Effect of Testosterone on Human Chorionic Gonadotrophin (HCG)-Induced Ovarian Augmentation in Parabiotic Rats

Schuetz, Allen W.; Sager, Dorothea B.; Meyer, Roland K.

doi:10.1210/endo-75-3-383

Cited by 10 publications

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“…The ovaries were still more than double single control size, however, and follicular development was extensive, al¬ though the interstitial tissue was atrophie. This supports the view of Scheutz et al (1964) as well as Yasuda 8c Johnson (1965) that androgen inhibits the output of LH but not of FSH.…”

Section: Discussionsupporting

confidence: 83%

“…The present findings demonstrate that males have considerable FSH in their blood when 30 to 40 days of age. However, the ovaries of females in Groups XII and XIII are only about one third the size of those in partners of castra¬ tes (Scheutz et al 1964). While considered an indication of FSH, this type of test does not take into account the possible action of HCG on the release of gonadotrophin from the hypophysis of the male.…”

Section: Discussionmentioning

confidence: 99%

“…This technique has been used to detect endo¬ genous gonadotrophin in androgenized females (Johnson Sc Witscht 1963 b) and in castrate females (Scheutz et al 1964) to mention only a few applications. The present findings demonstrate that males have considerable FSH in their blood when 30 to 40 days of age.…”

Section: Discussionmentioning

confidence: 99%

“…Greep 8c Chester Jones (1950) failed in their attempt to analyze the effects of androgen on the gonadotrophins in female-hypophysectomized female pairs. Recently Scheutz et al (1964) included this com¬ bination in their experiments, but considered the gonadotrophins of the intact female too low to be detected. Johnson 8c Witscht (1963 ) observed increased ovarian weights in androgenized females which were joined to intact male rats.…”

mentioning

confidence: 99%

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The Use of Non-Castrate Parabiotic Rats for the Evaluation of Plasma Gonadotrophins

Johnson¹

1966

Acta Endocrinologica

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The gonads and accessory sex organs of hypophysectomized male or female rats were used to evaluate the gonadotrophin content of the plasma from a non-castrate parabiotic partner. Ovarian follicular development and the human chorionic gonadotrophin augmentation reaction indicated the presence of FSH in the plasma of immature males. LH was apparent by androgen production from testes in hypophysectomized male partners.The amount of LH was reduced by 2 mg progesterone or 20 \ g=m\ g testosterone propionate (TP) and increased by administration of hypothalamic extract daily to the intact male. Bilateral cryptorchidism also quickly elevated the plasma LH level. FSH output, as measured by follicular development in females, was not significantly affected by 50 \g=m\g TP or 2 \g=m\g oestradiol daily for 10 days. Females had little gonadotrophin in their plasma since hypophysectomized female partners showed only slight ovarian and no uterine stimulation. The increased gonadotrophins associated with unilateral compensatory hypertrophy, however, was quickly manifested in follicular hypertrophy and abundant oestrogen production in the hypophysectomized partner.The difficulties involved in the assay of hypophyseal gonadotrophins in plasma are generally recognized as a major impediment to a more complete under¬ standing of pituitary physiology. While luteinizing hormone (LH) (Parlow 1964) and follicle stimulating hormone (FSH) (Gans 1959) can be assayed in

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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The Use of Non-Castrate Parabiotic Rats for the Evaluation of Plasma Gonadotrophins

Johnson¹

1966

Acta Endocrinologica

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“…Schuetz, Sager & Meyer (1964) demonstrated that secretion of LH, but not of FSH, could be readily decreased by administration of exogenous steroids to the gonadectomized parabiotic partner. Because gonadectomy is not associated with increases in prolactin release (Neill, 1972) this hormone would not be expected to rise in sera of gonadectomized partners but it could be increased in their female partners as a consequence of increased oestrogen levels.…”

Section: Partnersmentioning

confidence: 99%

Changes in Follicle-Stimulating Hormone, Luteinizing Hormone, Prolactin, Oestradiol and Progesterone Concentrations in the Sera of Anovulatory Androgenized Female and Castrated Male Parabiotic Rats of the Fischer-344 Strain

