Effect of temperature and pyrogens on single-unit activity in the rabbit's brain stem.

Cabanac, Michel; Stolwijk, JA; Hardy, James D.

doi:10.1152/jappl.1968.24.5.645

Cited by 107 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Correlation between the responsiveness of neuroses to PGE2 and temperature It has been demonstrated that the systemic injection of pyrogens decreases the activity of warm-sensitive neurones and increases the activity of cold-sensitive neurones (Cabanac, Stolwijk & Hardy, 1968;Wit & Wang, 1968) and that acetyl salicylate inhibits the pyrogen-induced neuronal activity (Wit & Wang, 1968). Since it has been proposed that warm-sensitive and cold-sensitive neurones facilitate heat defence responses and cold defence responses of thermoregulation, respectively (Boulant, Curras & Dean, 1989;Hori, 1991), these changes in POA thermosensitive neurones are appropriate in explaining the changes in thermoregulatory responses during the rising phase of fever, i.e., inhibition of heat defence responses and facilitation of cold defence responses.…”

Section: Responsiveness Of Ovlt and Poa Neurones To Pge2mentioning

confidence: 99%

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

Matsuda

Hori

Nakashima

1992

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The effects of local applications of prostaglandin E2 (PGE2) on the unit activity of fifty-one neurones in the organum vasculosum lamina terminalis (OVLT) region and fifty-eight neurones in the preoptic area (POA) were investigated in small tissue slices from the rat hypothalamus containing the OVLT and POA isolated from each other.2. Of these, thirty OVLT and twenty-eight POA neurones were warm sensitive and increased their discharge rate in response to a rise in tissue temperature. One OVLT neurone and one POA neurone were cold sensitive and showed the opposite type of responses to changes in temperature. The thermosensitivity of these neurones was still observed in a Ca21 free-high Mg2+ solution.3. Perfusion with PGE2 in doses between 1 and 250 nm changed the discharge rate in forty-two of fifty-one OVLT neurones and in thirty-two of fifty-eight POA neurones in a dose-dependent manner. The responses to PGE2 were not lost during synaptic blockade. The threshold dose of PGE2 to alter the discharge rate of the OVLT neurones (4-8+1-1 (S.E.M.) nM, n = 16) was significantly lower than that of the POA neurones (40 9 + 12 2 nm, n = 16).4. Fifteen of forty-two OVLT neurones exhibited the responses with a slower onset (latency 5-13 min) and a longer duration (20 min to 3 h), but such responses were observed in only one of thirty-two POA neurones.5. The responses of OVLT and POA neurones to PGE2 (50-250 nm) were reversibly blocked by a concurrent application of AH6809. a prostanoid EP, and/or a DP receptor antagonist.6. While there was no clear correlation between the type of thermosensitivity and the type of response to PGE2 among the POA neurones, a significantly higher incidence of inhibitory response to PGE2 was found among the warm-sensitive neurones in the OVLT region.7. The lower threshold responses to PGE2 and the higher incidence of PGE2 responsiveness among OVLT neurones are consistent with previous findings which showed that the highest density of PGE2 receptor binding and the highest pyrogenic sensitivity to microinjected PGE2 were observed in the OVLT region. The results MS 9526

show abstract

Section: Responsiveness Of Ovlt and Poa Neurones To Pge2mentioning

confidence: 99%

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

Matsuda

Hori

Nakashima

1992

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…The pathogenesis of hyperthermia associated with infection including peripheral infection, involves firstly an exogenous pyrogen such as bacterial endotoxin and secondly modification of the activities of central neurones thought to mediate thermoregulation (Cabanac, Stolwijk & Hardy, 1968;Wit & Wang, 1968;Eisenman, 1969). It is uncertain whether this central effect is exerted directly by exogenous pyrogen or indirectly by endogenous mediators such as leucocytic pyrogen (Beeson, 1948;Bennett & Beeson, 1953a, b) and/or prostaglandins of the E series (Milton & Wendlandt, 1970;Feldberg, Gupta, Milton & Wendlandt, 1973).…”

