Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte

Yang, Hai Li; Sun, Chongling; Sun, Cheng; Qi, Ren Li

doi:10.1007/s11033-012-1783-9

Cited by 18 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 2, ESM supplementation upregulated the gene expression level of suppressor of cytokine signaling 2 ( Socs2 ), which encodes an important negative regulator of growth hormone (GH; Flores‐Morales, Greenhalgh, Norstedt, & Rico‐Bautista, 2006). SOCS2 inhibits the GH‐induced increase in fatty acid synthase (FASN), peroxisome proliferator‐activated receptor gamma (PPARγ), carnitine palmitoyltransferase 1A (CPT1a), and acetyl‐coenzyme A carboxylase alpha (ACACA; Yang, Sun, Sun, & Qi, 2012). Hence, based on these Socs2 results, we examined the expression level of genes involved in lipid metabolism.…”

Section: Resultsmentioning

confidence: 99%

Eggshell membrane powder lowers plasma triglyceride and liver total cholesterol by modulating gut microbiota and accelerating lipid metabolism in high‐fat diet‐fed mice

Ramli

Jia

Sekine

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Obesity is a major global lifestyle disorder associated with gut microbiota. The health benefits of eggshell membrane (ESM) have been shown in previous reports, particularly as regards gut microbiota composition. Here, we investigated whether ESM improves lipid metabolism and alters gut microbiota in high‐fat diet‐fed mice. A total of 20 C57BL/6J mice aged 6 weeks were given either a control diet (CON), high‐fat diet (HFD), or high‐fat diet + 8% ESM powder (HESM) for 20 weeks. ESM supplementation in HFD‐fed mice reduced plasma triglycerides (TG) and liver total cholesterol (TC) and upregulated the expression of lipid metabolism genes carnitine palmitoyltransferase 1A and suppressor of cytokine signaling 2. Microbiota analysis showed increased relative abundance of the anti‐obesity bacterium, Lactobacillus reuteri, at 4, 12, and 16 weeks and reduced the abundance of inflammation‐related Blautia hydrogenotrophica, Roseburia faecis, and Ruminococcus callidus at 12 and 20 weeks. ESM‐supplemented mice had increased cecal isobutyrate, negatively correlated with B. hydrogenotrophica and Parabacteroides goldsteinii abundance. The results indicate that ESM supplementation in HFD‐fed mice reduced plasma TG and liver TC, possibly through alteration of lipid metabolism gene expression and gut microbiota composition, suggesting that ESM may be effective in obesity management.

show abstract

Section: Resultsmentioning

confidence: 99%

Eggshell membrane powder lowers plasma triglyceride and liver total cholesterol by modulating gut microbiota and accelerating lipid metabolism in high‐fat diet‐fed mice

Ramli

Jia

Sekine

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…Both SOCS2 and SOCS3, members of suppressor of cytokine signaling (SOCS) family, are negative regulators of the JAK/STAT signaling pathway. SOCS2 has recently been implicated in regulating fat metabolism ( 27 ). While the metabolic role of EGFR is largely unknown, EGFR was shown as a critical regulator of hepatocyte proliferation for efficient liver regeneration ( 28 ).…”

Section: Resultsmentioning

confidence: 99%

Evolution of Hepatic Steatosis to Fibrosis and Adenoma Formation in Liver-Specific Growth Hormone Receptor Knockout Mice