Johnson¹

1981

Journal of Endocrinology

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The serum concentrations of FSH, LH and prolactin, as well as oestradiol and progesterone, were measured by immunoassays at various intervals up to 35 weeks after parabiotic union of an anovulatory androgenized female and a castrated male rat of the Fischer-344 strain. Modest increases in FSH (28%) and larger increases in LH (128%), compared to levels found in single adult animals, produced large increases in follicular growth, ovarian weight and oestrogen production in the female partners; thecal and interstitial elements of the ovaries were not heavily stimulated. Prolactin concentrations in the sera increased to exceedingly high levels and massive pituitary tumours were produced in all of the female partners. Development of cystic follicles with loss of granulosa-cell layers and the formation of early stages of granulosa-cell tumours progressed with time in union. Serum LH levels were as high in parabiotic as in single castrated male rats within 2 weeks, but concentrations of FSH remained much below those of the single animals until 27 weeks after castration. The results are consistent with the view that the ovaries of androgenized female rats of the Fischer strain produce some factor which prevents the post-castration rise in FSH of parabiotic male partners.

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Analysis of histophysiologic responses of the rat hypophysis to androgen treatment

Bogdanove

1967

Anat. Rec.

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The concentrations of LH and FSH were measured in the blood and adenohypophyses of castrated male rats exposed to high levels of testosterone propionate for 1 to 18 weeks. In intact male and female rats, similarly treated for 1 to 5 weeks, blood levels could not be measured but pituitary hormone levels were. Histological study of the pituitary gonadotrophic cells of all groups revealed a striking correlation between their regression, in response to androgen treatment, and the decline in pituitary LH stores. No light-microscopic correlate of the marked, progressive increase in pituitary FSH concentration was detected. Thus, when the pituitary FSH:LH ratio had increased 90 fold, all gonadotrophs appeared inactive.Although these studies failed to reveal the specific cellular source(s) of FSH and LH, they did clarify several aspects of the dynamics of the pituitary secretory responses to orchidectomy and androgen treatment. In the light of this information, the suggestion is made that the greatest dichotomy between pituitary FSH and LH stores is not coincident with -and probably occurs later than -that between LH and FSH secretion. Such asynchrony between the various phases of the FSH and LH secretory responses to these treatments (castration and/or androgen administration), may explain several seeming discrepancies among earlier reports on this subject. This paper is offered to the memory of William C. Young, with the hope that it may meet the criteria implicit in his charge (Young, '61) tomy itself increases both FSH and LH stores. Although they did not study FTH release directly, these authors suggested that pituitary hormone stores present a negative picture of release (i.e., -that testosterone blocks FSH release but promotes LH release, and that LH release is diminished in the absence of gonadal steroids). More direct studies have shown that much of this was incorrect. Orchidectomy does not increase pituitary FSH concentration significantly (Bogdanove et al., '64), although ovariectomy does (Parlow, '64). The reEease of both folliculotrophins is greatly increased by castration, in either sex (McCann and Ramirez, '64; Bogdanove, '64a, b; Parlow, '64).A similar attempt to interpret the effects of androgens on FTH release, by measuring pituitary hormone stores, was made by Beyler and Potts ('62). Using male rats, they found that TF' treatment prevented estrogen-induced depletion of pituitary FTH stores (which they believed to be mainly FSH ) . Assuming these sex steroids ' xxrork_ pceE$"_ted here was supported by grant -1. B-3371, U.S.P.H.S.2 Definitions of these components of FTH and of the phases of their seyetion have been presented elsewhere (Bogdanove, 64b; Bogdanove et al., '64).

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Effect of Testosterone on Human Chorionic Gonadotrophin (HCG)-Induced Ovarian Augmentation in Parabiotic Rats

Cited by 10 publications

References 0 publications

The Use of Non-Castrate Parabiotic Rats for the Evaluation of Plasma Gonadotrophins

The Use of Non-Castrate Parabiotic Rats for the Evaluation of Plasma Gonadotrophins

Changes in Follicle-Stimulating Hormone, Luteinizing Hormone, Prolactin, Oestradiol and Progesterone Concentrations in the Sera of Anovulatory Androgenized Female and Castrated Male Parabiotic Rats of the Fischer-344 Strain

Analysis of histophysiologic responses of the rat hypophysis to androgen treatment

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