Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments

Paksanont, Suporn; Sintiprungrat, Kitisak; Yimthin, Thatcha; Pumirat, Pornpan; Peacock, Sharon J.; Chantratita, Narisara

doi:10.1038/s41598-018-27356-7

Cited by 29 publications

(25 citation statements)

References 45 publications

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“…In B. seminalis TC3.4.2R3, the flagellin gene fliC was downregulated, while the flhD , flhC and fliL genes were upregulated at 37 °C, suggesting differential regulation of these genes. Similar results were found in B. pseudomallei strain 153: more motility was observed at 37 °C than at 25 °C and at 30 °C more so than at 42 °C, although fliC was downregulated (−2.60-fold) 40 .…”

Section: Discussionsupporting

confidence: 83%

“…Since cytochrome genes are induced, it is expected that a microaerobic environment is created because cytochromes have high affinity for oxygen, triggering oxygen depletion in Burkholderia 41 . For B. seminalis TC3.4.2R3 grown at 37 °C, genes encoding chaperons and heat-shock proteins, universal stress proteins and several genes involved in cellular detoxification were upregulated to protect and ensure cell survival, as also observed in B. pseudomallei strain 153 at increased temperature 40 .…”

Section: Discussionmentioning

confidence: 65%

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Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Gonçalves

Denman

Ferreira

et al. 2019

Sci Rep

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Burkholderia seminalis strain TC3.4.2R3 is an endophytic bacterium isolated from sugarcane roots that produces antimicrobial compounds, facilitating its ability to act as a biocontrol agent against phytopathogenic bacteria. In this study, we investigated the thermoregulation of B. seminalis TC3.4.2R3 at 28 °C (environmental stimulus) and 37 °C (host-associated stimulus) at the transcriptional and phenotypic levels. The production of biofilms and exopolysaccharides such as capsular polysaccharides and the biocontrol of phytopathogenic fungi were enhanced at 28 °C. At 37 °C, several metabolic pathways were activated, particularly those implicated in energy production, stress responses and the biosynthesis of transporters. Motility, growth and virulence in the Galleria mellonella larvae infection model were more significant at 37 °C. Our data suggest that the regulation of capsule expression could be important in virulence against G. mellonella larvae at 37 °C. In contrast, B. seminalis TC3.4.2R3 failed to cause death in infected BALB/c mice, even at an infective dose of 10 7 CFU.mL −1 . We conclude that temperature drives the regulation of gene expression in B. seminalis during its interactions with the environment.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 65%

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Gonçalves

Denman

Ferreira

et al. 2019

Sci Rep

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“…Our recent study demonstrated that the human body temperature at 37°C facilitates a high growth rate, flagellin gene ( fliC ) expression and maximum motility functions in B . pseudomallei [ 30 ]. These mechanisms and the abundant flagella production may be a prerequisite for acute infection and bacterial dissemination.…”

Section: Discussionmentioning

confidence: 99%

Immune response to recombinant Burkholderia pseudomallei FliC

et al. 2018

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Burkholderia pseudomallei is a flagellated Gram-negative bacterium which is the causative agent of melioidosis. The disease poses a major public health problem in tropical regions and diabetes is a major risk factor. The high mortality rate of melioidosis is associated with severe sepsis which involves the overwhelming production of pro-inflammatory cytokines. Bacterial flagellar protein (flagellin) activates Toll-like receptor 5 (TLR5)-mediated innate immune signaling pathways and induces adaptive immune response. However, previous studies of TLR5 signaling in melioidosis have been performed using recombinant flagellin from Salmonella Typhimurium instead of B. pseudomallei. This study aimed to investigate human innate immune response and antibody response against a recombinant B. pseudomallei flagellin (rFliC). We prepared B. pseudomallei rFliC and used it to stimulate HEK-BlueTM-hTLR5 and THP1-DualTM cells to assess TLR5 activation. Subsequently, whole blood stimulation assays with rFliC were performed ex vivo. TLR5-flagellin interactions trigger activation of transcription factor NF-κB in HEK-BlueTM-hTLR5 cells. Pro-inflammatory cytokine (IL-1β, IL-6, and TNF-α) productions from whole blood in response to rFliC differed between fourteen healthy individuals. The levels of these cytokines changed in a dose and time-dependent manner. ELISA was used to determine rFliC-specific antibodies in serum samples from different groups of melioidosis patients and healthy subjects. IgG antibody to rFliC in melioidosis patients with diabetes were higher compared with non-diabetic patients. Our results show that B. pseudomallei flagellin is a potent immune stimulator and that the immune responses to rFliC are different among individuals. This may provide valuable insights toward the potential use of rFliC in vaccine development.

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“…In this study we excluded rice fields with a history of extensive use of fertilizers or chemicals, but none of the fields were completely free of fertilizer use. Several reports have suggested that a wide range of environmental factors might influence the distribution of B. pseudomallei [49,50,51,52]. Only gross soil texture and altitude were measured in this study.…”

Section: Discussionmentioning

confidence: 99%

Presence of Burkholderia pseudomallei in the ‘Granary of Myanmar’

Win¹,

Su²,

Than³

et al. 2019

TropicalMed

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Melioidosis is a frequently fatal infectious disease caused by the Gram negative bacillus Burkholderia pseudomallei. Although it was originally discovered in Myanmar, the disease disappeared from sight for many decades. This study focuses on detection of B. pseudomallei in soil in selected sampling sites in an attempt to start to fill the gaps in the current status of our knowledge of the geographical distribution of B. pseudomallei in soil in Myanmar. This cross-sectional study consists of 400 soil samples from 10 selected study townships from two major paddy growing regions. Bacterial isolation was done using a simplified method for the isolation of Burkholderia pseudomallei from soil. In this study, only 1% (4/400) of soil samples were found to be positive; two of four were found at 90 cm depth and another two positive samples were found at 30 cm and 60 cm. This survey has confirmed the presence of environmental B. pseudomallei in Myanmar indicating that the conditions are in place for melioidosis acquisition.

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Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments

Cited by 29 publications

References 45 publications

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Immune response to recombinant Burkholderia pseudomallei FliC

Presence of Burkholderia pseudomallei in the ‘Granary of Myanmar’

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