Effect of taxifolin on methotrexate-induced oxidative and inflammatory oral mucositis in rats: biochemical and histopathological evaluation

Abstract

Effect of taxifolin on methotrexateinduced oxidative and inflammatory oral mucositis in rats: biochemical and histopathological evaluationThe role of oxidative stress, as well as inflammation in the pathogenesis of methotrexate (MTX)-induced oral mucositis, is a known fact. The antiinflammatory, antitumor, antimicrobial, and antioxidant properties of taxifolin-the effect we tested against MTX-induced oral mucosal damage-are well known. Objective: Evaluating biochemically and histopathologically the effects of taxifolin on methotrexate-induced oral mucosal damage in rats.Methodology: In the taxifolin+MTX (TMTX) group, 50 mg/kg taxifolin was orally administered to rats by gavage. In the MTX and healthy (HG) groups, normal saline was applied to rats as solvent by the same method. One hour after administration of taxifolin and solvent, 5 mg/kg MTX was orally administered to rats in the MTX and TMTX groups. Taxifolin and methotrexate were administered once a day for 30 days. Macroscopic, biochemical, and histopathological evaluations were performed on the inner cheek and tongue tissues of rats. These parts were removed after rats were killed with a highdose anesthesia. Results: Taxifolin with MTX prevented the increase in oxidant and pro-inflammatory parameters, such as malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), interleukin 6 (IL-6), on the inner cheek and tongue tissues of rats. Moreover, taxifolin antagonized the decrease in total glutathione (tGSH). Taxifolin decreased MTX-induced histopathological damage. Conclusion: These findings suggest that taxifolin may be useful to treat MTX-associated oral mucositis.

Ameliorative Effect of L-Carnitine against Histopathological Changes Induced by Methotrexate on the Lingual Mucosa of Albino Rats

“…31 The expression of TNF-α protein in this study was significantly increased in the rats given MTX, compared with healthy, LC, and LC+MTX groups. Bayramoglu et al, 33 and İpek et al, 28 were in accordance with the current results. It has been suggested that the signaling molecules; mitochondrial ROS plays a key role in the regulation of inflammation through the production of pro-inflammatory cytokines.…”

Methotrexate-associated oral mucositis in children with acute lymphoblastic leukemia

“…Taxifolin is a bioactive flavonoid found commonly in grapes or olive oil, among others, with well-established pharmacological effects, including having anti-inflammatory, antioxidant properties, and also antimicrobial and anticancer potential. It reduces oxidative stress, modulates signaling pathways to prevent apoptosis and decreases the expression of pro-inflammatory cytokines [58,59]. Bayramoglu et al conducted a study on MTX-treated rats, administering taxifolin by gavage.…”