Effect of temperature on invasion of MO4 mouse fibrosarcoma cells in organ culture

Mareel, Marc; Bruyneel, Eric A.; Dragonetti, C.; Bruyne, Georges K. De; Cauwenberge, Rita M.-L. Van; Smets, L. A.; Rooy, Henny van

doi:10.1007/bf00052412

Cited by 15 publications

(3 citation statements)

References 23 publications

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“…These variations relate to the origin of the host tissue, the presentation of the confronting test cells, and the incubation conditions. Heart fragments from species other than chick 18 , and from tissues such as liver 19 , lung 20 , and brain 21 , have been examined. Instead of aggregates, biopsy specimens , and serum-free confrontations have been shown to be feasible with certain types of cells 25 .…”

Section: Discussionmentioning

confidence: 99%

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Bracke¹,

Roman

Stevens

et al. 2015

JoVE

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The goal of the chick heart assay is to offer a relevant organ culture method to study tumor invasion in three dimensions. The assay can distinguish between invasive and non-invasive cells, and enables study of the effects of test compounds on tumor invasion. Cancer cells -either as aggregates or single cells -are confronted with fragments of embryonic chick heart. After organ culture in suspension for a few days or weeks the confronting cultures are fixed and embedded in paraffin for histological analysis. The three-dimensional interaction between the cancer cells and the normal tissue is then reconstructed from serial sections stained with hematoxylin-eosin or after immunohistochemical staining for epitopes in the heart tissue or the confronting cancer cells. The assay is consistent with the recent concept that cancer invasion is the result of molecular interactions between the cancer cells and their neighbouring stromal host elements (myofibroblasts, endothelial cells, extracellular matrix components, etc.). Here, this stromal environment is offered to the cancer cells as a living tissue fragment. Supporting aspects to the relevance of the assay are multiple. Invasion in the assay is in accordance with the criteria of cancer invasion: progressive occupation and replacement in time and space of the host tissue, and invasiveness and non-invasiveness in vivo of the confronting cells generally correlates with the outcome of the assay. Furthermore, the invasion pattern of cells in vivo, as defined by pathologists, is reflected in the histological images in the assay. Quantitative structure-activity relation (QSAR) analysis of the results obtained with numerous potentially anti-invasive organic congener compounds allowed the study of structure-activity relations for flavonoids and chalcones, and known anti-metastatic drugs used in the clinic (e.g., microtubule inhibitors) inhibit invasion in the assay as well. However, the assay does not take into account immunological contributions to cancer invasion. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Bracke¹,

Roman

Stevens

et al. 2015

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“…Virally transformed C3H mouse fibroblastic cells [1], which are invasive and metastatic in syngeneic mice [23], are not invasive at temperatures of 29°C or cooler but invade at 30"5°C or higher when cocultured with embryonic chick heart for seven days [9]. Virally transformed C3H mouse fibroblastic cells [1], which are invasive and metastatic in syngeneic mice [23], are not invasive at temperatures of 29°C or cooler but invade at 30"5°C or higher when cocultured with embryonic chick heart for seven days [9].…”

Section: Discussionmentioning

confidence: 99%

Invasion in vitro by explants of Lucke renal carcinoma cocultured with normal tissue is temperature dependent

McKinnell

Bruyneel²,

Mareel³

et al. 1986

Clin Exp Metast

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Fragments of renal carcinoma of the northern leopard frog, Rana pipiens, were cocultured in vitro with small pieces of tadpole heart, frog heart and frog kidney with gyrotory shaking for up to 14 days at 21 degrees C and 28 degrees C. No invasion by renal carcinoma occurred in confrontation cultures at 21 degrees. However, the three normal tissues were invaded by renal carcinoma in confrontation cultures incubated at 28 degrees C. Invasion in vitro by histologically typical renal carcinoma is thus similar to temperature-dependent invasion by the renal carcinoma-derived cell line PNKT-4B and affords an opportunity for the identification of cell or biochemical events which may be activated at invasion-permissive temperature. Cell or biochemical events which are selectively activated and subsequently repressed as the renal tumor is incubated at invasion-permissive and invasion-restrictive temperatures become significant candidates as events involved in, or causal for, malignant invasion.

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“…It has been shown that invasion in vitro of mouse MO 4 cells [15], of frog Luck~ renal carcinoma explants [19] and of frog PNKT-4B carcinoma cells [20] can be inhibited by lowering the temperature of incubation. In short-term experiments invasion in vitro of MO 4 cells into chick heart showed a transition between 30"5 and 29~ invasion occurred after 4 days of incubation at 30"5~ but not after 7 days at 29~ [15]. As a possible explanation, we considered that absence of invasion was due to alterations in the expression of glycopeptides at the surface of MO 4 cells.…”

Section: Introductionmentioning

confidence: 99%

Restored invasion of mouse MO4 cells into chick heartin vitro through mutual conditioning at reduced temperature

et al. 1989

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Invasion of malignant mouse MO4 cells into embryonic chick heart fragments in confronting organ cultures was arrested for 7 days when the temperature of incubation was lowered to 28 degrees C. Afterwards invasion resumed and progression between days 10 and 17 at 28 degrees C was comparable to that between days 0 and 7 at 37 degrees C. This pattern of progression of MO4 cell invasion at 28 degrees C was unaltered when either MO4 cells or heart fragments or both were preincubated separately at 28 degrees C for 14 days before confrontation with each other. Invasion at 28 degrees C resumed only when MO4 cells and heart tissue had been in immediate contact for at least 7 days. Metabolic labelling with [3H]fucose showed a correlation in time between transient suppression of invasion and transient inhibition of incorporation of fucosylation-precursor molecules into glycoproteins by MO4 cells. The latter activity was far less temperature-sensitive in heart cells. Our observations suggest that metabolic cooperation between invading MO4 cells and heart tissue is essential for progression of invasion in vitro.

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Effect of temperature on invasion of MO4 mouse fibrosarcoma cells in organ culture

Cited by 15 publications

References 23 publications

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Invasion in vitro by explants of Lucke renal carcinoma cocultured with normal tissue is temperature dependent

Restored invasion of mouse MO4 cells into chick heartin vitro through mutual conditioning at reduced temperature

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