Effect of Supplements with Bifidobacterium longum and Lactobacillus acidophilus on the Intestinal Microbiota during Administration of Clindamycin

Orrhage, K.; Brismar, B.; Nord, Carl Erik

doi:10.3109/08910609409141570

Cited by 77 publications

(42 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have shown (10,19,24) that clindamycin has a great effect on the anaerobic community. In one of these studies (24), alterations of the fecal microflora in 24 healthy subjects who received clindamycin for 1 week, with or without a probiotic containing two Lactobacillus strains and one Bifidobacterium strain, were monitored by cultivation.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

Jernberg

Sullivan

Edlund

et al. 2005

Appl Environ Microbiol

View full text Add to dashboard Cite

Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. During treatment, four subjects were given a probiotic, and four subjects were given a placebo. Changes in the microbial intestinal community composition and relative abundance of specific microbial populations in each subject were monitored by using viable counts and T-RFLP fingerprints. T-RFLP was also used to monitor specific bacterial populations that were either positively or negatively affected by clindamycin. Some dominant bacterial groups, such as Eubacterium spp., were easily monitored by T-RFLP, while they were hard to recover by cultivation. Furthermore, the two probiotic Lactobacillus strains were easily tracked by T-RFLP and were shown to be the dominant Lactobacillus community members in the intestinal microflora of subjects who received the probiotic.

show abstract

Section: Discussionmentioning

confidence: 99%

Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

Jernberg

Sullivan

Edlund

et al. 2005

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Several health benefits have been ascribed to bifidobacteria, including reduction of blood cholesterol (Molder, 1994), improving lactose utilization in malabsorbers (Jiang, Mustapha & Savaiano, 1996), deconjugation of bile acids (Lankaputhra & Shah, 1995) and increasing immunity in host animals (Simone et al, 1992). In addition, bifidobacteria have been shown to help maintain a healthy microbial equilibrium in the gastrointestinal tract, thereby reducing the incidence of traveler's diarrhea and antibiotic-associated diarrhea (Orrhage, Brismar & Nord, 1994). This has reinforced their status as probiotic bacteria and led to their increased use in fermented food products and pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 98%

Comparison of the sensitivity of commercial strains and infant isolates of bifidobacteria to antibiotics and bacteriocins

Kheadr

Bernoussi

Lacroix

et al. 2004

International Dairy Journal

View full text Add to dashboard Cite

“…Such 'probiotics', defined as living microorganisms, which upon ingestion in certain numbers, exert health affects beyond inherent basic nutrition (2), have been assessed in human disorders such as small bowel bacterial overgrowth (3), colorectal cancer (4), diarrhoea in children (5), traveller's diarrhoea (6) and antibiotic/ Clostridium difficile -associated diarrhoea (7,8).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of adherence of a probioticLactobacillusstrain during and afterin vivoassessment in ulcerative colitis patients

Dunne

Kelly

O'Halloran

et al. 2004

Microbial Ecology in Health and Disease

View full text Add to dashboard Cite

In a pilot-scale, open-label study to determine the ability of well-characterized probiotic Lactobacillus salivarius UCC118 cells to adhere to human epithelial cells in situ , the bacterial strain was administered to ulcerative colitis patients at approximately 10 9 CFU/day for 12 days. Microbiological analysis of biopsy specimens demonstrated that the ingested bacteria effectively adhered to both inflamed and non-inflamed mucosa of the large bowel in significant numbers. In previous reports, we have described the ability of the lactobacilli to adhere to enterocytic epithelial cells in vitro. In this study, we found that the bacteria adhered at higher levels to differentiated rather than undifferentiated epithelial monolayers; and that stationary phase lactobacilli were found to adhere to eukaryotic HT-29 and Caco-2 epithelial cells at greater levels than log phase bacterial cells. Pretreatment of the Lactobacillus cells with proteolytic enzymes abolished attachment, indicating the potential involvement of surface/exposed protein(s) as bacterial adhesin(s). SDS-PAGE (denaturing) techniques determined that the proteolytic treatment resulted in degradation of a cell wall-associated protein of approximately 84 kDa. The proteinaceous factor was purified by both anion-exchange chromatography and by gel extraction after SDS-PAGE electrophoresis, and under in vitro assay conditions proved capable of adherence and significant inhibition of bacterial attachment to enterocytic epithelial cells.

show abstract

Effect of Supplements with Bifidobacterium longum and Lactobacillus acidophilus on the Intestinal Microbiota during Administration of Clindamycin

Abstract: Thirty healthy volunteers in three groups participated in a study of

Cited by 77 publications

References 28 publications

Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

Comparison of the sensitivity of commercial strains and infant isolates of bifidobacteria to antibiotics and bacteriocins

Mechanisms of adherence of a probioticLactobacillusstrain during and afterin vivoassessment in ulcerative colitis patients

Contact Info

Product

Resources

About