Effect of temperature on the separation of long DNA fragments in polymer solution

Multiplexed DNA sizing by capillary electrophoresis using entangled polymer solutions and diode array detection

“…As expected, the migration times of the DNA fragments also decreased as temperature increased, mainly because higher temperatures decreased the viscosity of the separation matrix [22][23][24][25][26][27][28]. Although most fragments have been well separated (except the fragments with similar size) the linearity of separation was poor.…”

Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy

“…21 Also, it used a method to control DNA conformation, 22 and the dependence of single-cut l-DNA fragment mixture mobility on various frequencies at three different temperatures showed that antiresonance mobility minima shifted to higher frequencies at higher temperatures. 23 Consequently, our results revealed a large difference in terms of direction and velocity between individual native-DNA and YOYO-DNA molecules by the application of an electric field, temperature, and the effect of EOF suppression polymers in microchips.…”

Identification and quantification of GC-rich oligodeoxynucleotides in tissue extracts by capillary gel electrophoresis

“…Various strategies have been tested in the past in an effort to improve the denaturing power of gel electrophoresis. Elevated temperatures were found helpful in CGE of long DNA fragments by reducing separation time and by resolving compressions [20,26,27] as well as by improving selectivity [28,29]. Furthermore, addition of different organic solvents was reported to improve peak resolution of the CGE.…”