Effect of surface shear stress on the attachment of <i>pseudomonas fluorescens</i> to stainless steel under defined flow conditions

Duddridge, J.E.; Kent, Christopher; Laws, J. F.

doi:10.1002/bit.260240825

Cited by 31 publications

(38 citation statements)

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“…The fraction of very firmly adhering bacteria may increase with time. Duddridge et al (1982) showed that a certain fraction of Pseudomonas fluorescens resisted shear forces of 120 N m -~ on Perspexplates although most of the cells were removed at 11 N m2.…”

Section: Discussionmentioning

confidence: 99%

Influence of initial substratum surface tension on marine micro- and macro-fouling in the Gulf of Thailand

Becker

Siriratanachai

Hormchong

1998

Helgoländer Meeresunters.

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The density of five major groups at fouling organisms Ibacteria, diatoms, choanotlagellates, ciliates, macroorganisms) on seven artilicial substrata with surface tensions between 19.0 and 64.5 mN m ' was studied in the Gulf at Thailand. Two series of test panels r the different substrata were immersed into the sea between 3 hours and 64 days (macrotauna 128 days). The results show that surface tension has ,t limited impact on the density of the organisms. Only bacteria settled conlinuously in signilicantly lower numbers on materials within the minimum bioadheslw2 range (20-25 mN m ') than on other substrata. Signifi(:dn[ differences between the substrata may disappear after long exposure, as in series 2 after 16days For diatoms and protozoa, a colonisation pattern similar to thdt of bacteria with a minimum ol 20-25 mN m : was detected alter severdl exposure intervals, l.lowever, it was never recor(ted m more than 3 exl)osure intervals in a row. The colonisation pattern of macroorganisms could not be attributed Io substratum surface tension. An index, called "colonisation degree" is introduced to give a gene.~ral impression at the density of organisms on the materials tested. The colonisation degree did not show any signilicant difference at any exposure interval. The present results clearly suggest that substratum surface tension is easily overshadowed by other factors in colonisation processes under natural conditions.

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Section: Discussionmentioning

confidence: 99%

Influence of initial substratum surface tension on marine micro- and macro-fouling in the Gulf of Thailand

Becker

Siriratanachai

Hormchong

1998

Helgoländer Meeresunters.

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“…However, other processes are also involved and might ultimately have greater influence on biofilm formation. For example, increased levels of liquid shear have been shown to reduce bacterial adhesion (Duddridge et al, 1982;Lau and Liu, 1993;Powel and Slater, 1983;Rutter and Leech, 1980). In full-scale biofilm reactors like fluidized-bed and airlift reactors, hydrodynamic conditions differ greatly from those applied in most adhesion assays.…”

Section: Introductionmentioning

confidence: 98%

“…Biofilm formation is often studied in systems where biofilms are formed on relatively flat, fixed supports, such as flow cells, rotating disks, or tubular or annular reactors (Characklis and Marshall, 1990;Duddridge et al, 1982;Fowler, 1988;Powel and Slater, 1983;Turakhia et al, 1989). In airlift reactors, however, biofilms are formed on suspended carrier particles.…”

Section: Introductionmentioning

confidence: 99%

Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics

Gjaltema

Marel

Loosdrecht

et al. 1997

Biotechnol. Bioeng.

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“…Evidence is emerging, however, that at high fluid velocities with associated high shear rates, multispecies communities are less diverse than those developed at lower shear rates (9,23,35,42). In order to adhere to surfaces or surface-attached cells and subsequently to form biofilms, bacteria in high-velocity flowing systems must overcome shear stress at the fluid-surface interface (7,11). Attachment is facilitated by the expression of cell surface polymers that alter cell surface properties (3,15).…”

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confidence: 99%

Shear Rate Moderates Community Diversity in Freshwater Biofilms

Rickard

McBain

Stead

et al. 2004

Appl Environ Microbiol

150

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The development of freshwater multispecies biofilms at solid-liquid interfaces occurs both in quiescent waters and under conditions of high shear rates. However, the influence of hydrodynamic shear rates on bacterial biofilm diversity is poorly understood. We hypothesized that different shear rates would significantly influence biofilm diversity and alter the relative proportions of coaggregating and autoaggregating community isolates. In order to study this hypothesis, freshwater biofilms were developed at five shear rates (<0.1 to 305 S ؊1 ) in a rotating concentric cylinder reactor fed with untreated potable water. Eubacterial diversity was assessed by denaturing gradient gel electrophoresis (DGGE) and culturing on R2A agar. Fifty morphologically distinct biofilm strains and 16 planktonic strains were isolated by culturing and identified by partial 16S rRNA gene sequencing, and their relatedness was determined by the construction of a neighbor-joining phylogenetic tree. Phylogenetic and DGGE analyses showed an inverse relationship between shear rate and bacterial diversity. An in vitro aggregation assay was used to assess the relative proportions of coaggregating and autoaggregating species from each biofilm. The highest proportion of autoaggregating bacteria was present at high shear rates ( Microorganisms colonize a wide range of environments as spatially organized, taxonomically diverse, multispecies biofilm communities (1,20,44). In potable water distribution systems, many bacterial species, including members of the Proteobacteria, Actinobacteria, low-GϩC-content gram-positive bacteria, and Cytophaga-Flavobacterium-Bacterioides group, readily adhere to surfaces to form multispecies biofilms (29,41). The abilities of these taxonomically diverse organisms to attach to surfaces and codevelop within multispecies biofilms are imperative for their survival and persistence within flowing environments (4,33,44). Few published articles have described the effect of shear on the bacterial composition and diversity of freshwater biofilms. Evidence is emerging, however, that at high fluid velocities with associated high shear rates, multispecies communities are less diverse than those developed at lower shear rates (9,23,35,42). In order to adhere to surfaces or surface-attached cells and subsequently to form biofilms, bacteria in high-velocity flowing systems must overcome shear stress at the fluid-surface interface (7, 11). Attachment is facilitated by the expression of cell surface polymers that alter cell surface properties (3, 15). Properties that enhance attachment include increases in whole-cell hydrophobicity (14) and the abilities to coaggregate (25, 36) and autoaggregate (17). Recently, it was shown, using a simple recirculating tank model (35), that freshwater biofilms formed at high shear rates contained a high proportion of bacterial species that were not detectable in the surrounding fluid phase. In addition, a larger proportion of biofilm strains than of their planktonic counterparts were able to coaggr...

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Effect of surface shear stress on the attachment of pseudomonas fluorescens to stainless steel under defined flow conditions

Cited by 31 publications

References 0 publications

Influence of initial substratum surface tension on marine micro- and macro-fouling in the Gulf of Thailand

Influence of initial substratum surface tension on marine micro- and macro-fouling in the Gulf of Thailand

Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics

Shear Rate Moderates Community Diversity in Freshwater Biofilms

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