Effect of temperature on the inhibition kinetics of phenol biodegradation byPseudomonas putida Q5

Onysko, Kira A.; Budman, Hector; Robinson, Campbell W.

doi:10.1002/1097-0290(20001105)70:3<291::aid-bit6>3.0.co;2-y

Cited by 80 publications

(50 citation statements)

References 38 publications

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“…This is within the accepted range of 1.01 to 1.07, with 1.04 being a typical value for biological activity (36). Although Onysko et al, (30) had reported a similar temperature dependency for both K i and K s for phenol, this was not evident for our nonylphenol studies. More experiments on temperature effects may have to be conducted to obtain the impact of temperature on K i and K s .…”

Section: Resultscontrasting

confidence: 57%

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Modeling Biodegradation of Nonylphenol

Jahan

Ordóñez

Ramachandran

et al. 2007

Water Air Soil Pollut: Focus

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Abstract. Nonylphenol is the primary breakdown product of nonylphenol ethoxylates (NPEs), a certain class of nonionic surfactants. Nonylphenol has been found to be toxic to aquatic organisms and has been suspected of being harmful to humans due to its xenoestrogenic properties. Although there are known releases of nonylphenol to the environment, there is a lack of data describing the extent of biodegradation. This study thus focuses on much needed information on the biodegradation kinetics of nonylphenol. Oxygen uptake, cell growth and nonylphenol removal data were collected using batch reactors in an electrolytic respirometer. Nonylphenol removal, cell growth and substrate removal rates were modeled by the Haldane, Aiba, Webb, and Yano equations. The differential equations were solved by numerical integration to simulate cell growth, substrate removal, and oxygen uptake as a function of time. All models provided similar results with the Haldane model providing the best fit. The values of the kinetic parameters and the activation energy for nonylphenol were determined. These values can be used for predicting fate and transport of nonylphenol in the environment. The validity of applying each model to the biodegradation of nonylphenol was analyzed by computing the R 2 values of each equation.

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Section: Resultscontrasting

confidence: 57%

“…Haldane ( Biodegradation rates are also impacted by temperature. The Arrhenius model has been used extensively in biological and engineering literature over limited temperature ranges (30) in quantifying the effect of temperature on reaction rates. The Arrhenius equation for cell growth rate µ m is presented below:…”

Section: Modeling Np Biodegradationmentioning

confidence: 99%

Modeling Biodegradation of Nonylphenol

Jahan

Ordóñez

Ramachandran

et al. 2007

Water Air Soil Pollut: Focus

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“…The specific growth and phenol degradation rates were calculated by performing a linear regression on initial curves of cell growth and phenol disappearance, respectively, against time. Biomass concentrations on a dry weight basis were determined by filtering the cell suspension through a 0.2-m-pore filter (cellulose acetate membrane filter; Advantec MFS, Inc. Dublin, Calif.) and drying the filter and cells to a constant weight for 24 h at 80°C (31). A linear relationship between the OD and dry weight was observed.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Diversity and Function of Aerobic Granules Engineered in a Sequencing Batch Reactor for Phenol Degradation

Jiang

Tay

Maszenan

et al. 2004

Appl Environ Microbiol

121

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Aerobic granules are self-immobilized aggregates of microorganisms and represent a relatively new form of cell immobilization developed for biological wastewater treatment. In this study, both culture-based and cultureindependent techniques were used to investigate the bacterial diversity and function in aerobic phenol-degrading granules cultivated in a sequencing batch reactor. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes demonstrated a major shift in the microbial community as the seed sludge developed into granules. Culture isolation and DGGE assays confirmed the dominance of ␤-Proteobacteria and high-G؉C gram-positive bacteria in the phenol-degrading aerobic granules. Of the 10 phenol-degrading bacterial strains isolated from the granules, strains PG-01, PG-02, and PG-08 possessed 16S rRNA gene sequences that matched the partial sequences of dominant bands in the DGGE fingerprint belonging to the aerobic granules. The numerical dominance of strain PG-01 was confirmed by isolation, DGGE, and in situ hybridization with a strain-specific probe, and key physiological traits possessed by PG-01 that allowed it to outcompete and dominate other microorganisms within the granules were then identified. This strain could be regarded as a functionally dominant strain and may have contributed significantly to phenol degradation in the granules. On the other hand, strain PG-08 had low specific growth rate and low phenol degradation ability but showed a high propensity to autoaggregate. By analyzing the roles played by these two isolates within the aerobic granules, a functional model of the microbial community within the aerobic granules was proposed. This model has important implications for rationalizing the engineering of ecological systems.

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“…• C (Onysko et al, 2000). Temperature is one of the critical factors in designing a biological treatment process and hence studying temperature effects on microbial kinetics.…”

Section: Effect Of Environmental Factorsmentioning

confidence: 99%

Aerobic degradation and metabolite identification of the N-heterocyclic indole by the Pseudomonas putida strain mpky-1 isolated from subtropical mangrove sediment

Yip¹,

Gu²

2017

Appl Env Biotechnol

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Aerobic biodegradation and metabolite identification of indole by the Pseudomonas putida strain mpky-1 isolated from coastal sediment of the Inner Deep Bay of Hong Kong was investigated in this study. This strain had 99.1% similarity with P. putida known. The biochemical degradation pathway of indole involved an initial hydroxylation reaction at the C-2 position to form oxindole followed by a second hydroxylation at the C-3 position to isatin prior to the cleavage of the 5-member carbon ring. This bacterium grew better at 22• C though it was capable of growth at low temperature (15• C in this study) with a longer lag phase. Both the bacterial specific growth rate and the biodegradation rate increased from 0.0035/hr to 0.0249/hr and from 15• C to 30• C, respectively. P. putida mpky-1 grew quicker at pH 6.4 (specific growth rate, 0.0115/hr) than pH 7.4 (specific growth rate, 0.0066/hr) and pH 8.4 (specific growth rate, 0.036/hr) although the lag time of bacterial growth at pH 7.4 and pH 8.4 (15.01 hr and 15.00 hr, respectively) was very similar. The decrease in bacterial growth rate was observed when salinity increased from 5‰ to 30‰. P. putida mpky-1 may adapt to the Mai Po and Inner Deep Bay and degrade indole due to the polluted condition. Keywords: aerobic degradation, indole, heterocyclic aromatics, metabolic pathway, Pseudomonas putida, environmental factors * Correspondence to: Ji-Dong Gu, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Email: jdgu@hku.hk

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Effect of temperature on the inhibition kinetics of phenol biodegradation byPseudomonas putida Q5

Cited by 80 publications

References 38 publications

Modeling Biodegradation of Nonylphenol

Modeling Biodegradation of Nonylphenol

Bacterial Diversity and Function of Aerobic Granules Engineered in a Sequencing Batch Reactor for Phenol Degradation

Aerobic degradation and metabolite identification of the N-heterocyclic indole by the Pseudomonas putida strain mpky-1 isolated from subtropical mangrove sediment

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