Effect of survivin-antisense oligonucleotide (ASODN) nano gene-carrier on apoptotic cycle and cyclooxygenase-2 (COX-2) expression in rectal cancer cells

Abstract

To probe effect of Survivin-ASODN nano gene-carrier on apoptotic cycle of rectal cancer cells and expression of COX-2, SW837 cells were separated into blank control (A) group, liposome transfection Survivin-ASODN (B) group, and PLA-CS nanogene transfection Survivin-ASODN (C) group. The transfection and cell apoptosis were observed under a microscope fluorescence inversion. Cell proliferation was tested by MTT method, and cell cycle was gauged by flow cytometry. Survivin and COX-2 protein expressions were detected by Western blotting. SW837 cells in group A had no fluorescent signals, while there were obvious fluorescent signals in groups B and C. Transfection rate of Survivin-ASODN in group C (52.14%) was evidently higher than group B (38.16%) which presented lower proliferation rate than group A (P <0.05) and higher proliferation rate than group C (P <0.05). From apoptosis results, the apoptosis of group B (21.59%) and group C (30.87%) was significantly increased (P <0.05) with obvious increase in group B (P <0.05). Results from apoptosis cycle showed that, the proportion of cells in groups B and C in G1 phase were more than group A (P <0.05). Protein detection results showed increased protein content of Survivin and COX-2 in group A after transfection (P <0.05). Moreover, the protein contents of Survivin and COX-2 in groups B and C were reduced compared to group A (P <0.05) after transfection with lower in group C (P <0.05). Nanocarriers can efficiently deliver Survivin-ASODN to rectal cancer cells, and effectively promote cancer cell apoptosis and reduce COX-2 expression, providing a reference method for clinical treatment of rectal cancer.