Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns

Beltrán, Roberto; Valls, A.; Cebrián, Nuria; Zornoza, Carlos; García‐Breijo, Francisco J.; Armiñana, José Reig; Garmendia, Alfonso; Merlé, Hugo

doi:10.7717/peerj.8195

Cited by 16 publications

(12 citation statements)

References 39 publications

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“…This can affect fruit and seed setting ( Vasilakakis and Porlingis, 1985 ; Sukhvibul et al., 2000 ; Pirlak, 2002 ; Acar and Kakani, 2010 ). The optimal temperature for pollen germination of most plants ranges from 20°C to 28°C ( Sorkheh et al., 2018 ; Beltrán et al., 2019 ; Iovane et al., 2021 ), and only a few species require higher ( Mbogning et al., 2007 ; Cao et al., 2016 ; Fan et al., 2018 ) or lower ( Li et al., 2005 ) temperatures. Excessively high or low incubation temperature can reduce pollen germination.…”

Section: Discussionmentioning

confidence: 99%

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Jia

Wang²,

Mi³

et al. 2022

Front. Plant Sci.

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Pollen morphology, pollen vigor, and long-term pollen storage are critical for plant cross-breeding and genetic improvement of Exochorda racemosa. We developed a protocol for viability determination and storage of E. racemosa pollen for breeding new varieties. The medium components for E. racemosa pollen germination was optimized by using an Orthogonal Assay Test Strategy (OATS). The germination rates of E. racemosa pollen were investigated after storing at different temperatures and different storage periods. The size of E. racemosa pollen was medium with three germination ditches, and the sculptural type of pollen was striate. Red ink and 2,3,5-triphenyl tetrazolium chloride (TTC) can effectively distinguish viable pollen from the unviable pollen of E. racemosa. The most suitable medium (CK2) for E. racemosa was composed of 150 g· L–1 sucrose, 100 mg·L–1 boric acid, 150 mg· L–1 Ca(NO3)2 and 50 mg· L–1 GA3. Low-temperature stress produced the greater inhibition of pollen tube growth compared with high-temperature conditions. The CK2 medium at pH 6.5 resulted in the highest pollen germination rate and most extended pollen tube length. The optimal temperature for storage of dried pollen was –80°C (P < 0.01), and the germination rate was 53.60% after storage for 390 days. Thawing in a 35°C water bath produced the best viability of E. racemosa pollen after storage at –20°C and –80°C. The short-term storage of E. racemosa fresh pollen at 4°C was better than that at –20°C and –80°C (P < 0.01). It is possible to evaluate pollen quality and store pollen grains for E. racemosa by the parameters defined in this study.

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Section: Discussionmentioning

confidence: 99%

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Jia

Wang²,

Mi³

et al. 2022

Front. Plant Sci.

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“…Commonly reported incubation times for apple pollen range from 2 h [ 12 ] to 24 h [ 34 ] but can be as high as 32 [ 15 ] or 72 h [ 16 ]. The effect of incubation on pollen germination and pollen tube length follows a linear plateau model.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro germination assays have been used since the early 1900s to investigate a broad set of research questions regarding pollen viability and performance. Since then, several research groups have focused on optimizing certain conditions, such as incubation temperature [ 12 , 13 ], germination media [ 12 , 14 ], and storage conditions [ 15 , 16 ]. There are currently over 35 published germination media for apple pollen that contain different amounts of sucrose, boric acid, calcium nitrate, magnesium sulfate, and potassium nitrate.…”

Section: Introductionmentioning

confidence: 99%

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Roeder

Serra

Musacchi

2021

Plants

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In vitro germination assays are frequently used in screening trials to evaluate the pollen viability of pollinizers. To be effective, screening trials must have defined threshold criteria, from which individuals can then be assessed. However, despite decades of research on pollen viability, no established threshold is available to categorize apple cultivars based on their in vitro pollen tube lengths. This study aimed to identify and characterize the subgroups of cultivars based on their pollen tube growth performance. In vitro pollen tube lengths of 41 individuals were determined by incubating samples on artificial germination media at 15 and 25 °C. A six-number summary statistic was calculated, and hierarchical clustering on principal component (HCPC) analysis was used to determine and characterize subgroups. Furthermore, a decision tree model was used to predict class membership for future datasets. HCPC analysis partitioned the 41 individuals into three subgroups with different performances. The decision tree quickly predicted the cluster membership based on the second quartile at 15 °C and the third quartile at 25 °C. The thresholds from the decision tree can be used to characterize new observations. The use of the methods will be demonstrated using a case study with 29 apple accessions.

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“…Successful cryopreservation of Malus pollen has been reported many times. According to the published data, this pollen stored in liquid nitrogen can retain viability for at least 15 years [78][79][80].…”

Section: Cryobiotechnology Of Malus (Apple)mentioning

confidence: 99%

Cryobiotechnology of Plants: A Hot Topic Not Only for Gene Banks

Jiroutová

Sedlák

2020

Applied Sciences

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Agriculture has always been an important part of human evolution. Traditionally, farming is changing and developing with regard to challenges it faces. The major challenges of modern agriculture are food and nutrition safety for the growing world population. Promoting species and genetic diversity in agriculture appears to be an important approach to dealing with those challenges. Gene banks all around the world play a crucial role in preserving plant genetic resources for future crop improvements. The plant germplasm can be preserved in different ways, depending on the species or form of stored plant tissue. This review focuses on a special preservation method—cryopreservation. Cryopreservation is an effective technique for storing living systems at ultra-low temperatures, usually in liquid nitrogen or its vapor phase. This conservation method is crucial for plants that do not produce seeds or that produce non germinating seeds, as well as for plants that propagate vegetatively. Moreover, based on the cryopreservation method, a novel plant biotechnology tool for pathogen eradication called cryotherapy has been developed. The use of liquid nitrogen eliminates plant pathogens such as viruses, phytoplasmas, and bacteria. Our article reviews recent advances in cryo-biotechnologies such as cryopreservation and cryotherapy, with special focus on studies concerning fruit plants.

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Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns

Cited by 16 publications

References 39 publications

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Cryobiotechnology of Plants: A Hot Topic Not Only for Gene Banks

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