Effect of temperature on microbial diversity and nitrogen removal performance of an anammox reactor treating anaerobically pretreated municipal wastewater

Abstract

The effects of temperature reduction (from 35 °C to 20 °C) on nitrogen removal performance and microbial diversity of an anammox sequencing batch reactor were evaluated. The reactor was fed for 148 days with anaerobically pretreated municipal wastewater amended with nitrite. On average, removal efficiencies of ammonium and nitrite were high (96%) during the enrichment period and phases 1 (at 35 °C) and 2 (at 25 °C), and slightly decreased (to 90%) when the reactor was operated at 20 °C. Deep sequencing analysis revealed that microbial community structure changed with temperature decrease. Anammox bacteria (Ca. Brocadia and Ca. Anammoximicrobium) and denitrifiers (Burkholderiales, Myxococcales, Rhodocyclales, Xanthomonadales, and Pseudomonadales) were favoured when the temperature was lowered from 35 °C to 25 °C, while Anaerolineales and Clostridiales were negatively affected. The results support the feasibility of using the anammox process for mainstream nitrogen removal from anaerobically pretreated municipal wastewater at typical tropical temperatures.

Ditching Nutrients: Roadside Drainage Networks are Hotspots for Microbial Nitrogen Removal

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Convergent community structure of algal–bacterial consortia and its effects on advanced wastewater treatment and biomass production

“…4 . Proteobacteria 29 , Cyanobacteria 30 , 31 , Bacteroidetes 15 , Chloroflexi 32 , Nitrospirae 33 with these consortia were related to nutrient removal. However, Proteobacteria remained the dominant species with consortia, microbial function shown in Table 3 .…”

“…However, the ability of Proteobacteria to remove nitrogen and phosphorus was not significant because the dominant Species of nitrogen and phosphorus was Chlorella. Therefore, the ability of Proteobacteria, such as α- Proteobacteria, β-Proteobacteria, and γ-Proteobacteria, to remove organic matter was significant 29 . The CO 2 and low molecular weight organic matter produced by Proteobacteria oxidation are provided to microalgae community.…”

Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox‐based processes

“…Moreover, the temperature of municipal wastewater varies seasonally, which might have a negative effect on the system. It has been reported that AnAOB have their maximum nitrogen removal efficiency at the medium temperature about 35°C (de Almeida Fernandes et al., 2018). In practice, the temperature in most wastewaters is much lower (10–20°C) than the optimal value, and it may even be lower than 10°C in high‐latitude areas.…”