Effect of Thawing Procedures on Fertility of Bovine Spermatozoa Frozen in .25-ml Straws

Rugg, C. D.; Berndtson, W. E.; Mortimer, Robert G.; Pickett, B.W.

doi:10.2527/jas1977.442266x

Cited by 7 publications

(2 citation statements)

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“…Forde and Gravir detected a better sperm motility at high temperatures such as 60 and 80°C. Rugg et al (1977) obtained very high pregnancy rates of 56.0 and 55.3% in thawing techniques of 12 sec at 35°C and 7 sec at 75°C, respectively. Foote (1998) stated that thawing process must be carried out fast to minimize the damage caused by the changes in ice crystals during the unfreezing of the sperm, so that, the motility, acrosomal integrity and potential fertility are maintained in the best possible way.…”

Section: Resultsmentioning

confidence: 88%

“…It is suggested that the sperm should be thawed for 30-40 sec at body temperature during field studies Nur et al, 2003;Rugg et al, 1977). Unless a specific recommendation is given, thawing for 30-40 sec in a 33-35°C water bath is recommended, regardless of the diluent type and freezing procedure (DeJarnette et al, 2000;DeJarnette and Marshall, 2005).…”

Section: Resultsmentioning

confidence: 99%

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Effect of Different Thawing Time and High Temperature on Frozen Thawed Bull Semen Traits

Yılmaz¹,

Ak²,

Baran³

2020

J. Anim. Vet. Adv.

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In this study was to investigate the effect of high temperature thawing and post-thaw cold shock application on sperm motility as well as acrosomal and total abnormalities of frozen bull semen. Four Holstein bulls were used to frozen semen in 0.25 mL straws. Semen of each bulls were thawed in 45 sec at 37°C (Group A = Control group) in 15 sec at 50°C (Group B) and 5 sec at 70°C (Group C) and post-thawing cold shock (300 sec at 5°C) were applied to all groups. After spermatozoa motility and morphological examinations are performed sperm samples were incubated at 35°C for 120 min and spermatological traits were repeated. Semen samples from the control and treatment groups were placed in an incubator taking into consideration the medium conditions (Modified buffered Hepes medium) and the time needed for spermatozoa to reach the fertilization site. Motility and morphological defects rates were determined with phase contrast microscopy and motility rate and speed with sperm fertility analyser (SFA-500). The Hancock's solution was used for morphologic examination of spermatozoa (acrosome, other and total). In computer aided sperm fertility analyser, motility and movement were performed in according to the technique. In conclusion, it has been found that the conventional thawing method and rapid thawing technique are successful methods where the thawing method applied to Group B damaged spermatozoon viability. The rate of thawing in Group B did not protect the spermatozoa from dissolving or from the harmful effects of cold shock.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%