Effect of temperature on aggressiveness of Plasmopara viticola f. sp. aestivalis and P. viticola f. sp. riparia from eastern Canada

Mouafo-Tchinda, Romaric Armel; Beaulieu, Carole; Fall, Mamadou L.; Carisse, Odile

doi:10.1080/07060661.2020.1758958

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…The manifestation of these symptoms heavily relies on the specific environmental conditions of the region, as well as the susceptibility of the host to the disease [50]. P. viticola grows optimally under high relative humidity and mild temperatures [51]. It is likely that the climatic characteristics of northern regions and associated remote islands, namely, increased humidity, UV radiation, and low average temperatures, contribute to a more active distribution of P. viticola.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Plasmopara viticola Causing Downy Mildew in Russian Far East Grapevines

Nityagovsky,

Ananev,

Suprun

et al. 2024

Horticulturae

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Downy mildew is a severe disease that leads to significant losses in grape yields worldwide. It is caused by the oomycete Plasmopara viticola. The study of the distribution of this agent and the search for endophytic organisms that inhibit the growth of P. viticola are essential objectives to facilitate the transition to sustainable and high-yield agriculture, while respecting the environment. In this study, high-throughput sequencing of the ITS (ITS1f/ITS2 region) and 16S (V4 region) amplicons was employed to analyze 80 samples of leaves and stems from different grapevine species and cultivars grown in the Russian Far East (Vitis amurensis Rupr., Vitis coignetiae Pulliat, and several grapevine cultivars). The analysis revealed the presence of P. viticola in 53.75% of the grape samples. The pathogen P. viticola was not detected in V. amurensis samples collected near Vladivostok and Russky Island. Among the P. viticola-affected samples, only two (out of the eighty analyzed grape samples) from the Makarevich vineyard in Primorsky Krai exhibited disease symptoms, while the majority appeared visually healthy. We also found six distinct P. viticola ASVs in our metagenomic data. Based on phylogenetic analysis, we hypothesize that the P. viticola population in the Russian Far East may have originated from the invasive P. viticola clade aestivalis, which has spread around the world from North America. To identify putative microbial antagonists of P. viticola, a differential analysis of high-throughput sequencing data was conducted using the DESeq2 method to compare healthy and P. viticola-affected samples. The in silico analysis revealed an increased representation of certain taxa in healthy samples compared to P. viticola-affected ones: fungi—Kabatina sp., Aureobasidium sp., and Vishniacozyma sp.; bacteria—Hymenobacter spp., Sphingomonas spp., Massilia spp., Methylobacterium-Methylorubrum spp., and Chryseobacterium spp. This in-silico-obtained information on the potential microbial antagonists of P. viticola serves as a theoretical basis for the development of biocontrol agents for grapevine downy mildew.

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Section: Discussionmentioning

confidence: 99%

Distribution of Plasmopara viticola Causing Downy Mildew in Russian Far East Grapevines

Nityagovsky,

Ananev,

Suprun

et al. 2024

Horticulturae

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“…During this time, P. viticola produces intracellular haustoria (Figure 1), with which it extracts nutrients from the host plant [25,26]. If optimal conditions of high humidity and temperatures between 15 • C and 25 • C exist, the intercellular mycelia will begin to produce sporangiophores (Figure 1) approximately one week after the initial infection occurred [25,27,28]. During the period from infection to the production of sporangiophores, no symptoms will be visible.…”

Section: Pathogen Life Cyclementioning

confidence: 99%

“…There is also evidence to suggest that the nighttime temperature can affect the progression of the disease, with warmer temperatures (25 • C) resulting in accelerated growth and sporulation compared to cooler temperatures (15 • C) [29]. In a controlled study of two P. viticola cryptic species, temperature was observed to have a significant effect on the aggressiveness of the pathogen, and aggressiveness differed between the species evaluated [28]. Thus, when warm temperatures and high humidity are sustained, the infection cycle can progress more rapidly and will be compounded by the presence of both sexual and asexual spores.…”

