Effect of Temperature and Additives on the Interaction of Ciprofloxacin Hydrochloride Drug with Polyvinylpyrrolidone and Bovine Serum Albumin: Spectroscopic and Molecular Docking Study

Abstract

The fluoroquinolone antibiotic drug namely ciprofloxacin hydrochloride (CFH) is widely prescribed for the treatment of different bacterial infections. The interaction of CFH with a synthetic polymer, polyvinyl pyrrolidone (PVP), and biopolymer, bovine serum albumin (BSA) was studied by UVvisible and fluorescence spectroscopic methods at different temperatures. The binding constant (K b ) for the CFH-PVP complex was determined from the Benesi-Hildebrand plot. PVP of different molecular weights (MW) (such as 24,000, 40,000, 360,000, and 700,000 gm. mole -1 ) were used for the interaction between CFH and PVP. There was a gradual increase in K b value and the complexation reaction was found to be much enhanced with the augmentation of the MW of PVP. The values of K b were also found to be increased with increasing temperatures as well as with the increase of electrolyte/acetic acid concentration. The Gibbs free energy of binding (∆G 0 ) values of the interaction process was negative which indicates the complex formation is thermodynamically spontaneous. The positive values of enthalpy (∆H 0 ) and entropy (∆S 0 ) of binding connote that the binding force for CFH-PVP complexation is hydrophobic in nature and the complexation is entropy controlled. The negative intrinsic enthalpy (∆H *,0 ) values indicate the high stability of CFH-PVP complexes. Molecular docking calculation discloses the existence of similar binding forces between CFH and PVP obtained by the analysis of experimental data from UV-visible spectroscopic method. The binding constant between CFH and BSA (K b ), quenching constant (K sv ), the number of binding sites (n), and the quenching rate constant (K q ) for the CFH-BSA system were also calculated. The values of K sv , K q , and n for the CFH-BSA system are lower in 0.05 mol kg -1 urea solution and higher in PVP solutions compared to those of aqueous medium.

Liquid–Liquid Equilibrium and Partitioning of Ciprofloxacin Drug in ATPSs Involve PVP10000 + Li2SO4 + Water: The Effect of Temperature and pH

“…Furthermore, the salting-out effect of salts as well as the increase of the temperature improved the binding of ciprofloxacin to PVP. 62 Our results agree well with Mukta et al 62 findings. At acidic and basic pH, the D values are more than neutral pH, which is related to the stronger interaction of the ciprofloxacin with PVP that causes a higher concentration of the drug to be transferred to the PVP-rich phase and increases the distribution coefficient (D).…”

“…The interaction between ciprofloxacin and PVP was studied by Mukta et al 62 using spectroscopic and molecular docking methods. Molecular docking studies revealed the existence of hydrogen bonding as well as the electrostatic interactions between PVP and ciprofloxacin.…”

Interactional, functional, and biological properties of lactone sophorolipid (LSL) and collagen oligopeptides (COP) in aqueous solution

“…The mixed solution was placed in a water bath to ensure a constant temperature for experiments. [ 26 ] The excitation wavelength was 290 nm, and the emission spectrum range was 280–320 nm. The excitation silt width was set to 2.5 nm and emission silt width was set to 5.0 nm.…”

“…The negative value of ΔH o also may mean the existence of weak electrostatic forces between COP and LSL. [26] 3.7 | UV-vis adsorption spectrum…”

Electrochemical and Theoretical Investigations on the Binding of Anticancer Drug Olaparib to Human Serum Albumin

“…[13][14][15] In the field of drug delivery systems, the investigation of drugprotein interactions has become prominent, leading to numerous studies. [16][17][18][19][20] Various methodologies have been used to investigate protein-drug interactions, including spectrometry, 21 fluorescence, 22 calorimetry, 23 circular dichroism (CD), 24 molecular modeling, 25 Raman spectroscopy, 26 nuclear magnetic resonance (NMR), 27 and electrochemical methods. [28][29][30][31][32] The voltametric techniques in electrochemical studies are noteworthy techniques due to its speed, efficiency, and cost-effectiveness.…”