“…This fact can lead to the emergence of males and females with two resistant eggs. These resistant eggs, fertilized by males, are surrounded by a thickening dark color in the incubation chambers of adult females, called ephippium (Figure 6) [4][5][6]. Therefore, if one or more cases of ephippium occur in a culture, the neonates produced in this culture should not be used, having to discard and reassess the cultivation procedures.…”