Effect of temperature reduction on the reactivity of the mouse vas deferens to adrenergic drugs

Souilem, Ouajdi; Bidon, J C; Gogny, Marc; Blin, Maryline; Jondet, A

doi:10.1016/0742-8413(92)90181-6

Cited by 6 publications

(11 citation statements)

References 11 publications

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“…Isolated tissues were prepared as previously described (Souilem, Bidon, Gogny, Blin & Jondet, 1992). Briefly, mice fasted overnight were killed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

“…Contractile responses were recorded as previously described by Souilem et al (1992). Briefly, vas deferens strips were attached at one end to a force isometric transducer using inductive proximity sensor (Electro-mike from Electrocorporation, Sarasota, FL, USA) and submitted to a basal resting tension (see above).…”

Section: Isometric Measurements Of Smooth Muscle Reactivitymentioning

confidence: 99%

“…Total CKC were statistically evaluated using the twoway analysis of variance test (ANOVA) with repeated measures, and all pairs of points were then compared by the contrast method. For comparing specific curve parameters (pD2 defined as -log ECso and maximal contraction as En,), the CRC of the preparations were analysed by means of a computerized curvefitting technique based on the four parameter logistic equation (De Lean, Munson & Rodbard, 1978;Meddings, Scott & Fick, 1989;Souilem et al, 1992). Levels of significance between the parameters were calculated using either the paired or unpaired Student's t-test for dependent or independent samples, respectively.…”

Section: Stutisticsmentioning

confidence: 99%

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Effect of temperature reduction on the vas deferens hyperresponsiveness of sensitized mice

Bidon

Souilem

Gogny

et al. 1995

Journal of Autonomic Pharmacology

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1. The present study was designed to investigate the effect of active sensitization on the responsiveness of mouse vas deferens before and after moderate cooling. Contractile responses to noradrenaline (NA) were isometrically recorded in the vas deferens of control and ovalbumen-sensitized mice at 37 degrees C and 22 degrees C. 2. Enhancement of the vas deferens reactivity to NA was observed in the sensitized vs control mice at 37 degrees C and 22 degrees C (P < 0.01). In sensitized mice, maximal contraction was significantly increased compared with controls, and sensitization-induced hyperresponsiveness was greater at 22 degrees C compared with 37 degrees C. At 37 degrees C, contractile responses to barium chloride were significantly enhanced in the sensitized mice compared with controls, whereas the reduction of the temperature to 22 degrees C produced a marked inhibition of vas deferens contractions in both groups. Caffeine-induced contractions of the vas deferens were similar in control and sensitized mice at 37 degrees C. After moderate cooling to 22 degrees C, vas deferens from sensitized mice became hyperresponsive compared with controls. 3. Ouabain (0.1 mM) produced an increase of NA-induced contraction in control and sensitized vas deferens at 37 degrees C (P < 0.01). It had no significant effect in the control at 22 degrees C but produced a marked inhibition of NA-induced contraction in the sensitized vas deferens at 22 degrees C. Contractions to NA in the presence of vanadate (1 mM) were depressed in control and sensitized mice at both temperatures. 4. These results suggest that sensitization-induced hyperresponsiveness of the mouse vas deferens is mediated by an increased mobilization of intracellular calcium. The involvement of an unknown ouabain-sensitive pathway in sensitization-induced alterations is also discussed.

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“…Isolated tissues were prepared as previously described (Souilem, Bidon, Gogny, Blin & Jondet, 1992). Briefly, mice fasted overnight were killed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

Section: Isometric Measurements Of Smooth Muscle Reactivitymentioning

confidence: 99%

Section: Stutisticsmentioning

confidence: 99%

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Effect of temperature reduction on the vas deferens hyperresponsiveness of sensitized mice

Bidon

Souilem

Gogny

et al. 1995

Journal of Autonomic Pharmacology

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“…The contractile response o f the preparation was recorded as previ ously desribed by Souilem et al [13]. Briefly, one side o f the ring was attached to an isometric force transducer using an inductive proximity sensor (Electro-mike from Elcctrocorporation, Sarasota, Fla., USA) and the other side fixed in the bath.…”

Section: Tracheal Reactivitymentioning

confidence: 99%

“…For comparing specific curve parameters (slope factor = S; pD2 = -log EC50, amplitude o f the maximal contraction = Em), the CRCs of the tracheal rings were ana lyzed by means o f a computerized curve-fitting technique based on the four-parameter logistic equation [13,15,16]. Levels of significance be tween the parameters obtained from control and sensitized animals were calculated using the unpaired Student's t test.…”

