Effect of Taurine and β-Alanine on Morphological Changes of Pancreas in Streptozotocin-Induced Rats

Abstract

In order to determine the effects of taurine supplementation or depletion on the morphological changes of pancreatic beta-cells in streptozotocin-induced diabetic rats, Sprague-Dawley male rats were fed the purified diets supplemented with 1, 2 or 3% taurine or 5% beta-alanine in their drinking water for 7 weeks. After 3 weeks, diabetes was induced by streptozotocin injection (50 mg/kg body-weight). Pancreatic morphology was observed by transmission electron microscopy. The pancreatic beta-cell of the non-diabetic (CO) group had the many secretory granules, rough endoplasmic reticulum and rod shaped mitochondria. However, the beta-cells of non taurine-supplemented diabetic (EO) group were severely damaged, showing depleted secretory granules. In the 1% taurine-supplemented diabetic group, the beta-cells were less damaged compared to the EO group and had some apparently normal secretory granules, but most of rough endoplasmic reticulum and mitochondria was destroyed. The beta-cell of 2% taurine-supplemented diabetic group had swollen rough endoplasmic reticulum, round-shaped mitochondria and some apparently normal secretory granules. The beta-cell of 3% taurine-supplemented diabetic group was little different from that of non-diabetic group. The pancreatic beta-cell of taurine-depleted diabetic group was not destroyed but had many small secretory granules which appeared immature. This was reflected in the blood glucose concentrations of this group. Therefore, taurine may prevent insulin-dependent diabetes by protection of the pancreatic beta-cell and may also preserve normal secretory granules. From these results, taurine supplementation may be recommended for prevention and treatment of diabetes.

Anti-Hyperglycemic, Anti-Hyperlipidemic and Antioxidant Potential of Alcoholic-Extract of Sida cordifolia (Areal Part) in Streptozotocin-Induced-Diabetes in Wistar-Rats

“…Streptozotocin solution (0.5 %) was freshly prepared in ice cold sodium citrate buffer (0.1 M; pH 4.5) in a volume of 10 mg/ml and administered to overnight fasted wistar-rats intra-peritoneally at the dose of 55 mg kg -1 body-weight [31][32][33]. The rats were kept only on glucose solution (5 %) in drinking water for next 24 h after the STZ administration to prevent hypoglycemia [34,35].…”

Effects of tephrosia purpurea aqueous seed extract on blood glucose and antioxidant enzyme activities in streptozotocin induced diabetic rats

“…Diabetes mellitus was induced in Wistar rats by single intraperitoneal injection of streptozotocin (50mg/kg bw) dissolved in 0.1M citrate buffer (pH 4.5) to overnight fasted Albino Wistar rats (Chang, 2000). The diabetes was assessed in streptozotocin induced rats by determining the blood glucose concentration, 48 hours after injection of streptozotocin.…”

Antihyperglycemic and antilipidperoxidative effects ofTephrosia purpurea seed extract in streptozotocin induced diabetic rats

“…Diabetes mellitus was induced in wistar rats by single intraperitoneal injection of streptozotocin (50mg/kg) dissolved in 0.1M-citrate buffer (pH 4.5) after overnight fasting for 12 h [16]. The diabetes was assessed by determining the blood glucose concentration within 48 hours after injection of streptozotocin.…”