Effect of synthetic porcine gastrin-releasing peptide on plasma levels of immunoreactive cholecystokinin pancreatic polypeptide and gastrin in dogs

Inoue, Kazutomo; McKay, Daniel; Yajima, Haruaki; Rayford, Phillip L.

doi:10.1016/0196-9781(83)90106-7

Cited by 28 publications

(13 citation statements)

References 15 publications

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“…The gastric acid response to bombesin-like peptides is abolished by antrectomy (Impicciatore, Debas, Walsh, Grossman & Bertaccini, 1974) and is thus mediated by secretion of gastrin. The inhibition of acid secretion by higher doses of GRP1O was probably caused by the secretion of inhibitory peptides, such as somatostatin and cholecystokinin (Martindale, Kauffman, Levin, Walsh & Yamada, 1982;Inoue, McKay, Yajima & Rayford, 1983). The stimulatory effects of GRPI0 on pancreatic secretion may have been mediated by the release of cholecystokinin from the intestinal mucosa (Inoue et al 1983) although bombesin directly stimulates the pancreatic acinar cells to release amylase (Jensen et al 1978).…”

Section: Discussionmentioning

confidence: 99%

Metabolism and Inactivation of Gastrin Releasing Peptide by ENDOPEPTIDASE‐24.11 in the Dog

1989

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SUMMARYThe purpose of this investigation was to examine the metabolism and inactivation of gastrin releasing peptide 10 (GRP10) by endopeptidase-24. 11 prepared from the stomach wall. GRP1O was metabolized in vitro by gastric endopeptidase-24. 11. The metabolites were purified by high-pressure liquid chromatography and identified as (1-8) GRP1O and (9-10) GRP1O by amino acid analysis, indicating hydrolysis of the His8-Leu8 bond. The intravenous administration of GRP10 to conscious dogs stimulated gastrin release, gastric acid secretion, pancreatic protein secretion and pancreatic bicarbonate secretion. Incubation of GRPIO with endopeptidase-24. 11 significantly diminished the biological activity of the digests compared to control digests containing heat-inactivated enzyme. This effect was abolished by the enzyme inhibitor phosphoramidon. It is concluded that endopeptidase-24. 11 from the stomach metabolizes and inactivates GRPIO.

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Section: Discussionmentioning

confidence: 99%

Metabolism and Inactivation of Gastrin Releasing Peptide by ENDOPEPTIDASE‐24.11 in the Dog

1989

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“…There was a transient but consistent depression in the acid responses to higher doses of the peptides. Gastrin is not the only peptide released by gastrin releasing peptide, and the secretion of other peptides, such as somatostatin or cholecystokinin, may have been responsible for this depression in acid secretion (Martindale, Kauffman, Levin, Walsh & Yamada, 1982;Inoue, McKay, Yajima & Rayford, 1983).…”

Section: Discussionmentioning

confidence: 99%

Canine bombesin‐like gastrin releasing peptides stimulate gastrin release and acid secretion in the dog.

Bunnett¹,

Clark²,

Debas³

et al. 1985

The Journal of Physiology

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SUMMARY1. The synthetic mammalian bombesin-like peptides, canine gastrin releasing peptide 27, 23 and 10, and porcine gastrin releasing peptide 27 were compared with amphibian bombesin 14 and 10 during intravenous infusions into six conscious dogs with chronic gastric cannulae.2. Gastrin and gastrin releasing peptide were measured in peripherally sampled venous blood by radioimmunoassay and gastric acid secretions were collected.3. All forms of gastrin releasing peptide stimulated gastrin release and gastric acid secretion in a dose-dependent manner. The larger canine and porcine peptides were more potent than the decapeptide. Bombesin 14 was more potent than bombesin 10. 4. A rise in the venous concentration of immunoreactive gastrin releasing peptide of only 20 fmol ml-' stimulated gastrin release to about 50 % of maximal. 5. Gastrin releasing peptide 10 was cleared from the circulation three times faster than the larger forms and this may account for the apparent differences in potency.

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“…RIA of CCK: CCK was measured by a sensitive and specific RIA using the CCK antibody (UT122), whose affinity is directed mainly toward the N-terminal part of the CCK molecule [6][7][8]. There have been earlier studies validating the CCK RIA [6][7][8].…”

Section: Methodsmentioning

confidence: 99%

“…There have been earlier studies validating the CCK RIA [6][7][8]. Dupli cate determinations were calculated in pM and ex pressed as average change above basal values.…”

Section: Methodsmentioning

confidence: 99%

Effect of Vagotomy on Cholecystokinin Release and Gallbladder Contraction in Patients with Complicated Duodenal Ulcer

Huang

Huang²,

McKay³

et al. 1994

Eur Surg Res

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In this prospective study, we investigated gallbladder (GB) contraction and plasma cholecystokinin (CCK) levels in response to food intake before and 1 month after vagotomy in 27 patients with complicated duodenal ulcer. Highly selective vagotomy (HSV) was carried out in 6 patients, truncal vagotomy and pyloroplasty (TVP) in 4, truncal vagotomy and antrectomy (TVA) in 7, selective vagotomy and pyloroplasty (SVP) in 5 and selective vagotomy and antrectomy (SVA) in another 5 patients with pyloric stenosis. The results of our studies indicated that (1) basal plasma CCK levels increased significantly after vagotomy, (2) none of the vagotomy operations altered the integrated CCK response, (3) unlike HSV, SVA and TVA, SVP and TVP decreased GB emptying and (4) antrectomy significantly enhanced CCK release after food intake. We concluded from these findings that the operative procedure of HSV, SVA or TVA to circumvent bile stasis-related postvagotomy cholelithiasis is superior to SVP or TVP in the surgical treatment of complicated duodenal ulcer.

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Effect of synthetic porcine gastrin-releasing peptide on plasma levels of immunoreactive cholecystokinin pancreatic polypeptide and gastrin in dogs

Cited by 28 publications

References 15 publications

Metabolism and Inactivation of Gastrin Releasing Peptide by ENDOPEPTIDASE‐24.11 in the Dog

Metabolism and Inactivation of Gastrin Releasing Peptide by ENDOPEPTIDASE‐24.11 in the Dog

Canine bombesin‐like gastrin releasing peptides stimulate gastrin release and acid secretion in the dog.

Effect of Vagotomy on Cholecystokinin Release and Gallbladder Contraction in Patients with Complicated Duodenal Ulcer

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