Effect of surface potential of reverse micelle on enzyme–substrate complex formation

Ермакова, Елена; Zakhartchenko, N. L.; Zuev, Yu. F.

doi:10.1016/j.colsurfa.2007.10.027

Cited by 10 publications

(4 citation statements)

References 39 publications

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“…Accordingly, Triton X-100 increased enzyme partitioning compared with Tween-80, and SDS, which is an anionic surfactant, produced the smallest effect on enzyme partitioning. It has previously been suggested that ionic surfactant molecules bound to proteins might interrupt the tertiary structures of those proteins, and the interaction between ionic surfactants and proteins has been verified to be mediated via a combination of electrostatic and hydrophobic forces [ 24 , 25 ]. Therefore, the surfactant head group plays a determining role in protein-surfactant interactions that preferentially begin with strong ionic bond formation between the surfactant polar groups [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme fromPsidium guajavaand Recycling Phase Components

Amid

Murshid

Manap

et al. 2015

BioMed Research International

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A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme.

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Section: Resultsmentioning

confidence: 99%

A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme fromPsidium guajavaand Recycling Phase Components

Amid

Murshid

Manap

et al. 2015

BioMed Research International

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“…It is important to note that the effect of surfactants on the enzyme partitioning depends on selective chemical interactions between the enzyme molecules and surfactant. This effect is further divided into two factors which are: (i) structure of the enzyme and (ii) the chemical properties of the surfactant [30]. The results in Table 2 indicate that the selectivity and purification factors of the lipase in a non-ionic surfactant/xylitol system are significantly higher ( p < 0.05) compared to an ionic ATPS.…”

Section: Resultsmentioning

confidence: 99%

A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components

et al. 2015

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Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high OPEN ACCESSMolecules 2015, 20 11185 purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

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“…Ermakova et al [33] studied the effect of surface potential of reversed micelle on enzyme-substrate complex (encounter complex, EC) formation by Brownian dynamics simulation. The research pointed out that the negative potential of micelle increases the probability of EC formation and the positive potential decreases it.…”

Section: Electrostatic Interactionsmentioning

confidence: 99%

Effects of surfactants on enzyme-containing reversed micellar system

et al. 2011

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With the development of colloid interface and enzyme technologies, enzyme-containing reversed micellar system has been receiving much attention in bioseparation and bioconversion. Because of its high efficiency, it has brought new opportunities for the development of molecular biotechnology. Reversed micelles represent nano-sized aqueous droplets stabilized by surfactant amphiphiles inside the bulk organic solvents. The entrapped enzymes have enhanced activities under those conditions as suited in the lipid bilayers of biological membranes. The fundamentals of enzyme-containing reversed micellar system are described in this paper, with special emphasis on the effects of surfactants varying in concentrations and structures. The latest study progress on the surfactants application in enzyme-containing reversed micelles is reviewed. The introduction of novel functional surfactants in micellar enzymology and their future development are also discussed. reversed micelle, enzyme, surfactant concentration, surfactant molecular structure, enzyme-containing reversed micellar system.

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Effect of surface potential of reverse micelle on enzyme–substrate complex formation

Cited by 10 publications

References 39 publications

A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme fromPsidium guajavaand Recycling Phase Components

A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme fromPsidium guajavaand Recycling Phase Components

A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components

Effects of surfactants on enzyme-containing reversed micellar system

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