Effect of temperature onin vitro pollen germination in pigeonpea

Singh, Inderjit; Bharti, S.; Nandwal, A. S.; Goswami, C. L.; Varma, S. K.

doi:10.1007/bf02923602

Cited by 7 publications

(6 citation statements)

References 4 publications

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“…The process of selecting pollen with improved heat tolerance could be accelerated with a rapid and reliable method of germinating cotton pollen to measure viability across a range of environmental stresses. Current pollen germination techniques include hanging drop culture , sitting drop suspension culture , suspension culture , and surface culture (Alexander and Ganeshan, 1989; Brewbaker and Kwack, 1963; Cheng and Freeling, 1976; Dawkins and Owens, 1993; Egea et al, 1992; Feijo et al, 1995; Frascaroli and Tuberosa, 1993; Mulcahy and Mulcahy, 1988; Pareddy and Petolino, 1992; Pundir, 1972; Shivanna and Rangaswamy, 1992; Shivanna et al, 1991; Singh et al, 1992; Tanaka et al, 1995; Tupy et al, 1983; Vogt et al, 1994; Yistra et al, 1995; Zonia and Tupy, 1995). The hanging drop and sitting drop cultures use only small volumes of germination media and small amounts of pollen and are therefore of limited usefulness in physiological and biochemical studies.…”

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confidence: 99%

In Vitro Analysis of Cotton Pollen Germination

Burke¹,

Velten²,

Oliver³

2004

Agronomy Journal

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Crops with economically valuable reproductive structures show the greatest negative discrepancy between average and record yields. To alleviate environmental stress–related yield reductions, a better understanding of the relative sensitivities of pollen development, dehiscence, pollen germination, pollen tube growth, fertilization, and subsequent boll development is needed. Progress in identifying the sensitivities of these developmental stress responses has been hampered in part by the lack of a rapid and reliable method of germinating cotton (Gossypium spp.) pollen in vitro. The present study describes the development of an in vitro cotton pollen germination system that provides improved pollen germination levels and pollen tube growth over existing methods. This procedure was used to evaluate the temperature sensitivity of pollen from greenhouse and field‐grown cotton. The medium comprises 10% (w/v) agarose (pH 7.6), 25% (w/v) sucrose, 0.52 mM KNO3, 3.06 mM MnSO4, 1.66 mM H3BO3, 0.42 mM MgS04·7H20, and 1.0 μM A3 gibberellic acid. The medium is overlayed with a layer of 1.5% agarose before use. Optimum pollen germination and rapid tube elongation occurred between 28 and 31°C under 80% relative humidity. Decreased pollen germination occurred at temperatures above 37°C, and decreased tube elongation occurred at temperatures above 32°C. Evaluation of pollen, taken at 1400 h from field‐grown cotton plants, showed that pollen from flowers exposed to direct sunlight had reduced viability compared with pollen from flowers inside the canopy. This could be attributed to the fact that flowers from field‐grown irrigated cotton plants exposed to full sunlight experienced internal temperatures as high as 39°C, well above the 28 to 31°C optimum for pollen germination.

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confidence: 99%

In Vitro Analysis of Cotton Pollen Germination

Burke¹,

Velten²,

Oliver³

2004

Agronomy Journal

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“…H-77-216 was 22°C while it was 27°C for cv. ICPL-151 21 . On the contrary, the highest rate of in vitro pollen germination (65%) was found at 20°C in eight genotypes of apricot 15 .…”

Section: Resultsmentioning

confidence: 99%

Morphology, viability, and germinability of pollen from two forms of Nymphaea nouchali var. versicolor, a day-blooming waterlily

Bodhipadma¹,

Noichinda²,

Thaiyanto³

et al. 2013

ScienceAsia

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Pollen morphology, viability, and germinability of two distinct forms (Bua Phan and Bua Phuean) of Nymphaea nouchali var. versicolor, one of the only two naturally occurring native varieties of the day-blooming waterlily in Thailand, were studied. Under a scanning electron microscope, the pollen morphology of the two forms of N. nouchali was very similar. The pollen grains were nearly ellipsoidal, heteropolar, round-shaped from one side and boat-shaped from the other side, and had a smooth surface and a ring-like aperture around the equator. There was no significant difference between the pollen viability (95%) of the Bua Phan and Bua Phuean forms examined after acetocarmine staining. Pollen germinability was evaluated on a medium supplemented with various concentrations of sucrose under different incubation temperatures and illumination conditions. The highest percentages of pollen germination in the Bua Phan (93%) and Bua Phuean (80%) forms took place at 32°C in the dark on a medium supplemented with 5% sucrose. These germination results were significantly different. The dissimilar germination response is also consistent with the current classification of the versicolor variety into two forms.

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“…Studies on temperature stress, generally are often heat shock treatments with temperature spikes applied in short bursts, from a few hours to a few days during the life span (Elgersma et al, 1989;Fang et al, 2010;Hedhly et al, 2004;Singh et al, 1992) and cannot be directly compared to our results of altered growing environments. But, male functions have been shown to be more susceptible to temperature variations and instrumental in largescale drop in plant productivity (Hedhly, 2011;Hedhly et al, 2004Hedhly et al, , 2009.…”

Section: Discussionmentioning

confidence: 99%

Drought and temperature stresses impact pollen production and autonomous selfing in a California wildflower, Collinsia heterophylla

Arathi

2023

Ecology and Evolution

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Ongoing climatic changes have altered growing conditions for plants by limiting water availability and inducing unprecedented temperature increases, eliciting plant functional responses that compromise floral trait expression, reduce pollen production and promote early self-pollination. In a controlled greenhouse study, plants of Collinsia heterophylla, an annual mixed-mated hermaphrodite, were grown under temperature and water stresses. Floral trait responses and related effects on plant reproductive success were recorded. Plants grown under temperature and water stresses were shorter and had fewer leaves at flowering than control plants. Temperature-stressed plants flowered earlier and had smaller flowers that produced fewer per capita pollen grains than control and water-stressed plants. While lifetime flower production in plants experiencing temperature stress alone or water stress alone was similar to control plants, those receiving combined temperature and water stresses had significantly lower lifetime flower production. Abiotic stress did not affect investment in female traits such as ovule number per flower, but impacted male traits seen as fewer pollen grains per flower. In plants experiencing abiotic stress, an increase in autonomous self-pollination was facilitated through a compromise in herkogamy, a sexual interference mechanism that promotes cross-pollination. Spatial separation between stigma and anther, a measure of herkogamy was zero or negative in stressed plants. Thus, abiotic stress responses in plants did not compromise plant reproductive success as autonomous selfing was augmented. In nature, such responses will likely reduce available nutrition for pollinators through decreased flower and pollen production.

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Effect of temperature onin vitro pollen germination in pigeonpea

Cited by 7 publications

References 4 publications

In Vitro Analysis of Cotton Pollen Germination

In Vitro Analysis of Cotton Pollen Germination

Morphology, viability, and germinability of pollen from two forms of Nymphaea nouchali var. versicolor, a day-blooming waterlily

Drought and temperature stresses impact pollen production and autonomous selfing in a California wildflower, Collinsia heterophylla

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