Effect of synthetic peptides on osteoblast adhesion

Dettin, Monica; Conconi, Maria Teresa; Gambaretto, Roberta; Bagno, Andrea; Bello, Carlo Di; Menti, Anna Michela; Grandi, Claudio; Parnigotto, Pier Paolo

doi:10.1016/j.biomaterials.2004.11.023

Cited by 66 publications

(77 citation statements)

References 25 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Fmoc chemistry was used. In the HVP synthesis, double coupling was used to condense the Tyr residue only, whereas in the (GRGDSP) 4 K synthesis, double couplings were applied for all the amino acid residues. Side chain protecting groups used were as follows: tBu, Tyr; Pmc, Arg; Trt, His and Asn; Boc, Lys; tBu, Ser.…”

Section: Peptide Synthesismentioning

confidence: 99%

“…Side chain protected peptides were synthesized using a Sieber Amide resin (0.22 mmoles/g for the HVP sequence and 0.25 mmoles/g for the (GRGDSP) 4 K sequence) as solid support on an automatic peptide synthesizer (Applied Biosystems, 431A). The Fmoc chemistry was used.…”

Section: Peptide Synthesismentioning

confidence: 99%

“…The first one is an evolution of method (1) that improves the strength of chemical bonds between amino groups of siloxanes, the bifunctional molecules, and the peptide (changing from imine linkages to amide ones) and allows the selective grafting of the N-terminal amino group of the peptide to the organic layer. Two peptides, promoting different osteoblast adhesion mechanisms, (GRGDSP) 4 K and HVP (reproducing the sequence 352-360 of human vitronectin (Fig. 1)), have been used to produce biomimetic surface via selective condensation to silanized titanium surface.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation

Dettin¹,

Herath²,

Gambaretto³

et al. 2008

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Bioactive molecules have been proposed to promote beneficial interactions at bone-implant interfaces for enhancing integration. The main objective of this study was to develop novel methods to functionalize oxidized titanium surfaces by the covalent immobilization of bioactive peptides, through selective reaction involving single functional groups. In the first protocol, an aminoalkylsilane was covalently linked to the Ti oxide layer, followed by covalent binding of glutaric anhydride to the free NH(2) groups. The carboxylic group of glutaric anhydride was used to condense the free N-terminal group of the side-chain protected peptide sequence. Finally, the surface was treated with trifluoroacetic acid to deprotect side-chain groups. In the second protocol, the peptide was directly anchored to the Ti oxide surface via UV activation of an arylazide peptide analogue. X-ray photoelectron spectroscopy analyses confirmed that modifications induced onto surface composition were in agreement with the reactions performed. The peptide density of each biomimetic surface was determined on the basis of radiolabeling and XPS derived reaction yields. The in vitro cellular response of the biomimetic surfaces was evaluated using a primary human osteoblast cell model. Cell adhesion, proliferation, differentiation, and mineralization were examined at initial-, short-, and long-time periods. In was shown that the biomimetic surface obtained through photoprobe-marked analogue that combines an easily-performed modification provides a favorable surface for an enhanced cellular response.

show abstract

Section: Peptide Synthesismentioning

confidence: 99%

Section: Peptide Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation

Dettin¹,

Herath²,

Gambaretto³

et al. 2008

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, studies on ß-cells cultured on various crude matrices or on their purified extracts highlighted the (17,(21)(22)(23)(24)(25)(26). Tissue engineering studies have also shown that scaffolds derived from decellularized matrices, obtained after the removal of the cellular component, support in vitro adhesion, growth, and function of several cell types (27)(28)(29) and act as a template for growth and tissue remodelling (30)(31)(32)(33)(34)(35). Based on these considerations, in this study we ascertained whether pancreas and/or liver acellular matrices support the pancreatic islet secretory function.…”

Section: Introductionmentioning

confidence: 99%

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

Carlo

Baiguera²,

Conconi³

et al. 2009

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matrix or on the liver matrix showed a progressive reduction. In order to obtain implantable devices, acellular matrix/islet cultures were entrapped into poly(vinyl alcohol) (PVA)/ poly(ethylene glycol) (PEG) tubes obtained by the freezing/thawing procedure. Under this condition, an in vitro constant insulin release was detected. The devices were then implanted into diabetic rats where reduced insulin requirement was noted suggesting insulin secretory activity of islets contained in the device. Indeed, immunofluorescence confirmed the presence of insulin-and glucagon-producing cells into the explanted devices. These data show that PVA/PEG semi-permeable membrane can obtain devices that restore, at least in part, insulin secretion.

show abstract

“…These small peptides can be produced synthetically and are easily purified, making them cost efficient compared to large proteins. Their activity is independent of their tertiary structure and they are easy to store and sterilize [42].…”

Section: Polypeptidementioning

confidence: 99%

Research Progress on the Biochemical Modification of Titanium Implant Surface

Mao¹

2014

Med chem

View full text Add to dashboard Cite

Effect of synthetic peptides on osteoblast adhesion

Cited by 66 publications

References 25 publications

Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation

Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

Research Progress on the Biochemical Modification of Titanium Implant Surface

Contact Info

Product

Resources

About