Effect of supplementary zinc on orthodontic tooth movement in a rat model

Abstract

Introduction: Osteoclasts and osteoblasts are responsible for regulating bone homeostasis during which the trace element zinc has been shown to exert a cumulative effect on bone mass by stimulating osteoblastic bone formation and inhibiting osteoclastic bone resorption. Objective: The aim of the present study was to investigate the effects of zinc (Zn) on orthodontic tooth movement (OTM) in a rat model. Material and Methods: A total of 44 male Wistar rats were divided into four groups of 11 animals each and received 0, 1.5, 20 and 50 ppm Zn in distilled water for 60 days. In the last 21 days of the study, nickel-titanium closed coil springs were ligated between maxillary right incisors and first molars of all rats, and tooth movement was measured at the end of this period. Histological analysis of hematoxylin/eosin slides was performed to assess root resorption lacunae, osteoclast number and periodontal ligament (PDL) width. Results: Mean OTM was calculated as 51.8, 49.1, 35.5 and 45 µm in the 0, 1.5, 20 and 50 ppm zinc-receiving groups, respectively. There were no significant differences in neither OTM nor histological parameters among the study groups (p > 0.05). Conclusion: According to the results obtained in the current investigation, increase in supplementary zinc up to 50 ppm does not affect the rate of OTM neither bone and root resorption in rats.

Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis

“…The inhibition of fracture healing by morphine was demonstrated in rats; this effect, however, was associated with inhibition of callus resorption (Chrastil et al 2013). Morphine inhibited the orthodontic movement of teeth in rats, which also indicates the inhibition of bone resorption (Akhoundi et al 2010), consistently with results of the present study (inhibition of bone resorption by morphine and buprenorphine).…”

“…The population-based and clinical studies indicate rather damaging effects of opioids on bone leading to a reduction in bone mineral density (BMD) (Pedrazzoni et al 1993; Kim et al 2006; Fortin et al 2008; Dürsteler-MacFarland et al 2011; Grey et al 2011; Duarte et al 2013) and increased risk of fracture (Guo et al 1998; Ensrud et al 2003; Vestergaard et al 2006; Saunders et al 2010; Solomon et al 2010; Miller et al 2011; Carbone et al 2013; Li et al 2013), although recently two reports indicating possible favorable opioid effects on the skeletal system in women have been published (Vestergaard et al 2012; Lee et al 2013). The unfavorable effects of opioids on the skeletal system are usually attributed to inhibitory effects on the endocrine system (hypogonadism), as well as increased tendency to falls (Vestergaard et al 2006; Daniell 2008; Saunders et al 2010; Brennan 2013; Duarte et al 2013); however, results of a small number of in vitro and in vivo experimental studies suggest that opioids may also act directly on bone tissue and exert differential effects (Hall et al 1996; Pérez-Castrillón et al 2000; King et al 2007; Akhoundi et al 2010; Bastos et al 2011; Boshra 2011; Ezzatabadipour et al 2011; Chrastil et al 2013). …”

Using Micro-Computed Tomography to Evaluate the Dynamics of Orthodontically Induced Root Resorption Repair in a Rat Model

“…The animals’ surroundings were controlled, the animal diets were closely monitored, and intensive care was provided after surgery. Non-steroidal anti-inflammatory drugs have been reported to affect the speed of tooth movement [ 14 , 15 ]. Therefore, this experiment did not use analgesics or antibiotics.…”

Alcohol Induced Osteopenia Can Cause Accelerated Orthodontic Tooth Movement in Male Wistar Rats

“…After weighing the rats, they were anesthetized by intraperitoneal injection of 0.9mg/kg of xylazine and 70mg/kg of ketamine. The technique of spring anchoring and applied force was similar to previous studies [11][12][13][14][15]. A 9-mm NiTi closed-coil spring (Hiek, 0.006 ×0.022 in, 3M Unitek, Monrovia, California, USA) was tied between the left maxillary first molars and central incisors using a 0.010" ligature wire to exert a force of 60 N. The ligature was fixed in a shallow groove which was prepared in gingival one-third of the incisor (Figure 1) with a light cure composite (Transbond XT, 3M Unitek, Monrovia, California, USA).…”