Effect of temperature on muscle lactate metabolic recovery in sea bass (Dicentrarchus labrax, L.) juveniles exposed to exhaustive exercise

Abstract

Muscle lactate metabolic recovery in sea bass juveniles was studied in respect to the water temperature. The fish used in this study were cultured with the semi-extensive mesocosm method, and the water temperatures were 15 and 20 degrees C. Fish were led to exhaustion by exercising in a swimming channel, and the muscle lactate levels were measured seven times before and after exercise. The results showed that muscle lactate concentration rises during exercise until it reaches a maximum (exhaustion point), and then it keeps falling during the recovery time. Also, fish exercised at 20 degrees C exhibited higher lactate values at the exhaustion point and faster decomposition rates during recovery.

Exercise–related muscle lactate metabolism in zebrafish juveniles: The effect of early life temperature

“…Fish were euthanized with ethyleneglycolmonophenylether (Merck, 0.2-0.5 ml l -1 ) and muscle tissue of the haemal part of the body was removed and stored at -80 • C for further analysis (McClelland et al 2006). The LactPap kit (61.192, bioMérieux, France) was used for lactate concentration measurement according to the method described by Barnett & Pankhurst (1998) and validated for zebrafish by Sfakianakis & Kentouri (2010). Data were afterwards analyzed by means of non-parametric Kruskal-Wallis and Mann-Whitney U tests, since the assumptions for normality and homoscedasticity were not met.…”

“…Temperature can affect time of hatching, growth, metabolism (Herzig & Winkler 1986), muscle ontogeny and development (Johnston 1993), swimming performance (Koumoundouros et al 2002a;Sfakianakis et al 2011a), external morphology and meristic characters (Turan 2004;Sfakianakis et al 2011b), osteological development and appearance of skeletal deformities (Koumoundouros et al 2001;Sfakianakis et al 2004), sex determination (Koumoundouros et al 2002b) and the overall survival of fish (Lein et al 1997). Exercise-associated fish metabolism is also among the characteristics that are significantly affected by temperature (Sfakianakis & Kentouri 2010).…”

“…Lactate dehydrogenase, which is the terminal enzyme of anaerobic glycolysis in vertebrates, is correlated to anaerobic potential and locomotory performance (Fiedler et al 1998). There are numerous studies regarding muscle lactate concentration and its removal rate in various farmed species (Young & Cech 1993Milligan 1996;Davison 1997;Milligan et al 2000;Richards et al 2002;Sfakianakis & Kentouri 2010) and some also acknowledge the effect of swimming temperature (Milligan et al 2000;Richards et al 2002;Sfakianakis & Kentouri 2010). However, up to now there haven't been any studies correlating early life temperature and lactate metabolism at later ontogenetic stages.…”

Chill out: physiological responses to winter ice-angling in two temperate freshwater fishes

“…The response of organisms to stress may also be influenced by environmental conditions, including temperature (Wieser et al, 1986; Lankford et al, 2003; Davis, 2004). In the case of fish, studies have indicated that high temperatures can lead to a more pronounced hormonal (Barton and Schreck, 1987; Jaxion-Harm and Ladich, 2014) or metabolic response (Kieffer et al, 1994; Sfakianakis and Kentouri, 2010). Cold temperatures, on the other hand, have often been found to dampen the magnitude of this response (Van Ham et al, 2003; Davis, 2004; Guderley, 2004).…”

Developmental toxicity assay using high content screening of zebrafish embryos

“…Although we do not report mortality in the current study, it is likely that deformed embryos would not survive. In fish, the appearance of morpho-anatomical deformities can be associated with temperature (Wang and Tsai, 2000;Koumoundouros et al, 2009;Sfakianakis and Kentouri, 2010). There is a lack of published data on the influence of temperature on fish body shape and especially on specimens reared under laboratory conditions.…”