Effect of temperature on the photoproperties of luminescent terbium sensors for homogeneous bioassays

Abstract

We developed a luminescent terbium sensor (LTS) based on energy resonance transfer for homogeneous bioassays. The effect of temperature on photoluminescence and time-resolved fluorescence of the LTS was investigated. When the temperature was increased from 277 K to 369 K, the photoluminescence quantum yield decreased by up to 25 %, time-resolved fluorescence decreased by up to 54 %, and the lifetime shortened dramatically. Studies showed that both photoluminescence and time-resolved fluorescence quantum yields were largely recovered after samples were heated from 298 to 310, 333 or 369 K and subsequently cooled to 298 K. These results indicate that the homogeneous bioassay with LTS is sensitive to temperature and should be conducted at a constant temperature to ensure the temperature effect does not influence data and to increase the accuracy of the results. The results of this study are important for LTS applications in homogeneous bioassays.

Facilely Fabricated Luminescent Nanoparticle Thermosensor for Real-Time Microthermography in Living Animals

“…Microthermography in living animals, visualizing the temperature distribution in cells, tissues, and organisms with high spatial resolution on the order of 100 μm or smaller, allows for locating thermogenesis sites. To visualize temperature shift in such biological samples, varieties of temperature sensitive luminescent materials (probes) including organic molecular dyes, − quantum dots, − proteins, − and dye-embedded polymers, − whose luminescence intensity, lifetime, or spectrum-shift varies due to temperature shift, have been designed and synthesized . To date, most of the probes have been tested to demonstrate luminescence thermometry in cultured cells, − ,,,− ,, but just a few were examined in tiny living organisms, C.…”