To rcducc connective tissue toughness V&i0 B-30 coflagenase was evaluated for its ability to degrade muscle collagen in a rcstructurcd beef (RSB) product. Dissected muscles pooled from clods of 4 USDA choice chucks were made into three RSB products: untrimmed control (C), hand trimmed of cpimysium (T) and untrimmed and treated with 0.01% collagenase (E). RSB products were held for 24 hr at either 2°C or 11°C and were analyzed either raw or after heating (40°C for 1 hr). Aerobic microbial load remained low (l-2X102 cfu/g) during the 24 hr 11°C incubation. Heated samples of enzyme treated RSB had lower Lee-Kramer shear values than controls. In both raw and heated samples enzyme treatment increased hydroxyproline solubility. An cnzymc treatment of 24 hr at 11°C followed by heating produced maximum tcnderization. Although optimum treatment conditions need to be dcfincd by further research, Vihrio B-30 collagenase appeared to be efficacious for the tendcrization of RSB products.