Effect of tempol on post-thaw semen parameters and fertility during chicken semen cryopreservation

Abstract

The present study evaluated addition of tempol during chicken semen cryopreservation on post-thaw semen parameters and fertility. Adult PD-1 line semen was cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). In the semen cryomixture tempol (1 and 5 mM) was added at final concentrations. The semen with additives were filled in 0.5 ml French straws and exposed to liquid nitrogen vapours for 30 min and then stored in liquid nitrogen. The semen straw was thawed at 5 ° C for 100 sec and evaluated for sperm motility, live, abnormal and acrosome intact sperm. The seminal plasma was evaluated for lipid peroxidation. Fertilizing potential of the cryopreserved sperm was evaluated after insemination in the PD-1 line hens. The post-thaw sperm parameters were significantly (P<0.05) lower in the cryopreserved groups. The lipid peroxidation was significantly (P<0.05) higher in cryopreserved groups. The fertility was significantly (P<0.05) lower in all the cryopreserved groups. In conclusion, addition of tempol to cryopreservation mixture did not improve the post-thaw semen parameters or fertility.

Freezing ability of two slow permeating cryoprotectants on rooster semen diluted with tris egg-yolk juice extender

“…Choice of cryoprotectants seems to be highly significant in the cryopreservation process, considering their permeability, toxicity, including their application and suitability for different species of farm animals that vary. Furthermore, recent studies have investigated the use of slow permeating cryoprotectants such as dimethylformamide (DMF) and methylacetamide (MA) for rooster sperm cryopreservation with promising results (Sasaki et al 2010;Hanzawa et al 2006;Blanco et al 2012;Ehling et al 2012;Shanmugam and Mahapatra 2017). Based on several earlier reports of little to no contraceptive indications to hen's reproductive tract and comparatively low toxicity of MA and DMF cryoprotectants, this experiment therefore aimed at a testing the efficacy of these two slow permeating cryoprotectants and their combinations with tris quail egg-yolk orange and tomato juice supplemented extenders as cryoprotectants and extender of choice for roosters semen cryopreservation.…”