et al. 2014

View full text Add to dashboard Cite

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver diseases closely associated with obesity and insulin resistance; deficient growth hormone (GH) action in liver has been implicated as a mechanism. Here, we investigated the evolution of NAFLD in aged mice with liver-specific GHR deletion.Methods: We examined glucose tolerance, insulin responsiveness, and lipid profiles in aged male mice (44–50 weeks) with GHRLD. We performed proteomics analysis, pathway-based Superarray assay, as well as quantitative RT-PCR to gain molecular insight into the mechanism(s) of GHR-deficiency-mediated NAFLD. In addition, we examined the pathological changes of livers of aged GHRLD male mice.Results: The biochemical profile was consistent with that of the metabolic syndrome: abnormal glucose tolerance, impaired insulin secretion, and hyperlipidemia. RT-qPCR analysis of key markers of inflammation revealed a three- to fivefold increase in TNFα and CCL3, confirming the presence of inflammation. Expression of fibrotic markers (e.g., Col1A2 and Col3A1) was significantly increased, together with a two- to threefold increase in TGFβ transcripts. Proteomics analyses showed a marked decrease of Mup1 and Selenbp2. In addition, pathway-analysis showed that the expression of cell cycle and growth relevant genes (i.e., Ccnd1, Socs2, Socs3, and Egfr) were markedly affected in GHRLD liver. Microscopic analyses (H&E) of GHRLD livers revealed the presence of hepatic adenomas of different stages of malignancy.Conclusion: Abrogation of GH signaling in male liver leads to metabolic syndrome, hepatic steatosis, increased inflammation and fibrosis, and development of hepatic tumor. Since obesity, a common precursor of NAFLD, is a state of deficient GH secretion and action, the GHRLD model could be used to unravel the contribution of compromised hepatic GH signaling in these pathological processes, and help to identify potential targets for intervention.

show abstract

“…SOCS proteins function as classic negative regulators of JAK/STAT feedback loop downstream of cytokine signaling (Croker et al., 2003; Vlotides et al., 2004). SOCS2 overexpression has been reported to inhibit STAT3 and STAT5 activity (Yang et al., 2012). In our experiments, overexpression of SOCS2 protein did inhibit STAT5 phosphorylation but not STAT1 or STAT3 phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

IL‐8 induces miR‐424‐5p expression and modulates SOCS2/STAT5 signaling pathway in oral squamous cell carcinoma

et al. 2016

View full text Add to dashboard Cite

Suppressor of cytokine signaling (SOCS) proteins are negative feedback regulators of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway. Dysregulation of SOCS protein expression in cancers can be one of the mechanisms that maintain STAT activation, but this mechanism is still poorly understood in oral squamous cell carcinoma (OSCC). Here, we report that SOCS2 protein is significantly downregulated in OSCC patients and its levels are inversely correlated with miR‐424‐5p expression. We identified the SOCS2 protein, which modulates STAT5 activity, as a direct target of miR‐424‐5p. The miR‐424‐5p‐induced STAT5 phosphorylation, matrix metalloproteinases (MMPs) expression, and cell migration and invasion were blocked by SOCS2 restoration, suggesting that miR‐424‐5p exhibits its oncogenic activity through negatively regulating SOCS2 levels. Furthermore, miR‐424‐5p expression could be induced by the cytokine IL‐8 primarily through enhancing STAT5 transcriptional activity rather than NF‐κB signaling. Antagomir‐mediated inactivation of miR‐424‐5p prevented the IL‐8‐induced cell migration and invasion, indicating that miR‐424‐5p is required for IL‐8‐induced cellular invasiveness. Taken together, these data indicate that STAT5‐dependent expression of miR‐424‐5p plays an important role in mediating IL‐8/STAT5/SOCS2 feedback loop, and scavenging miR‐424‐5p function using antagomir may have therapeutic potential for the treatment of OSCC.

show abstract

Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte

Cited by 18 publications

References 32 publications

Eggshell membrane powder lowers plasma triglyceride and liver total cholesterol by modulating gut microbiota and accelerating lipid metabolism in high‐fat diet‐fed mice

Eggshell membrane powder lowers plasma triglyceride and liver total cholesterol by modulating gut microbiota and accelerating lipid metabolism in high‐fat diet‐fed mice

Evolution of Hepatic Steatosis to Fibrosis and Adenoma Formation in Liver-Specific Growth Hormone Receptor Knockout Mice

IL‐8 induces miR‐424‐5p expression and modulates SOCS2/STAT5 signaling pathway in oral squamous cell carcinoma

Contact Info

Product

Resources

About