Effect of Surface Modifications of Ti40Zr10Cu38Pd12 Bulk Metallic Glass and Ti-6Al-4V Alloy on Human Osteoblasts In Vitro Biocompatibility

Abstract

The use of biocompatible materials, including bulk metallic glasses (BMGs), for tissue regeneration and transplantation is increasing. The good mechanical and corrosion properties of Ti40Zr10Cu38Pd12 BMG and its previously described biocompatibility makes it a potential candidate for medical applications. However, it is known that surface properties like topography might play an important role in regulating cell adhesion, proliferation and differentiation. Thus, in the present study, Ti40Zr10Cu38Pd12 BMG and Ti6-Al-4V alloy were surface-modified electrochemically (nanomesh) or physically (microscratched) to investigate the effect of material topography on human osteoblasts cells (Saos-2) adhesion, proliferation and differentiation. For comparative purposes, the effect of mirror-like polished surfaces was also studied. Electrochemical treatments led to a highly interconnected hierarchical porous structure rich in oxides, which have been described to improve corrosion resistance, whereas microscratched surfaces showed a groove pattern with parallel trenches. Cell viability was higher than 96% for the three topographies tested and for both alloy compositions. In all cases, cells were able to adhere, proliferate and differentiate on the alloys, hence indicating that surface topography plays a minor role on these processes, although a clear cell orientation was observed on microscratched surfaces. Overall, our results provide further evidence that Ti40Zr10Cu38Pd12 BMG is an excellent candidate, in the present two topographies, for bone repair purposes.

Review on Biocompatibility and Prospect Biomedical Applications of Novel Functional Metallic Glasses

“…The TiZrCuPd alloys can also be obtained as BMGs. The Ti 40 Zr 10 Cu 38 Pd 12 metallic glass in a bulk form exhibited no statistically significant differences in cytocompatibility with Saos-2 human osteosarcoma cells ( Figure 17 b) in relation to the Ti-6Al-4V sample [ 155 ]. The similar BMG composition of Ti 40 Zr 10 Cu 36 Pd 14 was recently found to have very low ions release dominated by Ti and Cu ( Figure 18 ) which was below the cytotoxicity threshold in artificial saliva solution [ 133 ].…”

“…Moreover, the stimulation of oxides creation by laser treatment also has a beneficial influence on cell proliferation activity by improving the oxides layer capabilities to prevent the diffusion of the ions. On the contrary, the physically and electrochemically modified surface to obtain the scratch pattern and the mesh-like pattern, respectively, has no considerable influence on the proliferation, adhesion, and differentiation of cells except for directing their growth [ 155 ].…”

Effect of biomaterials hydrophobicity and roughness on biofilm development

“…Surface roughness was determined by a Leica dual core 3D microscope Leica DCM 3D (Leica Microsystems, L'Hospitalet de Llobregat, Spain) analyzing a surface of 2 mm × 1.2 mm by the Leica Map DCM 3D software [12,13]. The parameters Sa, Sq, Sz, Ssk, Sku and Str were calculated (UNE ISO, 25178-2:2013) [14].…”

On the Potential of Bulk Metallic Glasses for Dental Implantology: Case Study on Ti40Zr10Cu36Pd14

“…Even if the main properties of Ti 40 Zr 10 Cu 36 Pd 14 have already been measured in several papers [9,16,19,20,21,22,23,24], there is no complete set of data obtained on a single batch of material. For the first time, in this study, a complete characterization of the Ti 40 Zr 10 Cu 36 Pd 14 alloy was therefore performed on one unique batch of material.…”