Section: Data Analysis and Statisticsmentioning

confidence: 99%

Strain-Related Differences in the Tracheal Responsiveness of Sensitized Guinea Pig

Bidon¹,

Blin²,

Gogny³

et al. 1995

Int Arch Allergy Immunol

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The purpose of this study was to evaluate the strain-related differences in tracheal hyperresponsiveness in control and egg albumen-sensitized guinea pigs. Concentration-response curves to acetylcholine and barium chloride were established from tracheal rings of Dunkin-Hartley and BEA strain guinea pigs. In the Dunkin-Hartley strain, sensitization did not signficantly increase the tracheal responsiveness to acetylcholine and barium chloride. By contrast, in the BFA strain, significant sensitization-induced hyperreactivity was achieved as the maximal contractions induced by acetylcholine and barium chloride, were enhanced from 6.5 ± 1.2 and 3.2 ± 0.4 mN in control to 10.0 ± 1.4 and 5.6 ± 0.8 mN, respectively, in sensitized animals. However, antigen challenge, performed in vitro, exhibited a similar amplitude of contraction in tracheal rings from both strains (Dunkin-Hartley 5.1+0.8 mN; BFA 5.9 ± 0.5 mN). Finally, while the two guinea-pig strains developed specific sensitization to allergen, only tracheal rings from the BFA strain developed hyperresponsiveness to acetylcholine and barium chloride. The strain-related difference appears to be partly explained by a lower basal reactivity in the BFA strain both to acetylcholine (E_m 7.3 ± 1.7 and 6.5 ± 1.2 mN for Dunkin-Hartley and BFA, respectively) and barium chloride (E_m 9.4 ± 2.6 and 3.2 ± 0.4 mN for Dunkin-Hartley and BFA, respectively). As the same procedure of sensitization provides different results in the genesis of hyperreactivity between the two guinea-pig strains used for asthma models, the BFA guinea-pig strain seems to be a better model because sensitized non-challenged animals could easily be dissociated from control ones, similar to that which occurs in asthmatic patients during provocation tests with cholinergic drugs.

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Effect of moderate cooling on contractile responses in mouse vas deferens and its relation to calcium

Souilem

Bidon

Gogny

et al. 1995

Naunyn-Schmiedeberg's Arch Pharmacol

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Isolated mouse vas deferens preparations were used to study the effect of temperature on noradrenaline-induced contractions. Preparations were suspended in the organ bath containing Krebs-Henseleit solution for isometric tension recording. Contractile responses to noradrenaline were investigated in the mouse vas deferens after moderate cooling from 37 to 26 or 22 degrees C. A significant increase of the phasic contractions to noradrenaline was observed at 26 or 22 degrees C compared with responses obtained at 37 degrees C (about 12.3 and 35.6% increase at 26 and 22 degrees C, respectively). The secondary noradrenaline-induced sustained contraction was also significantly enhanced after moderate cooling to 26 degrees C. The potentiation of noradrenaline-induced contraction at 26 degrees C remained in a Ca(2+)-free EGTA (1 mM)-containing solution. However, sustained contraction was suppressed after removal of the calcium from the medium at 37 and 26 degrees C. Contraction to caffeine was significantly enhanced at 22 degrees C compared with 37 degrees C. By contrast, barium chloride-induced contraction of the vas deferens was markedly decreased after moderate cooling to 22 degrees C. In the presence of ouabain (0.1 mM), the noradrenaline-induced peak contraction was significantly increased at 37 degrees C. However, potentiation of the noradrenaline response at 22 degrees C was unaffected by the Na+/K+ pump inhibitor. Noradrenaline-induced peak contractions were depressed in the presence of vanadate (1 mM) and cyclopiazonic acid (10 microM), two Ca(2+)-ATPase inhibitors, at 37 degrees C and also at 22 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effect of temperature reduction on the reactivity of the mouse vas deferens to adrenergic drugs

Cited by 6 publications

References 11 publications

Effect of temperature reduction on the vas deferens hyperresponsiveness of sensitized mice

Effect of temperature reduction on the vas deferens hyperresponsiveness of sensitized mice

Strain-Related Differences in the Tracheal Responsiveness of Sensitized Guinea Pig

Effect of moderate cooling on contractile responses in mouse vas deferens and its relation to calcium

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