and compared gene expression and sulfur metabolism of mild and pungent onion cultivars. We isolated cDNAs encoding homologues of 5'adenosine-phosphosulfate (APS) reductase, γ-glutamylcysteine synthetase and serine acetyl transferase using a homology-based RT-PCR approach. Homologues of high-affinity sulfate transporters and sulfite reductase were isolated from an onion root differential cDNA library enriched for genes up-regulated by 48 hours sulfur deprivation. The influence of genotype and sulfur nutrition on root expression of selected genes was measured in an experiment in which a low pungency onion cultivar (`Houston Grano') and a high pungency cultivar (`Canterbury Longkeeper') were grown hydroponically in low (0.1 meq·L-1) or high (4.0 meq·L-1) sulfate medium and harvested before bulbing. `Canterbury Longkeeper' contained higher concentrations of (+)-S-methyl-L-cysteine sulfoxide in leaf and root than `Houston Grano' but cultivars did not differ in leaf trans-(+)-S-(1-propenyl)-L-cysteine sulfoxide concentrations. `Houston Grano' accumulated significantly higher concentrations of total N, nitrate, and basic amino acids in leaves and roots, suggesting these cultivars differ markedly in maintenance of S/N homeostasis. Steady-state transcript levels of APS reductase and high-affinity sulfate transporter in roots were significantly higher (2- to 3-fold) at low sulfate. By contrast, steady state levels of ATP sulfurylase transcript were significantly higher at high sulfate levels and in `Canterbury Longkeeper'. We conclude that differences in regulation of the sulfur assimilation pathway may underlie genetic differences in pungency.

show abstract

Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 99%

Sulfur Deprivation and Genotype Affect Gene Expression and Metabolism of Onion Roots

McCallum¹,

Pither-Joyce²,

Shaw³

2002

jashs

View full text Add to dashboard Cite

show abstract

“…Decreases in APR as well as adenosine triphosphate sulfurylase were also observed in Lemna minor when plants were exposed to decreasing NO 3 -levels (Brunold and Suter, 1984). Restricted NO 3 -fertility repressed another enzyme, ferredoxin-sulfi te reductase, in the SO 4 2-reduction pathway in leek (A. tuberosum) (Takahashi et al, 1996). Sulfur and N fertility interacted to infl uence S values in Brassica napus, a high-S accumulating plant (Pinkerton, 1998).…”

mentioning

confidence: 92%

Sulfur and Nitrogen Availability Interact to Affect the Flavor Biosynthetic Pathway in Onion

Coolong¹,

Randle²

2003

jashs

View full text Add to dashboard Cite

To determine the extent to which sulfur (S) and nitrogen (N) fertility interact to influence the flavor biosynthetic pathway in onion (Allium cepa L.), `Granex 33' onions were grown in hydroponic solution culture with varying levels of S and N availability. Plants were grown at 5, 45, or 125 mg·L-1 sulfate (SO42-), and 10, 50, 90, or 130 mg·L-1 N, in a factorial combination. Total bulb S, total and individual flavor precursors and their peptide intermediates in intact onion tissue were measured. To measure the effect of S and N on alliinase activity, flavor precursors were also measured in onion macerates. Sulfur and N availability in the hydroponics solution interacted to influence all flavor compounds except S-methyl-L-cysteine sulfoxide. Levels of S-methyl-L-cysteine sulfoxide were influenced by N and S levels in the solutions; however, no interaction was present. At the lowest SO 42- or N levels, most precursors and peptides measured were present in very low concentrations. When SO 42- or N availability was adequate, differences among flavor compounds were small. Results indicated that S fertility had a greater influence on trans-S-1-propenyl-L-cysteine sulfoxide (1-PRENCSO) accumulation, while N availability had a greater influence on S-methyl-L-cysteine sulfoxide levels. Flavor precursors remaining in the onion macerates revealed that the percentage of intact precursors hydrolyzed by alliinase were not significantly influenced by either SO 42- or N levels in the solutions, except for 1-PRENCSO, which was affected by N levels. Nitrogen and S fertility interacted to influence the flavor biosynthetic pathway and may need to be considered together when manipulating onion flavor compounds.

show abstract

“…Siroheme is known to be a cofactor of two chloroplast enzymes, nitrite reductase and sulfite reductase. Synthesis of these enzymes depends on availability of nitrate and sulphate, respectively (Wray 1993, Takahashi et al. 1996).…”

Section: Introductionmentioning

confidence: 99%

Regulation of the tetrapyrrole biosynthetic pathway leading to heme and chlorophyll in plants and cyanobacteria

Vavilin

Vermaas

2002

Physiologia Plantarum

109

View full text Add to dashboard Cite

Photosynthetic organisms synthesize chlorophylls, hemes, and bilin pigments via a common tetrapyrrole biosynthetic pathway. This review summarizes current knowledge about the regulation of this pathway in plants, algae, and cyanobacteria. Particular emphasis is placed on the regulation of glutamate-1-semialdehyde formation and on the channelling of protoporphyrin IX into the heme and chlorophyll branches. The potential role of chlorophyll molecules that are not bound to photosynthetic pigment-protein complexes ('free chlorophylls') or of other Mg-containing porphyrins in regulation of tetrapyrrole synthesis is also discussed.

show abstract

Effect of sulfur and nitrogen nutrition on derepression of ferredoxin-sulfite reductase in leek seedlings

Cited by 6 publications

References 36 publications

Sulfur Deprivation and Genotype Affect Gene Expression and Metabolism of Onion Roots

Sulfur Deprivation and Genotype Affect Gene Expression and Metabolism of Onion Roots

Sulfur and Nitrogen Availability Interact to Affect the Flavor Biosynthetic Pathway in Onion

Regulation of the tetrapyrrole biosynthetic pathway leading to heme and chlorophyll in plants and cyanobacteria

Contact Info

Product

Resources

About