Section: Pathogen Life Cyclementioning

confidence: 99%

Traditional and Emerging Approaches for Disease Management of Plasmopara viticola, Causal Agent of Downy Mildew of Grape

Clippinger,

Dobry,

Laffan

et al. 2024

Agriculture

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The oomycete Plasmopara viticola, which causes downy mildew, is currently one of the most destructive pathogens affecting grape production. Although native to the eastern United States, P. viticola was introduced into Europe in the mid-to-late 1800s and is now found in virtually every grape-growing region of the world. Since its discovery, much effort has been made to understand the life cycle and infection process of the pathogen to develop more effective management practices. Widespread application of fungicides, especially those which have only one mode of action, has led to an increased occurrence of resistance to these treatments. Thus, with increased fungicide resistance and rising environmental concerns surrounding their use, traditional chemical management practices have begun to fall out of favor. Newer approaches, from targeted breeding utilizing quantitative trait loci to biological control agents, are continually being investigated and adapted to limit the damage caused by downy mildew. This review summarizes the current knowledge of the pathogen and methods of its control and explores potential avenues for future research focused on hypovirulence and biological control agents.

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“…are obligate, biotrophic and polycyclic pathogens, affecting all green organs of their host (Gessler et al, 2011 ; Gadoury et al, 2012 ). Plasmopara viticola grows optimally under high relative humidity (RH) and mild temperatures (Caffi et al, 2013 ; Mouafo-Tchinda et al, 2021 ), while E. necator has an optimal growth at 85% RH and 26°C (Carroll and Wilcox, 2003 ). Under ideal conditions, pathogens develop several cycles of clonal reproduction, causing severe damage to fruit quality and yield.…”

Section: Introductionmentioning

confidence: 99%

Phenotyping for QTL identification: A case study of resistance to Plasmopara viticola and Erysiphe necator in grapevine

Possamai

Wiedemann‐Merdinoglu

2022

Front. Plant Sci.

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Vitis vinifera is the most widely cultivated grapevine species. It is highly susceptible to Plasmopara viticola and Erysiphe necator, the causal agents of downy mildew (DM) and powdery mildew (PM), respectively. Current strategies to control DM and PM mainly rely on agrochemical applications that are potentially harmful to humans and the environment. Breeding for resistance to DM and PM in wine grape cultivars by introgressing resistance loci from wild Vitis spp. is a complementary and more sustainable solution to manage these two diseases. During the last two decades, 33 loci of resistance to P. viticola (Rpv) and 15 loci of resistance to E. necator (Ren and Run) have been identified. Phenotyping is salient for QTL characterization and understanding the genetic basis of resistant traits. However, phenotyping remains a major bottleneck for research on Rpv and Ren/Run loci and disease resistance evaluation. A thorough analysis of the literature on phenotyping methods used for DM and PM resistance evaluation highlighted phenotyping performed in the vineyard, greenhouse or laboratory with major sources of variation, such as environmental conditions, plant material (organ physiology and age), pathogen inoculum (genetic and origin), pathogen inoculation (natural or controlled), and disease assessment method (date, frequency, and method of scoring). All these factors affect resistance assessment and the quality of phenotyping data. We argue that the use of new technologies for disease symptom assessment, and the production and adoption of standardized experimental guidelines should enhance the accuracy and reliability of phenotyping data. This should contribute to a better replicability of resistance evaluation outputs, facilitate QTL identification, and contribute to streamline disease resistance breeding programs.

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Effect of temperature on aggressiveness of Plasmopara viticola f. sp. aestivalis and P. viticola f. sp. riparia from eastern Canada

Cited by 7 publications

References 23 publications

Distribution of Plasmopara viticola Causing Downy Mildew in Russian Far East Grapevines

Distribution of Plasmopara viticola Causing Downy Mildew in Russian Far East Grapevines

Traditional and Emerging Approaches for Disease Management of Plasmopara viticola, Causal Agent of Downy Mildew of Grape

Phenotyping for QTL identification: A case study of resistance to Plasmopara viticola and Erysiphe necator in grapevine

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