Section: Introductionmentioning

confidence: 99%

STUDY ON THE POSSIBLE ENTRY OF BACTERIAL ENDOTOXIN AND PROSTAGLANDIN E₂ INTO THE CENTRAL NERVOUS SYSTEM FROM THE BLOOD

Dascombe

Milton

1979

British J Pharmacology

View full text Add to dashboard Cite

A study has been made of the possible entry of 51Cr‐bacterial endotoxin and [5,6,8,11,12,14,15(n)‐3H]‐prostaglandin E2 ([3H]‐PGE2) into the CNS of the anaesthetized cat. No radioactivity was detected in perfusates of the preoptic‐anterior hypothalamus or in the cerebrospinal fluid (c.s.f.) in vivo, or in brain tissue post mortem following intracarotid infusion of 51Cr‐bacterial endotoxin. Intracarotid administration of [3H]‐PGE2 resulted in the entry of radioactivity into the CNS of endotoxin pretreated cats. Chromatographic analysis indicated the radioactivity in c.s.f. to be associated with PGE2 and a metabolite similar to 13, 14‐dihydro‐15‐keto PGE2. Intracarotid administration of 13, 14‐dihydro‐15‐keto [5,6,8,11,12,14(n)‐3H]‐PGE2 resulted in the presence of the compound in the CNS of the anaesthetized cat after pretreatment with bacterial endotoxin. It is concluded that PGE2 and possibly 13,14‐dihydro‐15‐keto PGE2 but not bacterial endotoxin may enter the CNS from the cerebral circulation to elicit the febrile response to bacterial endotoxin in cats.

show abstract

“…Our experiments, in which well-defined hot and cold loads and steady-state fevers were employed, lead to the same conclusion. In contrast, all experiments in which the thermosensitivity of single hypothalamic neurones has been determined have indicated that this thermosensitivity is affected by fever (Wit & Wang, 1968;Cabanac et al 1968;Eisenman, 1969;Schoener & Wang, 1975). It now seems unlikely that the conflict between the experiments on hypothalamic neuronal thermosensitivity and those employing thermal loads arises entirely from technical inadequacies.…”

Section: Discussionmentioning

confidence: 99%

“…In the other type of experiment the thermosensitivity of hypothalamic neurones has been measured. Such W. I. CRANSTON AND OTHERS experiments have all shown that pyrogens modify the response of these neurones to local temperature change (Wit & Wang, 1968;Cabanac, Stolwijk & Hardy, 1968; Eisenman, 1969;Schoener & Wang, 1975). If the thermosensitivity of the neurones contributes significantly to thermoregulation during fever, then the results of the two types of experiment are in conflict.…”

Section: Introductionmentioning

confidence: 98%

Thermoregulation in rabbits during fever.

Cranston¹,

Duff²,

Hellon³

et al. 1976

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. We have studied the effect of fever on the efficacy of the thermoregulatory control system in conscious rabbits.2. The control system was challenged by a series of systemic thermal loads produced by the intravenous infusion of hot or cold isotonic solutions. The time integral of the consequent upward or downward displacement of brain temperature was used as an index of the response of the control system. Steady-state fever was induced by intravenous infusion of plasma containing leucocyte pyrogen.3. With cold loads there was a linear relation between load and response. The regression coefficients were not significantly changed by fever in any of the six rabbits. With hot loads given to afebrile rabbits the regression ofresponse on load was generally not statistically significant, but the responses were not demonstrably greater in the febrile state. 4. We were not able to demonstrate impairment in the capacity of the febrile animal to compensate for systemic thermal loads.

show abstract

Effect of temperature and pyrogens on single-unit activity in the rabbit's brain stem.

Cited by 107 publications

References 0 publications

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

STUDY ON THE POSSIBLE ENTRY OF BACTERIAL ENDOTOXIN AND PROSTAGLANDIN E₂ INTO THE CENTRAL NERVOUS SYSTEM FROM THE BLOOD

Thermoregulation in rabbits during fever.

Contact Info

Product

Resources

About

Effect of temperature and pyrogens on single-unit activity in the rabbit's brain stem.

Cited by 107 publications

References 0 publications

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices.

STUDY ON THE POSSIBLE ENTRY OF BACTERIAL ENDOTOXIN AND PROSTAGLANDIN E2 INTO THE CENTRAL NERVOUS SYSTEM FROM THE BLOOD

Thermoregulation in rabbits during fever.

Contact Info

Product

Resources

About

STUDY ON THE POSSIBLE ENTRY OF BACTERIAL ENDOTOXIN AND PROSTAGLANDIN E₂ INTO THE CENTRAL NERVOUS SYSTEM FROM THE BLOOD