Effect of temperature-sensitive nanogel combined with angioplasty on sICAM-1 and VE-cadherin in lower extremity arterial occlusion rabbits

Abstract

The study was to explore the effect of subintimal angioplasty (SIA) on the levels of soluble intercellular adhesion molecule-1 (sICAM-1) and vascular endothelial cadherin (VE-cadherin) in the rabbit model of lower extremity arterial occlusion. Specifically, the poly(N-isopropylacrylamide-co-butyl methacrylate) (PIB) temperature-sensitive nanogel was prepared, and the cytotoxicity of direct and indirect contact with PIB temperature-sensitive gel was analyzed then. Subsequently, the PIB temperature-sensitive gel was injected to the New Zealand white rabbit to prepare the lower extremity arterial occlusion model. The healthy control, model group, and SIA group were compared for the serum lipids, fibrinogen (Fbg), fibrinogen (Fbg), and fibrinogen (Fbg) levels. The results showed that the cell proliferation activity and survival rate were always higher than 90% under different concentrations of PIB temperature-sensitive gels. Compared with the model group, the SIA group had increased total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and Fbg levels, but decreased high-density lipoprotein (HDL) level ( P < 0.05); decreased TXB2, ET-1, and ICAM-1 levels, but increased levels of 6-Keto-PGF1α and NO ( P < 0.05); and decreased sICAM-1 and VE-cadherin levels ( P < 0.05). It showed that PIB temperature-sensitive nanogel can elicit vascular embolism, and SIA is suggested in the treatment of lower extremity arterial occlusion.

Effect of hypoxia and KCl depolarization in autofluorescence and ROS changes at the hippocampal CA3 area

“…Atherosclerotic occlusion is involved in endothelial cell dysfunction and migration, thrombus formation, and vascular remodeling with resultant local inflammation and cytokine release (Qi et al 2021). The high-fat diet-induced LEASO rats showed high levels of TC, IL-1, and TNF-α, Excessive apoptosis of endothelial cells is preliminary in atherosclerosis, and inhibiting apoptosis may prevent and treat atherosclerosis (Duan et al 2021).…”

Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

“…50 Previous studies have fully confirmed the strong biocompatibility of pNIPAm and QAS-pNIPAm. 51,52 γ-PGA, a common food and skin care additive, also exhibits excellent biocompatibility and degradability. 53,54 Another possible source for cytotoxicity of the nanogels is the release of CaP; however, in previous studies, Ca-induced cytotoxicity was specifically selective in tumour cells but biologically safe in normal cells.…”