Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens

Abstract

ObjectiveThe aim of the present study was to investigate the effect of yeast with bacteriocin and Lactobacillus cultures (mixture of Lactobacillus agilis BCRC 10436 and Lactobacillus reuteri BCRC 17476) supplements, alone or in combination, on broiler chicken performance.MethodsA total of 300, 1-d-old healthy broiler chickens were randomly divided into five treatment groups: i) basal diet (control), ii) basal diet+0.25% yeast (Saccharomyces cerevisiae) (YC), iii) basal diet+0.25% yeast with bacteriocin (BA), iv) basal diet+Lactobacillus cultures (LAB), and v) basal diet +0.25% yeast with bacteriocin+Lactobacillus cultures (BA+LAB). Growth performance, cecal microbiota, cecal fermentation products, and blood biochemistry parameters were determined when chickens were 21 and 35 d old.ResultsThe supplementation of YC, BA, and BA+LAB resulted in a significantly better feed conversion rate (FCR) than that of the control group during 1 to 21 d (p<0.05). The LAB supplementation had a significant effect on the presence of Lactobacillus in the ceca at 35 d. None of the supplements had an effect on relative numbers of L. agilis and L. reuter at 21 d, but the BA supplementation resulted in the decrease of both Lactobacillus strains at 35 d. The BA+LAB supplementation resulted in higher short chain fatty acid (SCFA) in the ceca, but LAB supplementation significantly decreased the SCFA at 35 d (p<0.05). All treatments tended to decrease ammonia concentration in the ceca at 21 d, especially in the LAB treatment group. The BA supplementation alone decreased the triacylglycerol (TG) concentration significantly at 21 d (p<0.05), but the synergistic effect of BA and LAB supplementation was required to reduce the TG concentration at 35 d. The YC supplementation tended to increase the plasma cholesterol at 21 d and 35 d. However, the BA supplementation significantly decreased the cholesterol and low density lipoprotein cholesterol level at 35 d. In conclusion, the BA+LAB supplementation was beneficial to body weight gain and FCR of broiler chickens.ConclusionThe effect of BA and LAB supplementation may be a result of the growth of lactic acid bacteria enhancement and physiological characterization of bacteriocin, and it suggests that the BA and LAB supplementation level or Lactobacillus strain selection should be integrated in future supplementation designs.

Yeast fermented additive enhances broiler growth

“…Nevertheless, the supplementation of the diets with yeast probiotics at 0.15 to 0.3% have been shown to improve the performance of birds to a level achieved by the use of dietary antibiotic levels (Onifade et al, 1999). It has been recently shown that this kind of effect is due to the bacteriocin-producing effects of yeast probiotics when used single or together with lactic acid bacteria species in the diet of broiler chickens (Chen et al, 2016).…”

Effects of Bacillus subtilis, Kefir and β-Glucan Supplementation on Growth Performance, Blood Characteristics, Meat Quality and Intestine Microbiota in Broilers

“…The mechanism by which kefir affects poultry performance is largely unknown, where as, it is welles tablished that probiotics which contain Lactobacillus culture can control the pathogens population and alter gastrointestinal flora (Chen et al, 2016). These effects can increase the activity of intestinal enzymes and digestibility of nutrients (Dierck, 1989).…”

“…Lactobacillus culture into broiler feed result in an increase in feed conversion ratio (Yu et al, 2007;Chen et al, 2016). It is noteworthy that the different effects of probiotics, including relation of intestinal microflora (Yu et al, 2007;Chen et al, 2016) and immune system ).…”

“…It is noteworthy that the different effects of probiotics, including relation of intestinal microflora (Yu et al, 2007;Chen et al, 2016) and immune system ). In the current experiment, the results showed that the kefir makes suitable environment for microbial and it would be helped on the immune system.…”

Dietary or in ovo Saccharomyces cerevisiae Supplementation Developed Growth, Caecal Microbiota and Gut Histology of Broiler Chicks

“…The contradictory results on growth performance in broiler may be attributed to the fact that probiotics cannot show activity in the intestinal system of broilers. Because, Chen et al, (2017b) reported that dietary SC supplementation did not affect body weight gain, caecal lactic acid bacteria (LAB), Enterococcus, total anaerobic bacteria and short chain fatty acids. Also it was reported that dietary SC supplementation did not affect body weight gain and caecal LAB count of broilers although dietary SC supplementation increased villi length and total volatile fatty acids and decreased caecal E. Coli (Teng et al, 2017;Haldar et al, 2011).…”